Sublethal concentrations of waterborne copper induce cellular stress and cell death in zebrafish embryos and larvae

Pedro P. Hernandez, Cristian Undurraga, Viviana E. Gallardo, Natalia Mackenzie, Miguel L. Allende, Ariel E. Reyes

Resultado de la investigación: Article

26 Citas (Scopus)

Resumen

Copper is an essential ion that forms part of the active sites of many proteins. At the same time, an excess of this metal produces free radicals that are toxic for cells and organisms. Fish have been used extensively to study the effects of metals, including copper, present in food or the environment. It has been shown that different metals induce different adaptive responses in adult fish. However, until now, scant information has been available about the responses that are induced by waterborne copper during early life stages of fish. Here, acute toxicity tests and LC50 curves have been generated for zebrafish larvae exposed to dissolved copper sulphate at different concentrations and for different treatment times. We determined that the larvae incorporate and accumulate copper present in the medium in a concentration dependent manner, resulting in changes in gene expression. Using a transgenic fish line that expresses enhanced green fluorescent protein (EGFP) under the hsp70 promoter, we monitored tissue-specific stress responses to waterborne copper by following expression of the reporter. Furthermore, TUNEL assays revealed which tissues are more susceptible to cell death after exposure to copper. Our results establish a framework for the analysis of whole-organism management of excess external copper in developing aquatic animals.

Idioma originalEnglish
Páginas (desde-hasta)7-15
Número de páginas9
PublicaciónBiological Research
Volumen44
N.º1
DOI
EstadoPublished - 8 jun 2011

Huella dactilar

Zebrafish
Cell death
Danio rerio
Larva
cell death
Copper
embryo (animal)
Cell Death
Embryonic Structures
copper
larvae
Fish
Fishes
Metals
metals
fish
Acute Toxicity Tests
Tissue
Copper Sulfate
copper sulfate

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Citar esto

Hernandez, Pedro P. ; Undurraga, Cristian ; Gallardo, Viviana E. ; Mackenzie, Natalia ; Allende, Miguel L. ; Reyes, Ariel E. / Sublethal concentrations of waterborne copper induce cellular stress and cell death in zebrafish embryos and larvae. En: Biological Research. 2011 ; Vol. 44, N.º 1. pp. 7-15.
@article{05997a7162c146febbd07e553851cb8c,
title = "Sublethal concentrations of waterborne copper induce cellular stress and cell death in zebrafish embryos and larvae",
abstract = "Copper is an essential ion that forms part of the active sites of many proteins. At the same time, an excess of this metal produces free radicals that are toxic for cells and organisms. Fish have been used extensively to study the effects of metals, including copper, present in food or the environment. It has been shown that different metals induce different adaptive responses in adult fish. However, until now, scant information has been available about the responses that are induced by waterborne copper during early life stages of fish. Here, acute toxicity tests and LC50 curves have been generated for zebrafish larvae exposed to dissolved copper sulphate at different concentrations and for different treatment times. We determined that the larvae incorporate and accumulate copper present in the medium in a concentration dependent manner, resulting in changes in gene expression. Using a transgenic fish line that expresses enhanced green fluorescent protein (EGFP) under the hsp70 promoter, we monitored tissue-specific stress responses to waterborne copper by following expression of the reporter. Furthermore, TUNEL assays revealed which tissues are more susceptible to cell death after exposure to copper. Our results establish a framework for the analysis of whole-organism management of excess external copper in developing aquatic animals.",
keywords = "Acute exposure, Apoptosis, Copper, Early life stage, Heat shock, Protein-70, Zebrafish",
author = "Hernandez, {Pedro P.} and Cristian Undurraga and Gallardo, {Viviana E.} and Natalia Mackenzie and Allende, {Miguel L.} and Reyes, {Ariel E.}",
year = "2011",
month = "6",
day = "8",
doi = "10.4067/S0716-97602011000100002",
language = "English",
volume = "44",
pages = "7--15",
journal = "Biological Research",
issn = "0716-9760",
publisher = "Society of Biology of Chile",
number = "1",

}

Sublethal concentrations of waterborne copper induce cellular stress and cell death in zebrafish embryos and larvae. / Hernandez, Pedro P.; Undurraga, Cristian; Gallardo, Viviana E.; Mackenzie, Natalia; Allende, Miguel L.; Reyes, Ariel E.

En: Biological Research, Vol. 44, N.º 1, 08.06.2011, p. 7-15.

Resultado de la investigación: Article

TY - JOUR

T1 - Sublethal concentrations of waterborne copper induce cellular stress and cell death in zebrafish embryos and larvae

AU - Hernandez, Pedro P.

AU - Undurraga, Cristian

AU - Gallardo, Viviana E.

AU - Mackenzie, Natalia

AU - Allende, Miguel L.

AU - Reyes, Ariel E.

PY - 2011/6/8

Y1 - 2011/6/8

N2 - Copper is an essential ion that forms part of the active sites of many proteins. At the same time, an excess of this metal produces free radicals that are toxic for cells and organisms. Fish have been used extensively to study the effects of metals, including copper, present in food or the environment. It has been shown that different metals induce different adaptive responses in adult fish. However, until now, scant information has been available about the responses that are induced by waterborne copper during early life stages of fish. Here, acute toxicity tests and LC50 curves have been generated for zebrafish larvae exposed to dissolved copper sulphate at different concentrations and for different treatment times. We determined that the larvae incorporate and accumulate copper present in the medium in a concentration dependent manner, resulting in changes in gene expression. Using a transgenic fish line that expresses enhanced green fluorescent protein (EGFP) under the hsp70 promoter, we monitored tissue-specific stress responses to waterborne copper by following expression of the reporter. Furthermore, TUNEL assays revealed which tissues are more susceptible to cell death after exposure to copper. Our results establish a framework for the analysis of whole-organism management of excess external copper in developing aquatic animals.

AB - Copper is an essential ion that forms part of the active sites of many proteins. At the same time, an excess of this metal produces free radicals that are toxic for cells and organisms. Fish have been used extensively to study the effects of metals, including copper, present in food or the environment. It has been shown that different metals induce different adaptive responses in adult fish. However, until now, scant information has been available about the responses that are induced by waterborne copper during early life stages of fish. Here, acute toxicity tests and LC50 curves have been generated for zebrafish larvae exposed to dissolved copper sulphate at different concentrations and for different treatment times. We determined that the larvae incorporate and accumulate copper present in the medium in a concentration dependent manner, resulting in changes in gene expression. Using a transgenic fish line that expresses enhanced green fluorescent protein (EGFP) under the hsp70 promoter, we monitored tissue-specific stress responses to waterborne copper by following expression of the reporter. Furthermore, TUNEL assays revealed which tissues are more susceptible to cell death after exposure to copper. Our results establish a framework for the analysis of whole-organism management of excess external copper in developing aquatic animals.

KW - Acute exposure

KW - Apoptosis

KW - Copper

KW - Early life stage

KW - Heat shock

KW - Protein-70

KW - Zebrafish

UR - http://www.scopus.com/inward/record.url?scp=79957945175&partnerID=8YFLogxK

U2 - 10.4067/S0716-97602011000100002

DO - 10.4067/S0716-97602011000100002

M3 - Article

C2 - 21720676

AN - SCOPUS:79957945175

VL - 44

SP - 7

EP - 15

JO - Biological Research

JF - Biological Research

SN - 0716-9760

IS - 1

ER -