Spontaneous excision of the Salmonella enterica serovar enteritidis-specific defective prophage-like element φSE14

Carlos A. Santiviago, Carlos J. Blondel, Carolina P. Quezada, Cecilia A. Silva, Pia M. Tobar, Steffen Porwollik, Michael McClelland, Helene L. Andrews-Polymenis, Cecilia S. Toro, Mercedes Zaldívar, Inés Contreras

Resultado de la investigación: Article

19 Citas (Scopus)

Resumen

Salmonella enterica serovar Enteritidis has emerged as a major health problem worldwide in the last few decades. DNA loci unique to S. Enteritidis can provide markers for detection of this pathogen and may reveal pathogenic mechanisms restricted to this serovar. An in silico comparison of 16 Salmonella genomic sequences revealed the presence of an ∼12.5-kb genomic island (GEI) specific to the sequenced S. Enteritidis strain NCTC13349. The GEI is inserted at the 5′ end of gene ydaO (SEN1377), is flanked by 308-bp imperfect direct repeats (attL and attR), and includes 21 open reading frames (SEN1378 to SEN1398), encoding primarily phage-related proteins. Accordingly, this GEI has been annotated as the defective prophage SE14 in the genome of strain NCTC13349. The genetic structure and location of φSE14 are conserved in 99 of 103 wild-type strains of S. Enteritidis studied here, including reference strains NCTC13349 and LK5. Notably, an extra-chromosomal circular form of φSE14 was detected in every strain carrying this island. The presence of attP sites in the circular forms detected in NCTC13349 and LK5 was confirmed. In addition, we observed spontaneous loss of a tetRA-tagged version of φSE14, leaving an empty attB site in the genome of strain NCTC13349. Collectively, these results demonstrate that φSE14 is an unstable genetic element that undergoes spontaneous excision under standard growth conditions. An internal fragment of φSE14 designated Sdf I has been used as a serovar-specific genetic marker in PCR-based detection systems and as a tool to determine S. Enteritidis levels in experimental infections. The instability of this region may require a reassessment of its suitability for such applications.

Idioma originalEnglish
Páginas (desde-hasta)2246-2254
Número de páginas9
PublicaciónJournal of Bacteriology
Volumen192
N.º8
DOI
EstadoPublished - abr 2010

Huella dactilar

Prophages
Genomic Islands
Salmonella enteritidis
Genome
Genetic Structures
Nucleic Acid Repetitive Sequences
Genetic Markers
Islands
Salmonella
Computer Simulation
Bacteriophages
Open Reading Frames
Polymerase Chain Reaction
DNA
Growth
Infection
Genes
Proteins
Serogroup

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Citar esto

Santiviago, C. A., Blondel, C. J., Quezada, C. P., Silva, C. A., Tobar, P. M., Porwollik, S., ... Contreras, I. (2010). Spontaneous excision of the Salmonella enterica serovar enteritidis-specific defective prophage-like element φSE14. Journal of Bacteriology, 192(8), 2246-2254. https://doi.org/10.1128/JB.00270-09
Santiviago, Carlos A. ; Blondel, Carlos J. ; Quezada, Carolina P. ; Silva, Cecilia A. ; Tobar, Pia M. ; Porwollik, Steffen ; McClelland, Michael ; Andrews-Polymenis, Helene L. ; Toro, Cecilia S. ; Zaldívar, Mercedes ; Contreras, Inés. / Spontaneous excision of the Salmonella enterica serovar enteritidis-specific defective prophage-like element φSE14. En: Journal of Bacteriology. 2010 ; Vol. 192, N.º 8. pp. 2246-2254.
@article{582571cda2614b699b802fd3cafcde49,
title = "Spontaneous excision of the Salmonella enterica serovar enteritidis-specific defective prophage-like element φSE14",
abstract = "Salmonella enterica serovar Enteritidis has emerged as a major health problem worldwide in the last few decades. DNA loci unique to S. Enteritidis can provide markers for detection of this pathogen and may reveal pathogenic mechanisms restricted to this serovar. An in silico comparison of 16 Salmonella genomic sequences revealed the presence of an ∼12.5-kb genomic island (GEI) specific to the sequenced S. Enteritidis strain NCTC13349. The GEI is inserted at the 5′ end of gene ydaO (SEN1377), is flanked by 308-bp imperfect direct repeats (attL and attR), and includes 21 open reading frames (SEN1378 to SEN1398), encoding primarily phage-related proteins. Accordingly, this GEI has been annotated as the defective prophage SE14 in the genome of strain NCTC13349. The genetic structure and location of φSE14 are conserved in 99 of 103 wild-type strains of S. Enteritidis studied here, including reference strains NCTC13349 and LK5. Notably, an extra-chromosomal circular form of φSE14 was detected in every strain carrying this island. The presence of attP sites in the circular forms detected in NCTC13349 and LK5 was confirmed. In addition, we observed spontaneous loss of a tetRA-tagged version of φSE14, leaving an empty attB site in the genome of strain NCTC13349. Collectively, these results demonstrate that φSE14 is an unstable genetic element that undergoes spontaneous excision under standard growth conditions. An internal fragment of φSE14 designated Sdf I has been used as a serovar-specific genetic marker in PCR-based detection systems and as a tool to determine S. Enteritidis levels in experimental infections. The instability of this region may require a reassessment of its suitability for such applications.",
author = "Santiviago, {Carlos A.} and Blondel, {Carlos J.} and Quezada, {Carolina P.} and Silva, {Cecilia A.} and Tobar, {Pia M.} and Steffen Porwollik and Michael McClelland and Andrews-Polymenis, {Helene L.} and Toro, {Cecilia S.} and Mercedes Zald{\'i}var and In{\'e}s Contreras",
year = "2010",
month = "4",
doi = "10.1128/JB.00270-09",
language = "English",
volume = "192",
pages = "2246--2254",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "8",

}

Santiviago, CA, Blondel, CJ, Quezada, CP, Silva, CA, Tobar, PM, Porwollik, S, McClelland, M, Andrews-Polymenis, HL, Toro, CS, Zaldívar, M & Contreras, I 2010, 'Spontaneous excision of the Salmonella enterica serovar enteritidis-specific defective prophage-like element φSE14', Journal of Bacteriology, vol. 192, n.º 8, pp. 2246-2254. https://doi.org/10.1128/JB.00270-09

Spontaneous excision of the Salmonella enterica serovar enteritidis-specific defective prophage-like element φSE14. / Santiviago, Carlos A.; Blondel, Carlos J.; Quezada, Carolina P.; Silva, Cecilia A.; Tobar, Pia M.; Porwollik, Steffen; McClelland, Michael; Andrews-Polymenis, Helene L.; Toro, Cecilia S.; Zaldívar, Mercedes; Contreras, Inés.

En: Journal of Bacteriology, Vol. 192, N.º 8, 04.2010, p. 2246-2254.

Resultado de la investigación: Article

TY - JOUR

T1 - Spontaneous excision of the Salmonella enterica serovar enteritidis-specific defective prophage-like element φSE14

AU - Santiviago, Carlos A.

AU - Blondel, Carlos J.

AU - Quezada, Carolina P.

AU - Silva, Cecilia A.

AU - Tobar, Pia M.

AU - Porwollik, Steffen

AU - McClelland, Michael

AU - Andrews-Polymenis, Helene L.

AU - Toro, Cecilia S.

AU - Zaldívar, Mercedes

AU - Contreras, Inés

PY - 2010/4

Y1 - 2010/4

N2 - Salmonella enterica serovar Enteritidis has emerged as a major health problem worldwide in the last few decades. DNA loci unique to S. Enteritidis can provide markers for detection of this pathogen and may reveal pathogenic mechanisms restricted to this serovar. An in silico comparison of 16 Salmonella genomic sequences revealed the presence of an ∼12.5-kb genomic island (GEI) specific to the sequenced S. Enteritidis strain NCTC13349. The GEI is inserted at the 5′ end of gene ydaO (SEN1377), is flanked by 308-bp imperfect direct repeats (attL and attR), and includes 21 open reading frames (SEN1378 to SEN1398), encoding primarily phage-related proteins. Accordingly, this GEI has been annotated as the defective prophage SE14 in the genome of strain NCTC13349. The genetic structure and location of φSE14 are conserved in 99 of 103 wild-type strains of S. Enteritidis studied here, including reference strains NCTC13349 and LK5. Notably, an extra-chromosomal circular form of φSE14 was detected in every strain carrying this island. The presence of attP sites in the circular forms detected in NCTC13349 and LK5 was confirmed. In addition, we observed spontaneous loss of a tetRA-tagged version of φSE14, leaving an empty attB site in the genome of strain NCTC13349. Collectively, these results demonstrate that φSE14 is an unstable genetic element that undergoes spontaneous excision under standard growth conditions. An internal fragment of φSE14 designated Sdf I has been used as a serovar-specific genetic marker in PCR-based detection systems and as a tool to determine S. Enteritidis levels in experimental infections. The instability of this region may require a reassessment of its suitability for such applications.

AB - Salmonella enterica serovar Enteritidis has emerged as a major health problem worldwide in the last few decades. DNA loci unique to S. Enteritidis can provide markers for detection of this pathogen and may reveal pathogenic mechanisms restricted to this serovar. An in silico comparison of 16 Salmonella genomic sequences revealed the presence of an ∼12.5-kb genomic island (GEI) specific to the sequenced S. Enteritidis strain NCTC13349. The GEI is inserted at the 5′ end of gene ydaO (SEN1377), is flanked by 308-bp imperfect direct repeats (attL and attR), and includes 21 open reading frames (SEN1378 to SEN1398), encoding primarily phage-related proteins. Accordingly, this GEI has been annotated as the defective prophage SE14 in the genome of strain NCTC13349. The genetic structure and location of φSE14 are conserved in 99 of 103 wild-type strains of S. Enteritidis studied here, including reference strains NCTC13349 and LK5. Notably, an extra-chromosomal circular form of φSE14 was detected in every strain carrying this island. The presence of attP sites in the circular forms detected in NCTC13349 and LK5 was confirmed. In addition, we observed spontaneous loss of a tetRA-tagged version of φSE14, leaving an empty attB site in the genome of strain NCTC13349. Collectively, these results demonstrate that φSE14 is an unstable genetic element that undergoes spontaneous excision under standard growth conditions. An internal fragment of φSE14 designated Sdf I has been used as a serovar-specific genetic marker in PCR-based detection systems and as a tool to determine S. Enteritidis levels in experimental infections. The instability of this region may require a reassessment of its suitability for such applications.

UR - http://www.scopus.com/inward/record.url?scp=77950644514&partnerID=8YFLogxK

U2 - 10.1128/JB.00270-09

DO - 10.1128/JB.00270-09

M3 - Article

C2 - 20172996

AN - SCOPUS:77950644514

VL - 192

SP - 2246

EP - 2254

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 8

ER -