Studies on fish liver protein synthesis-I. Isolation and characterizatin of shark liver transfer ribonucleic acid

Manuel Krauskopf, Alejandro Araya, Simon Litvak

Resultado de la investigación: Article

7 Citas (Scopus)

Resumen

1. 1. The isolation of tRNA from the liver of the shark Mustelus mento is described. The prcedure involves phenol extraction, chromatography on DEAE-cellulose and isopropanol fractionation. The obtained fractions were analyzed by plyacrylamide gel electrophoresis. 2. 2. The tRNA was shown to have an intact 3' end and to accept the four amino acids tested with homologous aminoacyl-tRNA synthetases. 3. 3. Analytical centrifuge analysis gave a sedimentation coefficient of S20,w=4·01. 4. 4. Thermal denaturation profiles were compared to other tRNAs.

Idioma originalEnglish
PublicaciónComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volumen48
N.º4
DOI
EstadoPublished - 15 ago 1974

Huella dactilar

Fish Proteins
Sharks
Transfer RNA
Liver
Amino Acyl-tRNA Synthetases
DEAE-Cellulose Chromatography
2-Propanol
Phenol
Electrophoresis
Hot Temperature
Gels
Amino Acids

ASJC Scopus subject areas

  • Medicine(all)

Citar esto

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Studies on fish liver protein synthesis-I. Isolation and characterizatin of shark liver transfer ribonucleic acid. / Krauskopf, Manuel; Araya, Alejandro; Litvak, Simon.

En: Comparative Biochemistry and Physiology -- Part B: Biochemistry and, Vol. 48, N.º 4, 15.08.1974.

Resultado de la investigación: Article

TY - JOUR

T1 - Studies on fish liver protein synthesis-I. Isolation and characterizatin of shark liver transfer ribonucleic acid

AU - Krauskopf, Manuel

AU - Araya, Alejandro

AU - Litvak, Simon

PY - 1974/8/15

Y1 - 1974/8/15

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AB - 1. 1. The isolation of tRNA from the liver of the shark Mustelus mento is described. The prcedure involves phenol extraction, chromatography on DEAE-cellulose and isopropanol fractionation. The obtained fractions were analyzed by plyacrylamide gel electrophoresis. 2. 2. The tRNA was shown to have an intact 3' end and to accept the four amino acids tested with homologous aminoacyl-tRNA synthetases. 3. 3. Analytical centrifuge analysis gave a sedimentation coefficient of S20,w=4·01. 4. 4. Thermal denaturation profiles were compared to other tRNAs.

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KW - poikilotherm

KW - protein synthesis

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KW - tRNA

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