Structure and processing of yeast precursor tRNAs containing intervening sequences

P. Z. O'Farrell, B. Cordell, P. Valenzuela, W. J. Rutter, H. M. Goodman

Resultado de la investigación: Article

71 Citas (Scopus)

Resumen

We have isolated a precursor of yeast tRNATyr and shown that it contains an intervening sequence identical to that found in the gene for tRNATyr. The conformation of pre-tRNATyr is similar to that of mature tRNATyr except for the anticodon loop. The loop is sensitive to endonucleolytic cleavage by S1 nuclease near to the ends of the intervening sequence. This pre-tRNA is functionally inactive as it cannot be aminoacylated and the anticodon is not accessible for hydrogen bonding. A crude nuclear extract from yeast contains an excision-ligase activity which will process pre-tRNATyr into mature tRNATyr.

Idioma originalEnglish
Páginas (desde-hasta)438-445
Número de páginas8
PublicaciónNature
Volumen274
N.º5670
DOI
EstadoPublished - 1 dic 1978

Huella dactilar

RNA, Transfer, Tyr
RNA Precursors
Introns
Yeasts
Anticodon
Hydrogen Bonding
Ligases
Complex Mixtures

ASJC Scopus subject areas

  • General

Citar esto

O'Farrell, P. Z., Cordell, B., Valenzuela, P., Rutter, W. J., & Goodman, H. M. (1978). Structure and processing of yeast precursor tRNAs containing intervening sequences. Nature, 274(5670), 438-445. https://doi.org/10.1038/274438a0
O'Farrell, P. Z. ; Cordell, B. ; Valenzuela, P. ; Rutter, W. J. ; Goodman, H. M. / Structure and processing of yeast precursor tRNAs containing intervening sequences. En: Nature. 1978 ; Vol. 274, N.º 5670. pp. 438-445.
@article{a540cc8aaad947108bed28dca854726f,
title = "Structure and processing of yeast precursor tRNAs containing intervening sequences",
abstract = "We have isolated a precursor of yeast tRNATyr and shown that it contains an intervening sequence identical to that found in the gene for tRNATyr. The conformation of pre-tRNATyr is similar to that of mature tRNATyr except for the anticodon loop. The loop is sensitive to endonucleolytic cleavage by S1 nuclease near to the ends of the intervening sequence. This pre-tRNA is functionally inactive as it cannot be aminoacylated and the anticodon is not accessible for hydrogen bonding. A crude nuclear extract from yeast contains an excision-ligase activity which will process pre-tRNATyr into mature tRNATyr.",
author = "O'Farrell, {P. Z.} and B. Cordell and P. Valenzuela and Rutter, {W. J.} and Goodman, {H. M.}",
year = "1978",
month = "12",
day = "1",
doi = "10.1038/274438a0",
language = "English",
volume = "274",
pages = "438--445",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "5670",

}

O'Farrell, PZ, Cordell, B, Valenzuela, P, Rutter, WJ & Goodman, HM 1978, 'Structure and processing of yeast precursor tRNAs containing intervening sequences', Nature, vol. 274, n.º 5670, pp. 438-445. https://doi.org/10.1038/274438a0

Structure and processing of yeast precursor tRNAs containing intervening sequences. / O'Farrell, P. Z.; Cordell, B.; Valenzuela, P.; Rutter, W. J.; Goodman, H. M.

En: Nature, Vol. 274, N.º 5670, 01.12.1978, p. 438-445.

Resultado de la investigación: Article

TY - JOUR

T1 - Structure and processing of yeast precursor tRNAs containing intervening sequences

AU - O'Farrell, P. Z.

AU - Cordell, B.

AU - Valenzuela, P.

AU - Rutter, W. J.

AU - Goodman, H. M.

PY - 1978/12/1

Y1 - 1978/12/1

N2 - We have isolated a precursor of yeast tRNATyr and shown that it contains an intervening sequence identical to that found in the gene for tRNATyr. The conformation of pre-tRNATyr is similar to that of mature tRNATyr except for the anticodon loop. The loop is sensitive to endonucleolytic cleavage by S1 nuclease near to the ends of the intervening sequence. This pre-tRNA is functionally inactive as it cannot be aminoacylated and the anticodon is not accessible for hydrogen bonding. A crude nuclear extract from yeast contains an excision-ligase activity which will process pre-tRNATyr into mature tRNATyr.

AB - We have isolated a precursor of yeast tRNATyr and shown that it contains an intervening sequence identical to that found in the gene for tRNATyr. The conformation of pre-tRNATyr is similar to that of mature tRNATyr except for the anticodon loop. The loop is sensitive to endonucleolytic cleavage by S1 nuclease near to the ends of the intervening sequence. This pre-tRNA is functionally inactive as it cannot be aminoacylated and the anticodon is not accessible for hydrogen bonding. A crude nuclear extract from yeast contains an excision-ligase activity which will process pre-tRNATyr into mature tRNATyr.

UR - http://www.scopus.com/inward/record.url?scp=0017852413&partnerID=8YFLogxK

U2 - 10.1038/274438a0

DO - 10.1038/274438a0

M3 - Article

C2 - 353564

AN - SCOPUS:0017852413

VL - 274

SP - 438

EP - 445

JO - Nature

JF - Nature

SN - 0028-0836

IS - 5670

ER -

O'Farrell PZ, Cordell B, Valenzuela P, Rutter WJ, Goodman HM. Structure and processing of yeast precursor tRNAs containing intervening sequences. Nature. 1978 dic 1;274(5670):438-445. https://doi.org/10.1038/274438a0