Sensitivity to potassium tellurite of Escherichia coli cells deficient in CSD, CsdB and IscS cysteine desulfurases

Diana M. Rojas, Claudio C. Vásquez

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

15 Citas (Scopus)

Resumen

The csdA, csdB and iscS genes encoding for cysteine desulfurase enzymatic activities in Escherichia coli were independently inactivated and potassium tellurite sensitivity, determined for each of the resulting mutant clones, was found to be iscS > csdB > csdA. Structural genes encoding for each of the wild-type cysteine desulfurases were cloned into a vector containing the regulated ara promoter and further introduced into the mutant strains. Desulfurase-deficient cells transformed with homolog or paralog desulfurase genes and grown in arabinose-amended media restored their basal tellurite resistance. While csdB gene complemented the auxotrophy of csdB and iscS mutants for nicotinic acid, the iscS gene only complemented the auxotrophy of iscS cells for thiamine. Introduction of the csdA gene into the desulfurase-deficient strains did not change tellurite resistance or nutritional requirement patterns of the recipient cells. Complementation analysis could not be performed under anaerobic conditions because the three mutants did not show tellurite hypersensitivity. These results indicate that oxidative stress is involved in tellurite toxicity in E. coli.

Idioma originalInglés
Páginas (desde-hasta)465-471
Número de páginas7
PublicaciónResearch in Microbiology
Volumen156
N.º4
DOI
EstadoPublicada - may 2005
Publicado de forma externa

Áreas temáticas de ASJC Scopus

  • Microbiología
  • Biología molecular

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