Sequence and expression of the rusticyanin structural gene from Thiobacillus ferrooxidans ATCC33020 strain

Abderrahmane Bengrine, Nicolas Guiliani, Corinne Appia-Ayme, Eugenia Jedlicki, David S. Holmes, Marc Chippaux, V. Bonnefoy

Resultado de la investigación: Article

34 Citas (Scopus)

Resumen

The periplasmic blue copper protein rusticyanin is thought to play an important role in iron oxidation by Thiobacillus ferrooxidans. We present the sequence of the gene, rus, encoding rusticyanin together with about 1.4 kb of upstream and 0.3 kb of downstream DNA. The rus gene is unique to T. ferrooxidans. Evidence is presented that it is the last gene of an operon and that it can be transcribed from its own promoter. In ATCC33020 strain, rusticyanin is synthesized in ferrous iron but also in sulfur growth conditions suggesting that it could play a role in both energetic metabolisms. The rus gene transcribed from a vector promoter in Escherichia coli leads to the production of a processed aporusticyanin in the periplasmic space, indicating that its signal sequence is correctly recognized by the secretion machinery and the signal peptidase of E. coli.

Idioma originalEnglish
Páginas (desde-hasta)99-112
Número de páginas14
PublicaciónBiochimica et Biophysica Acta - Gene Structure and Expression
Volumen1443
N.º1-2
DOI
EstadoPublished - 26 nov 1998

Huella dactilar

Thiobacillus
Genes
Escherichia coli
Iron
Gene encoding
Protein Sorting Signals
Sulfur
Metabolism
Periplasm
Machinery
Copper
Operon
Oxidation
DNA
rusticyanin
Proteins
Growth

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

Citar esto

Bengrine, Abderrahmane ; Guiliani, Nicolas ; Appia-Ayme, Corinne ; Jedlicki, Eugenia ; Holmes, David S. ; Chippaux, Marc ; Bonnefoy, V. / Sequence and expression of the rusticyanin structural gene from Thiobacillus ferrooxidans ATCC33020 strain. En: Biochimica et Biophysica Acta - Gene Structure and Expression. 1998 ; Vol. 1443, N.º 1-2. pp. 99-112.
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Sequence and expression of the rusticyanin structural gene from Thiobacillus ferrooxidans ATCC33020 strain. / Bengrine, Abderrahmane; Guiliani, Nicolas; Appia-Ayme, Corinne; Jedlicki, Eugenia; Holmes, David S.; Chippaux, Marc; Bonnefoy, V.

En: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1443, N.º 1-2, 26.11.1998, p. 99-112.

Resultado de la investigación: Article

TY - JOUR

T1 - Sequence and expression of the rusticyanin structural gene from Thiobacillus ferrooxidans ATCC33020 strain

AU - Bengrine, Abderrahmane

AU - Guiliani, Nicolas

AU - Appia-Ayme, Corinne

AU - Jedlicki, Eugenia

AU - Holmes, David S.

AU - Chippaux, Marc

AU - Bonnefoy, V.

PY - 1998/11/26

Y1 - 1998/11/26

N2 - The periplasmic blue copper protein rusticyanin is thought to play an important role in iron oxidation by Thiobacillus ferrooxidans. We present the sequence of the gene, rus, encoding rusticyanin together with about 1.4 kb of upstream and 0.3 kb of downstream DNA. The rus gene is unique to T. ferrooxidans. Evidence is presented that it is the last gene of an operon and that it can be transcribed from its own promoter. In ATCC33020 strain, rusticyanin is synthesized in ferrous iron but also in sulfur growth conditions suggesting that it could play a role in both energetic metabolisms. The rus gene transcribed from a vector promoter in Escherichia coli leads to the production of a processed aporusticyanin in the periplasmic space, indicating that its signal sequence is correctly recognized by the secretion machinery and the signal peptidase of E. coli.

AB - The periplasmic blue copper protein rusticyanin is thought to play an important role in iron oxidation by Thiobacillus ferrooxidans. We present the sequence of the gene, rus, encoding rusticyanin together with about 1.4 kb of upstream and 0.3 kb of downstream DNA. The rus gene is unique to T. ferrooxidans. Evidence is presented that it is the last gene of an operon and that it can be transcribed from its own promoter. In ATCC33020 strain, rusticyanin is synthesized in ferrous iron but also in sulfur growth conditions suggesting that it could play a role in both energetic metabolisms. The rus gene transcribed from a vector promoter in Escherichia coli leads to the production of a processed aporusticyanin in the periplasmic space, indicating that its signal sequence is correctly recognized by the secretion machinery and the signal peptidase of E. coli.

KW - (Thiobacillus ferrooxidans)

KW - Copper protein

KW - Electron transport

KW - Iron oxidation

KW - Rusticyanin

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