Resumen
The periplasmic blue copper protein rusticyanin is thought to play an important role in iron oxidation by Thiobacillus ferrooxidans. We present the sequence of the gene, rus, encoding rusticyanin together with about 1.4 kb of upstream and 0.3 kb of downstream DNA. The rus gene is unique to T. ferrooxidans. Evidence is presented that it is the last gene of an operon and that it can be transcribed from its own promoter. In ATCC33020 strain, rusticyanin is synthesized in ferrous iron but also in sulfur growth conditions suggesting that it could play a role in both energetic metabolisms. The rus gene transcribed from a vector promoter in Escherichia coli leads to the production of a processed aporusticyanin in the periplasmic space, indicating that its signal sequence is correctly recognized by the secretion machinery and the signal peptidase of E. coli.
| Idioma original | English |
|---|---|
| Páginas (desde-hasta) | 99-112 |
| Número de páginas | 14 |
| Publicación | Biochimica et Biophysica Acta - Gene Structure and Expression |
| Volumen | 1443 |
| N.º | 1-2 |
| DOI | |
| Estado | Published - 26 nov 1998 |
Huella dactilar
ASJC Scopus subject areas
- Structural Biology
- Biophysics
- Biochemistry
- Genetics
Citar esto
}
Sequence and expression of the rusticyanin structural gene from Thiobacillus ferrooxidans ATCC33020 strain. / Bengrine, Abderrahmane; Guiliani, Nicolas; Appia-Ayme, Corinne; Jedlicki, Eugenia; Holmes, David S.; Chippaux, Marc; Bonnefoy, V.
En: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1443, N.º 1-2, 26.11.1998, p. 99-112.Resultado de la investigación: Article
TY - JOUR
T1 - Sequence and expression of the rusticyanin structural gene from Thiobacillus ferrooxidans ATCC33020 strain
AU - Bengrine, Abderrahmane
AU - Guiliani, Nicolas
AU - Appia-Ayme, Corinne
AU - Jedlicki, Eugenia
AU - Holmes, David S.
AU - Chippaux, Marc
AU - Bonnefoy, V.
PY - 1998/11/26
Y1 - 1998/11/26
N2 - The periplasmic blue copper protein rusticyanin is thought to play an important role in iron oxidation by Thiobacillus ferrooxidans. We present the sequence of the gene, rus, encoding rusticyanin together with about 1.4 kb of upstream and 0.3 kb of downstream DNA. The rus gene is unique to T. ferrooxidans. Evidence is presented that it is the last gene of an operon and that it can be transcribed from its own promoter. In ATCC33020 strain, rusticyanin is synthesized in ferrous iron but also in sulfur growth conditions suggesting that it could play a role in both energetic metabolisms. The rus gene transcribed from a vector promoter in Escherichia coli leads to the production of a processed aporusticyanin in the periplasmic space, indicating that its signal sequence is correctly recognized by the secretion machinery and the signal peptidase of E. coli.
AB - The periplasmic blue copper protein rusticyanin is thought to play an important role in iron oxidation by Thiobacillus ferrooxidans. We present the sequence of the gene, rus, encoding rusticyanin together with about 1.4 kb of upstream and 0.3 kb of downstream DNA. The rus gene is unique to T. ferrooxidans. Evidence is presented that it is the last gene of an operon and that it can be transcribed from its own promoter. In ATCC33020 strain, rusticyanin is synthesized in ferrous iron but also in sulfur growth conditions suggesting that it could play a role in both energetic metabolisms. The rus gene transcribed from a vector promoter in Escherichia coli leads to the production of a processed aporusticyanin in the periplasmic space, indicating that its signal sequence is correctly recognized by the secretion machinery and the signal peptidase of E. coli.
KW - (Thiobacillus ferrooxidans)
KW - Copper protein
KW - Electron transport
KW - Iron oxidation
KW - Rusticyanin
UR - http://www.scopus.com/inward/record.url?scp=0032570018&partnerID=8YFLogxK
U2 - 10.1016/S0167-4781(98)00199-7
DO - 10.1016/S0167-4781(98)00199-7
M3 - Article
C2 - 9838063
AN - SCOPUS:0032570018
VL - 1443
SP - 99
EP - 112
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
SN - 0167-4781
IS - 1-2
ER -