Ski Is Required for Tri-Methylation of H3K9 in Major Satellite and for Repression of Pericentromeric Genes: Mmp3, Mmp10 and Mmp13, in Mouse Fibroblasts

Claudio Cappelli, Hugo Sepulveda, Solange Rivas, Víctor Pola, Ulises Urzúa, Gerardo Donoso, Eduardo Sagredo, David Carrero, Emmanuel Casanova-Ortiz, Alfredo Sagredo, Marisel González, Marcia Manterola, Gino Nardocci, Ricardo Armisén, Martin Montecino, Katherine Marcelain

Resultado de la investigación: Contribución a una revistaArtículo

Resumen

Several mechanisms directing a rapid transcriptional reactivation of genes immediately after mitosis have been described. However, little is known about the maintenance of repressive signals during mitosis. In this work, we address the role of Ski in the repression of gene expression during M/G1 transition in mouse embryonic fibroblasts (MEFs). We found that Ski localises as a distinct pair of dots at the pericentromeric region of mitotic chromosomes, and the absence of the protein is related to high acetylation and low tri-methylation of H3K9 in pericentromeric major satellite. Moreover, differential expression assays in early G1 cells showed that the presence of Ski is significantly associated with repression of genes localised nearby to pericentromeric DNA. In mitotic cells, chromatin immunoprecipitation assays confirmed the association of Ski to major satellite and the promoters of the most repressed genes: Mmp3, Mmp10 and Mmp13. These genes are at pericentromeric region of chromosome 9. In these promoters, the presence of Ski resulted in increased H3K9 tri-methylation levels. This Ski-dependent regulation is also observed during interphase. Consequently, Mmp activity is augmented in Ski −/− MEFs. Altogether, these data indicate that association of Ski with the pericentromeric region of chromosomes during mitosis is required to maintain the silencing bookmarks of underlying chromatin.

Idioma originalInglés
PublicaciónJournal of Molecular Biology
DOI
EstadoEn prensa - 1 ene 2020

Áreas temáticas de ASJC Scopus

  • Biología estructural
  • Biología molecular

Huella Profundice en los temas de investigación de 'Ski Is Required for Tri-Methylation of H3K9 in Major Satellite and for Repression of Pericentromeric Genes: Mmp3, Mmp10 and Mmp13, in Mouse Fibroblasts'. En conjunto forman una huella única.

  • Citar esto

    Cappelli, C., Sepulveda, H., Rivas, S., Pola, V., Urzúa, U., Donoso, G., Sagredo, E., Carrero, D., Casanova-Ortiz, E., Sagredo, A., González, M., Manterola, M., Nardocci, G., Armisén, R., Montecino, M., & Marcelain, K. (Aceptado/En prensa). Ski Is Required for Tri-Methylation of H3K9 in Major Satellite and for Repression of Pericentromeric Genes: Mmp3, Mmp10 and Mmp13, in Mouse Fibroblasts. Journal of Molecular Biology. https://doi.org/10.1016/j.jmb.2020.03.013