Singlet oxygen-promoted mitochondrial permeability transition pore (MTP) opening in rat liver mitochondria

J. Turin, A. M. Almeida, P. Di Mascio, A. E. Vercesi, I. L. Nantes -

Resultado de la investigación: Article

Resumen

Singlet molecular oxygen (O21Δg)like other reactive oxygen species(ROS), can be generated in mammafian cells during oxidative stress by an energy transfer mechanism following the production of triplet species during the course of lipid peroxidation. Furthermore, the photodynamic action of some drugs is mediated by singlet oxygen. In this regard, mitochondria are important targets of ROS-promoted cell damage and play a key role in diseases sensitive to photodynamic therapy. This work characterizes the effect of O21Δg generated by NDPO 2 on rat liver mitochondria. NDPO2 inhibits mitochondrial respiration at sites I and II but not at site IV. NDPO2 treated mitochondria exhibits loss of transmembrane potential (Δψ) in a dose and exposition timedependent manner. This Δψ decrease is sensitive to the protective effect of EGTA, ADP, CyA and DTT. Alterations in membrane proteins were determined by SDS-PAGE electrophoresis of solubilized membrane proteins in the presence of eosin-5-maleimide. The results evidenced thiol groups oxidation that was prevented by dithiothreitol (DTT)and ADP. NDPO2-promoted mitochondrial damage was increased in D20 medium and prevented by histidine. Through this work, we suggested that singlet oxygen-promoted damage in rat liver mitochondria is mediated by Ca2+-dependent mitochondrial permeability transition pore (MPT) opening as demonstrated by the protective effect of EGTA, ADP and CyA. Supported by FAPESP, CNPq, PADCT and FAEP.

Idioma originalEnglish
PublicaciónFASEB Journal
Volumen11
N.º9
EstadoPublished - 1997

Huella dactilar

Singlet Oxygen
Mitochondria
singlet oxygen
Liver Mitochondrion
Liver
Adenosine Diphosphate
Rats
permeability
mitochondria
Dithiothreitol
Egtazic Acid
liver
dithiothreitol
Reactive Oxygen Species
rats
Membrane Proteins
membrane proteins
protective effect
reactive oxygen species
Energy Transfer

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Biotechnology

Citar esto

Turin, J., Almeida, A. M., Di Mascio, P., Vercesi, A. E., & Nantes -, I. L. (1997). Singlet oxygen-promoted mitochondrial permeability transition pore (MTP) opening in rat liver mitochondria. FASEB Journal, 11(9).
Turin, J. ; Almeida, A. M. ; Di Mascio, P. ; Vercesi, A. E. ; Nantes -, I. L. / Singlet oxygen-promoted mitochondrial permeability transition pore (MTP) opening in rat liver mitochondria. En: FASEB Journal. 1997 ; Vol. 11, N.º 9.
@article{e916fa8d7585499cb4349c5d292579b4,
title = "Singlet oxygen-promoted mitochondrial permeability transition pore (MTP) opening in rat liver mitochondria",
abstract = "Singlet molecular oxygen (O21Δg)like other reactive oxygen species(ROS), can be generated in mammafian cells during oxidative stress by an energy transfer mechanism following the production of triplet species during the course of lipid peroxidation. Furthermore, the photodynamic action of some drugs is mediated by singlet oxygen. In this regard, mitochondria are important targets of ROS-promoted cell damage and play a key role in diseases sensitive to photodynamic therapy. This work characterizes the effect of O21Δg generated by NDPO 2 on rat liver mitochondria. NDPO2 inhibits mitochondrial respiration at sites I and II but not at site IV. NDPO2 treated mitochondria exhibits loss of transmembrane potential (Δψ) in a dose and exposition timedependent manner. This Δψ decrease is sensitive to the protective effect of EGTA, ADP, CyA and DTT. Alterations in membrane proteins were determined by SDS-PAGE electrophoresis of solubilized membrane proteins in the presence of eosin-5-maleimide. The results evidenced thiol groups oxidation that was prevented by dithiothreitol (DTT)and ADP. NDPO2-promoted mitochondrial damage was increased in D20 medium and prevented by histidine. Through this work, we suggested that singlet oxygen-promoted damage in rat liver mitochondria is mediated by Ca2+-dependent mitochondrial permeability transition pore (MPT) opening as demonstrated by the protective effect of EGTA, ADP and CyA. Supported by FAPESP, CNPq, PADCT and FAEP.",
author = "J. Turin and Almeida, {A. M.} and {Di Mascio}, P. and Vercesi, {A. E.} and {Nantes -}, {I. L.}",
year = "1997",
language = "English",
volume = "11",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "9",

}

Turin, J, Almeida, AM, Di Mascio, P, Vercesi, AE & Nantes -, IL 1997, 'Singlet oxygen-promoted mitochondrial permeability transition pore (MTP) opening in rat liver mitochondria', FASEB Journal, vol. 11, n.º 9.

Singlet oxygen-promoted mitochondrial permeability transition pore (MTP) opening in rat liver mitochondria. / Turin, J.; Almeida, A. M.; Di Mascio, P.; Vercesi, A. E.; Nantes -, I. L.

En: FASEB Journal, Vol. 11, N.º 9, 1997.

Resultado de la investigación: Article

TY - JOUR

T1 - Singlet oxygen-promoted mitochondrial permeability transition pore (MTP) opening in rat liver mitochondria

AU - Turin, J.

AU - Almeida, A. M.

AU - Di Mascio, P.

AU - Vercesi, A. E.

AU - Nantes -, I. L.

PY - 1997

Y1 - 1997

N2 - Singlet molecular oxygen (O21Δg)like other reactive oxygen species(ROS), can be generated in mammafian cells during oxidative stress by an energy transfer mechanism following the production of triplet species during the course of lipid peroxidation. Furthermore, the photodynamic action of some drugs is mediated by singlet oxygen. In this regard, mitochondria are important targets of ROS-promoted cell damage and play a key role in diseases sensitive to photodynamic therapy. This work characterizes the effect of O21Δg generated by NDPO 2 on rat liver mitochondria. NDPO2 inhibits mitochondrial respiration at sites I and II but not at site IV. NDPO2 treated mitochondria exhibits loss of transmembrane potential (Δψ) in a dose and exposition timedependent manner. This Δψ decrease is sensitive to the protective effect of EGTA, ADP, CyA and DTT. Alterations in membrane proteins were determined by SDS-PAGE electrophoresis of solubilized membrane proteins in the presence of eosin-5-maleimide. The results evidenced thiol groups oxidation that was prevented by dithiothreitol (DTT)and ADP. NDPO2-promoted mitochondrial damage was increased in D20 medium and prevented by histidine. Through this work, we suggested that singlet oxygen-promoted damage in rat liver mitochondria is mediated by Ca2+-dependent mitochondrial permeability transition pore (MPT) opening as demonstrated by the protective effect of EGTA, ADP and CyA. Supported by FAPESP, CNPq, PADCT and FAEP.

AB - Singlet molecular oxygen (O21Δg)like other reactive oxygen species(ROS), can be generated in mammafian cells during oxidative stress by an energy transfer mechanism following the production of triplet species during the course of lipid peroxidation. Furthermore, the photodynamic action of some drugs is mediated by singlet oxygen. In this regard, mitochondria are important targets of ROS-promoted cell damage and play a key role in diseases sensitive to photodynamic therapy. This work characterizes the effect of O21Δg generated by NDPO 2 on rat liver mitochondria. NDPO2 inhibits mitochondrial respiration at sites I and II but not at site IV. NDPO2 treated mitochondria exhibits loss of transmembrane potential (Δψ) in a dose and exposition timedependent manner. This Δψ decrease is sensitive to the protective effect of EGTA, ADP, CyA and DTT. Alterations in membrane proteins were determined by SDS-PAGE electrophoresis of solubilized membrane proteins in the presence of eosin-5-maleimide. The results evidenced thiol groups oxidation that was prevented by dithiothreitol (DTT)and ADP. NDPO2-promoted mitochondrial damage was increased in D20 medium and prevented by histidine. Through this work, we suggested that singlet oxygen-promoted damage in rat liver mitochondria is mediated by Ca2+-dependent mitochondrial permeability transition pore (MPT) opening as demonstrated by the protective effect of EGTA, ADP and CyA. Supported by FAPESP, CNPq, PADCT and FAEP.

UR - http://www.scopus.com/inward/record.url?scp=33750159105&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33750159105

VL - 11

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 9

ER -