SoxS regulates the expression of the Salmonella enterica serovar Typhimurium ompW gene

F. Gil, I. Hernández-Lucas, R. Polanco, N. Pacheco, B. Collao, J. M. Villareal, G. Nardocci, G. Calva, C. P. Saavedra

Resultado de la investigación: Article

21 Citas (Scopus)

Resumen

OmpW of Salmonella enterica serovar Typhimurium has been described as a minor porin involved in osmoregulation, and is also affected by environmental conditions. Biochemical and genetic evidence from our laboratory indicates that OmpW is involved in efflux of and resistance towards paraquat (PQ), and its expression has been shown to be activated in response to oxidative stress. In this study we have explored ompW expression in response to PQ. Primer extension and transcriptional fusions showed that its expression was induced in the presence of PQ. In silico analyses suggested a putative binding site for the SoxS transcriptional factor at the ompW regulatory region. Electrophoretic mobility shift assays (EMSAs) and footprinting experiments showed that SoxS binds at a region that starts close to -54 and ends at about -197 upstream of the transcription start site. Transcriptional fusions support the relevance of this region in ompW activation. The SoxS site is in the forward orientation and its location suggests that the ompW gene has a class I SoxS-dependent promoter.

Idioma originalEnglish
Páginas (desde-hasta)2490-2497
Número de páginas8
PublicaciónMicrobiology
Volumen155
N.º8
DOI
EstadoPublished - 2009

Huella dactilar

Paraquat
Salmonella enterica
Genes
Osmoregulation
Porins
Nucleic Acid Regulatory Sequences
Transcription Initiation Site
Electrophoretic Mobility Shift Assay
Computer Simulation
Molecular Biology
Oxidative Stress
Binding Sites
Serogroup

ASJC Scopus subject areas

  • Microbiology

Citar esto

Gil, F. ; Hernández-Lucas, I. ; Polanco, R. ; Pacheco, N. ; Collao, B. ; Villareal, J. M. ; Nardocci, G. ; Calva, G. ; Saavedra, C. P. / SoxS regulates the expression of the Salmonella enterica serovar Typhimurium ompW gene. En: Microbiology. 2009 ; Vol. 155, N.º 8. pp. 2490-2497.
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abstract = "OmpW of Salmonella enterica serovar Typhimurium has been described as a minor porin involved in osmoregulation, and is also affected by environmental conditions. Biochemical and genetic evidence from our laboratory indicates that OmpW is involved in efflux of and resistance towards paraquat (PQ), and its expression has been shown to be activated in response to oxidative stress. In this study we have explored ompW expression in response to PQ. Primer extension and transcriptional fusions showed that its expression was induced in the presence of PQ. In silico analyses suggested a putative binding site for the SoxS transcriptional factor at the ompW regulatory region. Electrophoretic mobility shift assays (EMSAs) and footprinting experiments showed that SoxS binds at a region that starts close to -54 and ends at about -197 upstream of the transcription start site. Transcriptional fusions support the relevance of this region in ompW activation. The SoxS site is in the forward orientation and its location suggests that the ompW gene has a class I SoxS-dependent promoter.",
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Gil, F, Hernández-Lucas, I, Polanco, R, Pacheco, N, Collao, B, Villareal, JM, Nardocci, G, Calva, G & Saavedra, CP 2009, 'SoxS regulates the expression of the Salmonella enterica serovar Typhimurium ompW gene', Microbiology, vol. 155, n.º 8, pp. 2490-2497. https://doi.org/10.1099/mic.0.027433-0

SoxS regulates the expression of the Salmonella enterica serovar Typhimurium ompW gene. / Gil, F.; Hernández-Lucas, I.; Polanco, R.; Pacheco, N.; Collao, B.; Villareal, J. M.; Nardocci, G.; Calva, G.; Saavedra, C. P.

En: Microbiology, Vol. 155, N.º 8, 2009, p. 2490-2497.

Resultado de la investigación: Article

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AU - Gil, F.

AU - Hernández-Lucas, I.

AU - Polanco, R.

AU - Pacheco, N.

AU - Collao, B.

AU - Villareal, J. M.

AU - Nardocci, G.

AU - Calva, G.

AU - Saavedra, C. P.

PY - 2009

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AB - OmpW of Salmonella enterica serovar Typhimurium has been described as a minor porin involved in osmoregulation, and is also affected by environmental conditions. Biochemical and genetic evidence from our laboratory indicates that OmpW is involved in efflux of and resistance towards paraquat (PQ), and its expression has been shown to be activated in response to oxidative stress. In this study we have explored ompW expression in response to PQ. Primer extension and transcriptional fusions showed that its expression was induced in the presence of PQ. In silico analyses suggested a putative binding site for the SoxS transcriptional factor at the ompW regulatory region. Electrophoretic mobility shift assays (EMSAs) and footprinting experiments showed that SoxS binds at a region that starts close to -54 and ends at about -197 upstream of the transcription start site. Transcriptional fusions support the relevance of this region in ompW activation. The SoxS site is in the forward orientation and its location suggests that the ompW gene has a class I SoxS-dependent promoter.

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