Soybean Meal Induces Intestinal Inflammation in Zebrafish Larvae

Manuel I. Hedrera, Jorge A. Galdames, Maria F. Jimenez-Reyes, Ariel E. Reyes, Ruben Avendaño-Herrera, Jaime Romero, Carmen G. Feijóo

Resultado de la investigación: Article

69 Citas (Scopus)

Resumen

The necessary replacement of fish meal with other protein source in diets of commercially important fish has prompted the study of the effect of the inclusion of different vegetable proteins sources on growth performance and on the gastro-intestinal tract. Currently, soybean meal is the primary protein source as a fish meal replacement because of its low price and high availability. Likewise, it is been documented that the ingestion of soybean meal by several fish species, such as salmonids and carp, triggers a type of intestinal inflammation called enteritis. In this paper, we analyzed the effects of the ingestion of soybean meal and two of its components, soy protein and soy saponin, on zebrafish to establish the basis for using zebrafish larvae as a model for fish nutrition. We took advantage of the existence of different transgenic lines, which allowed us to perform in vivo analysis. Our results indicated that larvae that were feed with soybean meal developed a clear intestinal inflammation as early as two day after beginning the diet. Moreover, we determined that is not the soy protein present in the diet but the soy saponin that is primarily responsible for triggering the immune response. These findings support the use of zebrafish screening assays to identify novel ingredients that would to improved current fish diets or would formulate new ones.

Idioma originalEnglish
Número de artículoe69983
PublicaciónPLoS ONE
Volumen8
N.º7
DOI
EstadoPublished - 23 jul 2013

Huella dactilar

Zebrafish
Danio rerio
Soybeans
Fish
Larva
soybean meal
Meals
Nutrition
Fishes
inflammation
protein sources
Inflammation
larvae
soy protein
fish meal
saponins
diet
Diet
Soybean Proteins
fish

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Citar esto

Hedrera, Manuel I. ; Galdames, Jorge A. ; Jimenez-Reyes, Maria F. ; Reyes, Ariel E. ; Avendaño-Herrera, Ruben ; Romero, Jaime ; Feijóo, Carmen G. / Soybean Meal Induces Intestinal Inflammation in Zebrafish Larvae. En: PLoS ONE. 2013 ; Vol. 8, N.º 7.
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Soybean Meal Induces Intestinal Inflammation in Zebrafish Larvae. / Hedrera, Manuel I.; Galdames, Jorge A.; Jimenez-Reyes, Maria F.; Reyes, Ariel E.; Avendaño-Herrera, Ruben; Romero, Jaime; Feijóo, Carmen G.

En: PLoS ONE, Vol. 8, N.º 7, e69983, 23.07.2013.

Resultado de la investigación: Article

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