Sequence Composition of Rat Ascites Chromosomal Ribonucleic Acid

David S. Holmes, James Bonner, John E. Mayfield

Resultado de la investigación: Article

15 Citas (Scopus)

Resumen

A population of chromatin-associated low molecular weight RNA molecules (cRNA) prepared from rat Novikoff ascites cells hybridizes to a minimum of 16% of isolated middle repetitive and a minimum of 1% of isolated single copy rat DNA as measured in solution. This sums to about 4.0% hybridization of total DNA. In solution the rate of hybridization of cRNA to isolated middle repetitive DNA is approximately that expected if 80-100% of the cRNA consists of middle repetitive transcripts. In contrast cRNA hybridizes to about 4.7 % of total rat DNA immobilized on filters at a rate at least 100 times slower than that predicted from the solution hybridization, suggesting that steric or geometric effects are considerable in this type of assay. In a solution hybridization reaction to total DNA, present in excess, at least 50% of the cRNA hybridizes at an average rate similar to the major component of the middle repetitive DNA. The Tm of cRNA, hybridized to filter-bound DNA, is at least 12° lower than native DNA. The lowering of Tm may be due, in part, to base-pair mismatch and, in part, to the shortness of the hybrids.

Idioma originalEnglish
Páginas (desde-hasta)849-855
Número de páginas7
PublicaciónBiochemistry
Volumen13
N.º5
DOI
EstadoPublished - 1 feb 1974

Huella dactilar

Complementary RNA
Ascites
Rats
RNA
DNA
Chemical analysis
Base Pair Mismatch
Immobilized Nucleic Acids
Chromatin
Assays
Molecular Weight
Molecular weight
Molecules
Population

ASJC Scopus subject areas

  • Biochemistry

Citar esto

Holmes, David S. ; Bonner, James ; Mayfield, John E. / Sequence Composition of Rat Ascites Chromosomal Ribonucleic Acid. En: Biochemistry. 1974 ; Vol. 13, N.º 5. pp. 849-855.
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Holmes, DS, Bonner, J & Mayfield, JE 1974, 'Sequence Composition of Rat Ascites Chromosomal Ribonucleic Acid', Biochemistry, vol. 13, n.º 5, pp. 849-855. https://doi.org/10.1021/bi00702a002

Sequence Composition of Rat Ascites Chromosomal Ribonucleic Acid. / Holmes, David S.; Bonner, James; Mayfield, John E.

En: Biochemistry, Vol. 13, N.º 5, 01.02.1974, p. 849-855.

Resultado de la investigación: Article

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