TY - JOUR
T1 - Sperm polymorphism and genetic divergence in the mussel Perumytilus purpuratus
AU - Briones, Carolina
AU - Guiñez, Ricardo
AU - Garrido, Orlando
AU - Oyarzún, Pablo A.
AU - Toro, Jorge E.
AU - Pérez, Montse
N1 - Funding Information:
Acknowledgments Financial support was provided by the Fondo Nacional de Ciencia y Tecnología FONDECYT (Project 1101007 to RG), Comisión Nacional de Ciencia y Tecnología CONICYT (Beca de apoyo para la realización de tesis doctoral 2008 awarded to CB), and the Programa de Mejoramiento de la Calidad y la Equidad de la Educación Superior MECESUP de la República de Chile (Project ANT0711, Universidad de Antofagasta). Special thanks go to Cristian Briones for schematic drawing.
PY - 2012/8/1
Y1 - 2012/8/1
N2 - For the first time in a marine bivalve species, geographic intraspecific variation in sperm morphology in the Chilean mussel, Perumytilus purpuratus, is reported. Samples were obtained in December 2010 from the rocky intertidal at three locations: Antofagasta (23°44′01.08″S, 70°26′26.11″W), Valparaíso (32°57′23.07″S, 71°33′01.86″W), and Valdivia (39°50′44.26″S, 73°25′51.99″W). Scanning electron microscopy was used to describe sperm traits, and genetic data were obtained by sequencing a 16S mtDNA 427-bp fragment. The results showed geographic intraspecific variation with two statistically significant patterns of genetically differentiated sperm morphologies. One genetic clade was found for Antofagasta and Valparaíso, displaying a unique sperm morphotype with a short acrosome accounting for 26 % of the head length, and the other from Valdivia, with a larger acrosome occupying 47 % of the head length. These results will lead to new research to distinguish among alternative hypotheses: the presence of cryptic species, incipient speciation, or a polymorphism in sperm traits of P. purpuratus.
AB - For the first time in a marine bivalve species, geographic intraspecific variation in sperm morphology in the Chilean mussel, Perumytilus purpuratus, is reported. Samples were obtained in December 2010 from the rocky intertidal at three locations: Antofagasta (23°44′01.08″S, 70°26′26.11″W), Valparaíso (32°57′23.07″S, 71°33′01.86″W), and Valdivia (39°50′44.26″S, 73°25′51.99″W). Scanning electron microscopy was used to describe sperm traits, and genetic data were obtained by sequencing a 16S mtDNA 427-bp fragment. The results showed geographic intraspecific variation with two statistically significant patterns of genetically differentiated sperm morphologies. One genetic clade was found for Antofagasta and Valparaíso, displaying a unique sperm morphotype with a short acrosome accounting for 26 % of the head length, and the other from Valdivia, with a larger acrosome occupying 47 % of the head length. These results will lead to new research to distinguish among alternative hypotheses: the presence of cryptic species, incipient speciation, or a polymorphism in sperm traits of P. purpuratus.
UR - http://www.scopus.com/inward/record.url?scp=84864378175&partnerID=8YFLogxK
U2 - 10.1007/s00227-012-1952-3
DO - 10.1007/s00227-012-1952-3
M3 - Article
AN - SCOPUS:84864378175
SN - 0025-3162
VL - 159
SP - 1865
EP - 1870
JO - Marine Biology
JF - Marine Biology
IS - 8
ER -
