Resumen
OmpW of Salmonella enterica serovar Typhimurium has been described as a minor porin involved in osmoregulation, and is also affected by environmental conditions. Biochemical and genetic evidence from our laboratory indicates that OmpW is involved in efflux of and resistance towards paraquat (PQ), and its expression has been shown to be activated in response to oxidative stress. In this study we have explored ompW expression in response to PQ. Primer extension and transcriptional fusions showed that its expression was induced in the presence of PQ. In silico analyses suggested a putative binding site for the SoxS transcriptional factor at the ompW regulatory region. Electrophoretic mobility shift assays (EMSAs) and footprinting experiments showed that SoxS binds at a region that starts close to -54 and ends at about -197 upstream of the transcription start site. Transcriptional fusions support the relevance of this region in ompW activation. The SoxS site is in the forward orientation and its location suggests that the ompW gene has a class I SoxS-dependent promoter.
| Idioma original | English |
|---|---|
| Páginas (desde-hasta) | 2490-2497 |
| Número de páginas | 8 |
| Publicación | Microbiology |
| Volumen | 155 |
| N.º | 8 |
| DOI | |
| Estado | Published - 2009 |
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ASJC Scopus subject areas
- Microbiology
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SoxS regulates the expression of the Salmonella enterica serovar Typhimurium ompW gene. / Gil, F.; Hernández-Lucas, I.; Polanco, R.; Pacheco, N.; Collao, B.; Villareal, J. M.; Nardocci, G.; Calva, G.; Saavedra, C. P.
En: Microbiology, Vol. 155, N.º 8, 2009, p. 2490-2497.Resultado de la investigación: Article
TY - JOUR
T1 - SoxS regulates the expression of the Salmonella enterica serovar Typhimurium ompW gene
AU - Gil, F.
AU - Hernández-Lucas, I.
AU - Polanco, R.
AU - Pacheco, N.
AU - Collao, B.
AU - Villareal, J. M.
AU - Nardocci, G.
AU - Calva, G.
AU - Saavedra, C. P.
PY - 2009
Y1 - 2009
N2 - OmpW of Salmonella enterica serovar Typhimurium has been described as a minor porin involved in osmoregulation, and is also affected by environmental conditions. Biochemical and genetic evidence from our laboratory indicates that OmpW is involved in efflux of and resistance towards paraquat (PQ), and its expression has been shown to be activated in response to oxidative stress. In this study we have explored ompW expression in response to PQ. Primer extension and transcriptional fusions showed that its expression was induced in the presence of PQ. In silico analyses suggested a putative binding site for the SoxS transcriptional factor at the ompW regulatory region. Electrophoretic mobility shift assays (EMSAs) and footprinting experiments showed that SoxS binds at a region that starts close to -54 and ends at about -197 upstream of the transcription start site. Transcriptional fusions support the relevance of this region in ompW activation. The SoxS site is in the forward orientation and its location suggests that the ompW gene has a class I SoxS-dependent promoter.
AB - OmpW of Salmonella enterica serovar Typhimurium has been described as a minor porin involved in osmoregulation, and is also affected by environmental conditions. Biochemical and genetic evidence from our laboratory indicates that OmpW is involved in efflux of and resistance towards paraquat (PQ), and its expression has been shown to be activated in response to oxidative stress. In this study we have explored ompW expression in response to PQ. Primer extension and transcriptional fusions showed that its expression was induced in the presence of PQ. In silico analyses suggested a putative binding site for the SoxS transcriptional factor at the ompW regulatory region. Electrophoretic mobility shift assays (EMSAs) and footprinting experiments showed that SoxS binds at a region that starts close to -54 and ends at about -197 upstream of the transcription start site. Transcriptional fusions support the relevance of this region in ompW activation. The SoxS site is in the forward orientation and its location suggests that the ompW gene has a class I SoxS-dependent promoter.
UR - http://www.scopus.com/inward/record.url?scp=69949131704&partnerID=8YFLogxK
U2 - 10.1099/mic.0.027433-0
DO - 10.1099/mic.0.027433-0
M3 - Article
C2 - 19460824
AN - SCOPUS:69949131704
VL - 155
SP - 2490
EP - 2497
JO - Microbiology (United Kingdom)
JF - Microbiology (United Kingdom)
SN - 1350-0872
IS - 8
ER -