Sequence and expression of the rusticyanin structural gene from Thiobacillus ferrooxidans ATCC33020 strain

Abderrahmane Bengrine, Nicolas Guiliani, Corinne Appia-Ayme, Eugenia Jedlicki, David S. Holmes, Marc Chippaux, V. Bonnefoy

Resultado de la investigación: Contribución a una revistaArtículo

35 Citas (Scopus)

Resumen

The periplasmic blue copper protein rusticyanin is thought to play an important role in iron oxidation by Thiobacillus ferrooxidans. We present the sequence of the gene, rus, encoding rusticyanin together with about 1.4 kb of upstream and 0.3 kb of downstream DNA. The rus gene is unique to T. ferrooxidans. Evidence is presented that it is the last gene of an operon and that it can be transcribed from its own promoter. In ATCC33020 strain, rusticyanin is synthesized in ferrous iron but also in sulfur growth conditions suggesting that it could play a role in both energetic metabolisms. The rus gene transcribed from a vector promoter in Escherichia coli leads to the production of a processed aporusticyanin in the periplasmic space, indicating that its signal sequence is correctly recognized by the secretion machinery and the signal peptidase of E. coli.

Idioma originalInglés
Páginas (desde-hasta)99-112
Número de páginas14
PublicaciónBiochimica et Biophysica Acta - Gene Structure and Expression
Volumen1443
N.º1-2
DOI
EstadoPublicada - 26 nov 1998

Áreas temáticas de ASJC Scopus

  • Biología estructural
  • Biofísica
  • Bioquímica
  • Genética

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