Subnuclear targeting of the Runx3 tumor suppressor and its epigenetic association with mitotic chromosomes

Sandhya Pande, Syed A. Ali, Christopher Dowdy, Sayyed K. Zaidi, Kosei Ito, Yoshiaki Ito, Martin A. Montecino, Jane B. Lian, Janet L. Stein, Andre J. Van Wijnen, Gary S. Stein

Resultado de la investigación: Review article

30 Citas (Scopus)

Resumen

Runx proteins are tissue-specific transcriptional scaffolds that organize and assemble regulatory complexes at strategic sites of target gene promoters and at intranuclear foci to govern activation or repression. During interphase, fidelity of intranuclear targeting supports the biological activity of Runx1 and Runx2 proteins. Both factors regulate genes involved in cell cycle control and cell growth (e.g., rRNA genes), as well as lineage commitment. Here, we have examined the subcellular regulatory properties of the third Runx member, the tumor suppressor protein Runx3, during interphase and mitosis. Using in situ cellular and biochemical approaches we delineated a subnuclear targeting signal that directs Runx3 to discrete transcriptional foci that are nuclear matrix associated. Chromatin immunoprecipitation results show that Runx3 occupies rRNA promoters during interphase. We also find that Runx3 remains associated with chromosomes during mitosis and localizes with nucleolar organizing regions (NORs), reflecting an interaction with epigenetic potential. Taken together, our study establishes that common mechanisms control the subnuclear distribution and activities of Runx1, Runx2, and Runx3 proteins to support RNA polymerase I and II mediated gene expression during interphase and mitosis.

Idioma originalEnglish
Páginas (desde-hasta)473-479
Número de páginas7
PublicaciónJournal of Cellular Physiology
Volumen218
N.º3
DOI
EstadoPublished - mar 2009

Huella dactilar

Core Binding Factor Alpha 2 Subunit
Core Binding Factor Alpha 1 Subunit
Interphase
Chromosomes
Epigenomics
Tumors
Genes
Mitosis
Association reactions
Core Binding Factor Alpha 3 Subunit
Core Binding Factor alpha Subunits
RNA Polymerase I
Tumor Suppressor Proteins
Neoplasms
RNA Polymerase II
Cell growth
Bioactivity
Nuclear Matrix
Genetic Promoter Regions
Gene expression

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Citar esto

Pande, Sandhya ; Ali, Syed A. ; Dowdy, Christopher ; Zaidi, Sayyed K. ; Ito, Kosei ; Ito, Yoshiaki ; Montecino, Martin A. ; Lian, Jane B. ; Stein, Janet L. ; Van Wijnen, Andre J. ; Stein, Gary S. / Subnuclear targeting of the Runx3 tumor suppressor and its epigenetic association with mitotic chromosomes. En: Journal of Cellular Physiology. 2009 ; Vol. 218, N.º 3. pp. 473-479.
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title = "Subnuclear targeting of the Runx3 tumor suppressor and its epigenetic association with mitotic chromosomes",
abstract = "Runx proteins are tissue-specific transcriptional scaffolds that organize and assemble regulatory complexes at strategic sites of target gene promoters and at intranuclear foci to govern activation or repression. During interphase, fidelity of intranuclear targeting supports the biological activity of Runx1 and Runx2 proteins. Both factors regulate genes involved in cell cycle control and cell growth (e.g., rRNA genes), as well as lineage commitment. Here, we have examined the subcellular regulatory properties of the third Runx member, the tumor suppressor protein Runx3, during interphase and mitosis. Using in situ cellular and biochemical approaches we delineated a subnuclear targeting signal that directs Runx3 to discrete transcriptional foci that are nuclear matrix associated. Chromatin immunoprecipitation results show that Runx3 occupies rRNA promoters during interphase. We also find that Runx3 remains associated with chromosomes during mitosis and localizes with nucleolar organizing regions (NORs), reflecting an interaction with epigenetic potential. Taken together, our study establishes that common mechanisms control the subnuclear distribution and activities of Runx1, Runx2, and Runx3 proteins to support RNA polymerase I and II mediated gene expression during interphase and mitosis.",
author = "Sandhya Pande and Ali, {Syed A.} and Christopher Dowdy and Zaidi, {Sayyed K.} and Kosei Ito and Yoshiaki Ito and Montecino, {Martin A.} and Lian, {Jane B.} and Stein, {Janet L.} and {Van Wijnen}, {Andre J.} and Stein, {Gary S.}",
year = "2009",
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Pande, S, Ali, SA, Dowdy, C, Zaidi, SK, Ito, K, Ito, Y, Montecino, MA, Lian, JB, Stein, JL, Van Wijnen, AJ & Stein, GS 2009, 'Subnuclear targeting of the Runx3 tumor suppressor and its epigenetic association with mitotic chromosomes', Journal of Cellular Physiology, vol. 218, n.º 3, pp. 473-479. https://doi.org/10.1002/jcp.21630

Subnuclear targeting of the Runx3 tumor suppressor and its epigenetic association with mitotic chromosomes. / Pande, Sandhya; Ali, Syed A.; Dowdy, Christopher; Zaidi, Sayyed K.; Ito, Kosei; Ito, Yoshiaki; Montecino, Martin A.; Lian, Jane B.; Stein, Janet L.; Van Wijnen, Andre J.; Stein, Gary S.

En: Journal of Cellular Physiology, Vol. 218, N.º 3, 03.2009, p. 473-479.

Resultado de la investigación: Review article

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T1 - Subnuclear targeting of the Runx3 tumor suppressor and its epigenetic association with mitotic chromosomes

AU - Pande, Sandhya

AU - Ali, Syed A.

AU - Dowdy, Christopher

AU - Zaidi, Sayyed K.

AU - Ito, Kosei

AU - Ito, Yoshiaki

AU - Montecino, Martin A.

AU - Lian, Jane B.

AU - Stein, Janet L.

AU - Van Wijnen, Andre J.

AU - Stein, Gary S.

PY - 2009/3

Y1 - 2009/3

N2 - Runx proteins are tissue-specific transcriptional scaffolds that organize and assemble regulatory complexes at strategic sites of target gene promoters and at intranuclear foci to govern activation or repression. During interphase, fidelity of intranuclear targeting supports the biological activity of Runx1 and Runx2 proteins. Both factors regulate genes involved in cell cycle control and cell growth (e.g., rRNA genes), as well as lineage commitment. Here, we have examined the subcellular regulatory properties of the third Runx member, the tumor suppressor protein Runx3, during interphase and mitosis. Using in situ cellular and biochemical approaches we delineated a subnuclear targeting signal that directs Runx3 to discrete transcriptional foci that are nuclear matrix associated. Chromatin immunoprecipitation results show that Runx3 occupies rRNA promoters during interphase. We also find that Runx3 remains associated with chromosomes during mitosis and localizes with nucleolar organizing regions (NORs), reflecting an interaction with epigenetic potential. Taken together, our study establishes that common mechanisms control the subnuclear distribution and activities of Runx1, Runx2, and Runx3 proteins to support RNA polymerase I and II mediated gene expression during interphase and mitosis.

AB - Runx proteins are tissue-specific transcriptional scaffolds that organize and assemble regulatory complexes at strategic sites of target gene promoters and at intranuclear foci to govern activation or repression. During interphase, fidelity of intranuclear targeting supports the biological activity of Runx1 and Runx2 proteins. Both factors regulate genes involved in cell cycle control and cell growth (e.g., rRNA genes), as well as lineage commitment. Here, we have examined the subcellular regulatory properties of the third Runx member, the tumor suppressor protein Runx3, during interphase and mitosis. Using in situ cellular and biochemical approaches we delineated a subnuclear targeting signal that directs Runx3 to discrete transcriptional foci that are nuclear matrix associated. Chromatin immunoprecipitation results show that Runx3 occupies rRNA promoters during interphase. We also find that Runx3 remains associated with chromosomes during mitosis and localizes with nucleolar organizing regions (NORs), reflecting an interaction with epigenetic potential. Taken together, our study establishes that common mechanisms control the subnuclear distribution and activities of Runx1, Runx2, and Runx3 proteins to support RNA polymerase I and II mediated gene expression during interphase and mitosis.

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