Studies on fish liver protein synthesis-I. Isolation and characterizatin of shark liver transfer ribonucleic acid

Manuel Krauskopf, Alejandro Araya, Simon Litvak

Resultado de la investigación: Contribución a una revistaArtículo

7 Citas (Scopus)

Resumen

1. 1. The isolation of tRNA from the liver of the shark Mustelus mento is described. The prcedure involves phenol extraction, chromatography on DEAE-cellulose and isopropanol fractionation. The obtained fractions were analyzed by plyacrylamide gel electrophoresis. 2. 2. The tRNA was shown to have an intact 3' end and to accept the four amino acids tested with homologous aminoacyl-tRNA synthetases. 3. 3. Analytical centrifuge analysis gave a sedimentation coefficient of S20,w=4·01. 4. 4. Thermal denaturation profiles were compared to other tRNAs.

Idioma originalInglés
PublicaciónComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volumen48
N.º4
DOI
EstadoPublicada - 15 ago 1974

Áreas temáticas de ASJC Scopus

  • Medicina (todo)

Huella Profundice en los temas de investigación de 'Studies on fish liver protein synthesis-I. Isolation and characterizatin of shark liver transfer ribonucleic acid'. En conjunto forman una huella única.

  • Citar esto