TY - JOUR
T1 - Specific DNA probes for the identification of the fish pathogen, Renibacterium salmoninarum
AU - León, G.
AU - Martinez, M. A.
AU - Etchegaray, J. P.
AU - Vera, M. I.
AU - Figueroa, J.
AU - Krauskopf, M.
PY - 1994/3/1
Y1 - 1994/3/1
N2 - To obtain specific DNA probes for the identification of the fish pathogen, Renibacterium salmoninarum, a discriminatory recombinant DNA library was constructed using selective fragments of the bacterial genome. Three renibacterial clones, pMAM29, pMAM46 and pMAM77, containing 149, 73, and 154 bp respectively, were isolated and characterized. The specificity of the probes was confirmed by dot-blot and Southern hybridization analyses. Bacterial hybridization experiments revealed that pMAM29 discriminates the R. salmoninarum genome from that of other fish pathogens such as Aeromonas salmonicida, Yersinia ruckeri, Flexibacter columnaris, Lactobacillus piscicola, Vibrio ordalii, Vibrio anguillarum and Aeromonas hydrophila. Thus, this probe may provide a new means to diagnose bacterial kidney disease in asymptomatic fish and ova.
AB - To obtain specific DNA probes for the identification of the fish pathogen, Renibacterium salmoninarum, a discriminatory recombinant DNA library was constructed using selective fragments of the bacterial genome. Three renibacterial clones, pMAM29, pMAM46 and pMAM77, containing 149, 73, and 154 bp respectively, were isolated and characterized. The specificity of the probes was confirmed by dot-blot and Southern hybridization analyses. Bacterial hybridization experiments revealed that pMAM29 discriminates the R. salmoninarum genome from that of other fish pathogens such as Aeromonas salmonicida, Yersinia ruckeri, Flexibacter columnaris, Lactobacillus piscicola, Vibrio ordalii, Vibrio anguillarum and Aeromonas hydrophila. Thus, this probe may provide a new means to diagnose bacterial kidney disease in asymptomatic fish and ova.
KW - Bacterial kidney disease
KW - fish pathogens
KW - probes
KW - Renibacterium salmoninarum
KW - salmon
UR - http://www.scopus.com/inward/record.url?scp=0028038884&partnerID=8YFLogxK
U2 - 10.1007/BF00360876
DO - 10.1007/BF00360876
M3 - Article
AN - SCOPUS:0028038884
SN - 0959-3993
VL - 10
SP - 149
EP - 153
JO - World Journal of Microbiology and Biotechnology
JF - World Journal of Microbiology and Biotechnology
IS - 2
ER -