TY - JOUR
T1 - Study of the interaction of folic acid-modified gold nanorods and fibrinogen through microfluidics
T2 - Implications for protein adsorption, incorporation and viability of cancer cells
AU - Orellana, Nacaroha
AU - Palma, Sujey
AU - Torres, Estefania
AU - Cordero, María Luisa
AU - Vio, Valentina
AU - Ruso, Juan M.
AU - Juárez, Josué
AU - Topete, Antonio
AU - Araya, Eyleen
AU - Vasquez-Contreras, Rodrigo
AU - Kogan, Marcelo J.
AU - Hassan, Natalia
N1 - Publisher Copyright:
© The Royal Society of Chemistry.
PY - 2021/11/14
Y1 - 2021/11/14
N2 - Gold nanoparticles (GNPs) are an attractive nanomaterial for potential applications in therapy and diagnostics due to their capability to direct toward specific sites in the organism. However, when exposed to plasma, GNPs can interact with different biomolecules that form a dynamic nano-bio interface called a "protein corona"(PC). Remarkably, the PC could affect multiple biological processes, such as cell targeting and uptake, cytotoxicity, and nanoparticle (NP) clearance. The interaction of nanomaterials with plasmatic proteins has been widely studied under bulk conditions, however, under dynamic conditions, it has just recently been explored. Thus, to mimic a dynamic natural environment found in arteries and veins, microfluidic devices were used. In this work, gold nanorods (GNRs) were synthesized and conjugated with polyethylene glycol (PEG) to reduce their interaction with plasma proteins and increase their biocompatibility. Then, GNRs were functionalized with folic acid, a targeting ligand typically used to recognize tumor cells. The resulting nanosystem was exposed to fibrinogen (FB) to study the development and biological impact of PC formation through two strategies: bulk and laminar flow conditions. The obtained nanosystems were characterized by absorption spectrophotometry, DLS, laser Doppler microelectrophoresis, neutron activation analysis, circular dichroism spectroscopy and TEM. Finally, cell viability and cellular uptake assays were performed to study the influence of the PC on the cell viability and delivery of nanosystems. This journal is
AB - Gold nanoparticles (GNPs) are an attractive nanomaterial for potential applications in therapy and diagnostics due to their capability to direct toward specific sites in the organism. However, when exposed to plasma, GNPs can interact with different biomolecules that form a dynamic nano-bio interface called a "protein corona"(PC). Remarkably, the PC could affect multiple biological processes, such as cell targeting and uptake, cytotoxicity, and nanoparticle (NP) clearance. The interaction of nanomaterials with plasmatic proteins has been widely studied under bulk conditions, however, under dynamic conditions, it has just recently been explored. Thus, to mimic a dynamic natural environment found in arteries and veins, microfluidic devices were used. In this work, gold nanorods (GNRs) were synthesized and conjugated with polyethylene glycol (PEG) to reduce their interaction with plasma proteins and increase their biocompatibility. Then, GNRs were functionalized with folic acid, a targeting ligand typically used to recognize tumor cells. The resulting nanosystem was exposed to fibrinogen (FB) to study the development and biological impact of PC formation through two strategies: bulk and laminar flow conditions. The obtained nanosystems were characterized by absorption spectrophotometry, DLS, laser Doppler microelectrophoresis, neutron activation analysis, circular dichroism spectroscopy and TEM. Finally, cell viability and cellular uptake assays were performed to study the influence of the PC on the cell viability and delivery of nanosystems. This journal is
UR - http://www.scopus.com/inward/record.url?scp=85119340658&partnerID=8YFLogxK
U2 - 10.1039/d1nr03179a
DO - 10.1039/d1nr03179a
M3 - Article
AN - SCOPUS:85119340658
SN - 2040-3364
VL - 13
SP - 17807
EP - 17821
JO - Nanoscale
JF - Nanoscale
IS - 42
ER -