Specific DNA probes for the identification of the fish pathogen, Renibacterium salmoninarum

G. León, M. A. Martinez, J. P. Etchegaray, M. I. Vera, J. Figueroa, M. Krauskopf

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

13 Citas (Scopus)

Resumen

To obtain specific DNA probes for the identification of the fish pathogen, Renibacterium salmoninarum, a discriminatory recombinant DNA library was constructed using selective fragments of the bacterial genome. Three renibacterial clones, pMAM29, pMAM46 and pMAM77, containing 149, 73, and 154 bp respectively, were isolated and characterized. The specificity of the probes was confirmed by dot-blot and Southern hybridization analyses. Bacterial hybridization experiments revealed that pMAM29 discriminates the R. salmoninarum genome from that of other fish pathogens such as Aeromonas salmonicida, Yersinia ruckeri, Flexibacter columnaris, Lactobacillus piscicola, Vibrio ordalii, Vibrio anguillarum and Aeromonas hydrophila. Thus, this probe may provide a new means to diagnose bacterial kidney disease in asymptomatic fish and ova.

Idioma originalInglés
Páginas (desde-hasta)149-153
Número de páginas5
PublicaciónWorld Journal of Microbiology & Biotechnology
Volumen10
N.º2
DOI
EstadoPublicada - 1 mar 1994

Áreas temáticas de ASJC Scopus

  • Biotecnología
  • Fisiología
  • Microbiología y biotecnología aplicadas

Huella

Profundice en los temas de investigación de 'Specific DNA probes for the identification of the fish pathogen, Renibacterium salmoninarum'. En conjunto forman una huella única.

Citar esto