Subnuclear localization and intranuclear trafficking of transcription factors.

Sayyed K. Zaidi, Ricardo F. Medina, Shirwin M. Pockwinse, Rachit Bakshi, Krishna P. Kota, Syed A. Ali, Daniel W. Young, Jeffrey A. Nickerson, Amjad Javed, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein, Gary S. Stein

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

Resumen

Nuclear microenvironments are architecturally organized subnuclear sites where the regulatory machinery for gene expression, replication, and repair resides. This compartmentalization is necessary to attain required stoichiometry for organization and assembly of regulatory complexes for combinatorial control. Combined and methodical application of molecular, cellular, biochemical, and in vivo genetic approaches is required to fully understand complexities of biological control. Here we provide methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.

Idioma originalInglés
Páginas (desde-hasta)77-93
Número de páginas17
PublicaciónMethods in molecular biology (Clifton, N.J.)
Volumen647
EstadoPublicada - 2010

Áreas temáticas de ASJC Scopus

  • Medicina (todo)
  • Genética
  • Biología molecular

Huella

Profundice en los temas de investigación de 'Subnuclear localization and intranuclear trafficking of transcription factors.'. En conjunto forman una huella única.

Citar esto