Selective stimulation of intrinsic excitatory and inhibitory motor pathways in porcine lower oesophageal sphincter

B. Lecea, E. Martínez, M. Aulí, Á Opazo, P. Clavé

Resultado de la investigación: Article

4 Citas (Scopus)

Resumen

The mechanisms of stimulation of inhibitory and excitatory motor neurons (MNs) in the lower oesophageal sphincter (LOS) are not fully understood. The aim of this study was to assess the effect of selective stimulation of inhibitory and excitatory MNs in porcine LOS through nicotinic acetylcholine receptors (nAChRs), 5-HT3 and P2X receptors. Circular LOS strips from adult pigs were studied in organ baths. We compared the effects of stimulation of MNs by electrical field stimulation (26 V, 0.3-20 Hz); nicotine (1-300 μmol L-1); 5-HT and 2-Me-5-HT (1 nmol-1-30 μmol L -1); and α,β-methylene ATP (α,β-meATP 1-100 μmol L-1); in standard Krebs solution; a non-adrenergic non-nitrergic non-purinergic (NANNNP) solution; and a non-adrenergic non-cholinergic (NANC) solution. Electrical stimulation of inhibitory MNs caused an intense LOS relaxation (-78.94 ± 4.50% of LOS tone); and of excitatory MNs, a strong contraction (17.89 ± 1.96 g). Nicotine 100 μmol L-1 relaxed LOS (-84.67 ± 3.98%) in standard Krebs solution, an effect reduced by Tetrodotoxin (TTX) 1 μmol L-1. Nicotine induced a weak TTX-sensitive contraction (1.64 ± 0.4 g) in NANNNP solution. 5-HT 10 μmol L-1 and 2-Me-5-HT 30 μmol L -1 contracted LOS in standard, NANC and NANNNP conditions, maximal responses (7.30 ± 1.52 g, 3.50 ± 0.18 g respectively) being reduced by TTX. α,β-meATP 100 μmol L-1 caused a LOS relaxation (-17.45 ± 6.62%) unaffected by TTX in NANC solution, and a contraction (6.7 ± 0.85 g) antagonized by TTX in NANNNP solution. Our results suggest selective mechanisms for stimulation of intrinsic excitatory and inhibitory motor pathways in porcine LOS. Inhibitory MNs are strongly stimulated by nAChRs and do not respond to stimulation of 5-HT3 and P2X receptors. By contrast, excitatory MNs are stimulated through 5-HT 3 and P2X receptors, stimulation through nACRs being difficult and causing a weak response.

Idioma originalEnglish
PublicaciónNeurogastroenterology and Motility
Volumen21
N.º12
DOI
EstadoPublished - 1 dic 2009

Huella dactilar

Efferent Pathways
Lower Esophageal Sphincter
Motor Neurons
Swine
Tetrodotoxin
Serotonin
Nicotine
Receptors, Serotonin, 5-HT3
Nicotinic Receptors
Electric Stimulation
Baths
Adenosine Triphosphate

ASJC Scopus subject areas

  • Physiology
  • Endocrine and Autonomic Systems
  • Gastroenterology

Citar esto

@article{f3032f4e4fef45d9bb054c4abb817111,
title = "Selective stimulation of intrinsic excitatory and inhibitory motor pathways in porcine lower oesophageal sphincter",
abstract = "The mechanisms of stimulation of inhibitory and excitatory motor neurons (MNs) in the lower oesophageal sphincter (LOS) are not fully understood. The aim of this study was to assess the effect of selective stimulation of inhibitory and excitatory MNs in porcine LOS through nicotinic acetylcholine receptors (nAChRs), 5-HT3 and P2X receptors. Circular LOS strips from adult pigs were studied in organ baths. We compared the effects of stimulation of MNs by electrical field stimulation (26 V, 0.3-20 Hz); nicotine (1-300 μmol L-1); 5-HT and 2-Me-5-HT (1 nmol-1-30 μmol L -1); and α,β-methylene ATP (α,β-meATP 1-100 μmol L-1); in standard Krebs solution; a non-adrenergic non-nitrergic non-purinergic (NANNNP) solution; and a non-adrenergic non-cholinergic (NANC) solution. Electrical stimulation of inhibitory MNs caused an intense LOS relaxation (-78.94 ± 4.50{\%} of LOS tone); and of excitatory MNs, a strong contraction (17.89 ± 1.96 g). Nicotine 100 μmol L-1 relaxed LOS (-84.67 ± 3.98{\%}) in standard Krebs solution, an effect reduced by Tetrodotoxin (TTX) 1 μmol L-1. Nicotine induced a weak TTX-sensitive contraction (1.64 ± 0.4 g) in NANNNP solution. 5-HT 10 μmol L-1 and 2-Me-5-HT 30 μmol L -1 contracted LOS in standard, NANC and NANNNP conditions, maximal responses (7.30 ± 1.52 g, 3.50 ± 0.18 g respectively) being reduced by TTX. α,β-meATP 100 μmol L-1 caused a LOS relaxation (-17.45 ± 6.62{\%}) unaffected by TTX in NANC solution, and a contraction (6.7 ± 0.85 g) antagonized by TTX in NANNNP solution. Our results suggest selective mechanisms for stimulation of intrinsic excitatory and inhibitory motor pathways in porcine LOS. Inhibitory MNs are strongly stimulated by nAChRs and do not respond to stimulation of 5-HT3 and P2X receptors. By contrast, excitatory MNs are stimulated through 5-HT 3 and P2X receptors, stimulation through nACRs being difficult and causing a weak response.",
keywords = "Excitatory motor neurons, Inhibitory motor neurons, Ligand-gated ion channels, Lower oesophageal sphincter, Selective stimulation",
author = "B. Lecea and E. Mart{\'i}nez and M. Aul{\'i} and {\'A} Opazo and P. Clav{\'e}",
year = "2009",
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doi = "10.1111/j.1365-2982.2009.01357.x",
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journal = "Neurogastroenterology and Motility",
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Selective stimulation of intrinsic excitatory and inhibitory motor pathways in porcine lower oesophageal sphincter. / Lecea, B.; Martínez, E.; Aulí, M.; Opazo, Á; Clavé, P.

En: Neurogastroenterology and Motility, Vol. 21, N.º 12, 01.12.2009.

Resultado de la investigación: Article

TY - JOUR

T1 - Selective stimulation of intrinsic excitatory and inhibitory motor pathways in porcine lower oesophageal sphincter

AU - Lecea, B.

AU - Martínez, E.

AU - Aulí, M.

AU - Opazo, Á

AU - Clavé, P.

PY - 2009/12/1

Y1 - 2009/12/1

N2 - The mechanisms of stimulation of inhibitory and excitatory motor neurons (MNs) in the lower oesophageal sphincter (LOS) are not fully understood. The aim of this study was to assess the effect of selective stimulation of inhibitory and excitatory MNs in porcine LOS through nicotinic acetylcholine receptors (nAChRs), 5-HT3 and P2X receptors. Circular LOS strips from adult pigs were studied in organ baths. We compared the effects of stimulation of MNs by electrical field stimulation (26 V, 0.3-20 Hz); nicotine (1-300 μmol L-1); 5-HT and 2-Me-5-HT (1 nmol-1-30 μmol L -1); and α,β-methylene ATP (α,β-meATP 1-100 μmol L-1); in standard Krebs solution; a non-adrenergic non-nitrergic non-purinergic (NANNNP) solution; and a non-adrenergic non-cholinergic (NANC) solution. Electrical stimulation of inhibitory MNs caused an intense LOS relaxation (-78.94 ± 4.50% of LOS tone); and of excitatory MNs, a strong contraction (17.89 ± 1.96 g). Nicotine 100 μmol L-1 relaxed LOS (-84.67 ± 3.98%) in standard Krebs solution, an effect reduced by Tetrodotoxin (TTX) 1 μmol L-1. Nicotine induced a weak TTX-sensitive contraction (1.64 ± 0.4 g) in NANNNP solution. 5-HT 10 μmol L-1 and 2-Me-5-HT 30 μmol L -1 contracted LOS in standard, NANC and NANNNP conditions, maximal responses (7.30 ± 1.52 g, 3.50 ± 0.18 g respectively) being reduced by TTX. α,β-meATP 100 μmol L-1 caused a LOS relaxation (-17.45 ± 6.62%) unaffected by TTX in NANC solution, and a contraction (6.7 ± 0.85 g) antagonized by TTX in NANNNP solution. Our results suggest selective mechanisms for stimulation of intrinsic excitatory and inhibitory motor pathways in porcine LOS. Inhibitory MNs are strongly stimulated by nAChRs and do not respond to stimulation of 5-HT3 and P2X receptors. By contrast, excitatory MNs are stimulated through 5-HT 3 and P2X receptors, stimulation through nACRs being difficult and causing a weak response.

AB - The mechanisms of stimulation of inhibitory and excitatory motor neurons (MNs) in the lower oesophageal sphincter (LOS) are not fully understood. The aim of this study was to assess the effect of selective stimulation of inhibitory and excitatory MNs in porcine LOS through nicotinic acetylcholine receptors (nAChRs), 5-HT3 and P2X receptors. Circular LOS strips from adult pigs were studied in organ baths. We compared the effects of stimulation of MNs by electrical field stimulation (26 V, 0.3-20 Hz); nicotine (1-300 μmol L-1); 5-HT and 2-Me-5-HT (1 nmol-1-30 μmol L -1); and α,β-methylene ATP (α,β-meATP 1-100 μmol L-1); in standard Krebs solution; a non-adrenergic non-nitrergic non-purinergic (NANNNP) solution; and a non-adrenergic non-cholinergic (NANC) solution. Electrical stimulation of inhibitory MNs caused an intense LOS relaxation (-78.94 ± 4.50% of LOS tone); and of excitatory MNs, a strong contraction (17.89 ± 1.96 g). Nicotine 100 μmol L-1 relaxed LOS (-84.67 ± 3.98%) in standard Krebs solution, an effect reduced by Tetrodotoxin (TTX) 1 μmol L-1. Nicotine induced a weak TTX-sensitive contraction (1.64 ± 0.4 g) in NANNNP solution. 5-HT 10 μmol L-1 and 2-Me-5-HT 30 μmol L -1 contracted LOS in standard, NANC and NANNNP conditions, maximal responses (7.30 ± 1.52 g, 3.50 ± 0.18 g respectively) being reduced by TTX. α,β-meATP 100 μmol L-1 caused a LOS relaxation (-17.45 ± 6.62%) unaffected by TTX in NANC solution, and a contraction (6.7 ± 0.85 g) antagonized by TTX in NANNNP solution. Our results suggest selective mechanisms for stimulation of intrinsic excitatory and inhibitory motor pathways in porcine LOS. Inhibitory MNs are strongly stimulated by nAChRs and do not respond to stimulation of 5-HT3 and P2X receptors. By contrast, excitatory MNs are stimulated through 5-HT 3 and P2X receptors, stimulation through nACRs being difficult and causing a weak response.

KW - Excitatory motor neurons

KW - Inhibitory motor neurons

KW - Ligand-gated ion channels

KW - Lower oesophageal sphincter

KW - Selective stimulation

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U2 - 10.1111/j.1365-2982.2009.01357.x

DO - 10.1111/j.1365-2982.2009.01357.x

M3 - Article

C2 - 19614864

AN - SCOPUS:72449162172

VL - 21

JO - Neurogastroenterology and Motility

JF - Neurogastroenterology and Motility

SN - 1350-1925

IS - 12

ER -