Secretion of endoxylanase A from Penicillium purpurogenum by Saccharomyces cerevisiae transformed with genomic fungal DNA

Renato Chávez, Claudio Navarro, Inés Calderón, Alessandra Peirano, Paulina Bull, Jaime Eyzaguirre

Resultado de la investigación: Article

7 Citas (Scopus)

Resumen

Saccharomyces cerevisiae was transformed with a genomic library from Penicillium purpurogenum, and an endoxylanase-producing yeast clone (named 44A) that grows on xylose or xylan as sole carbon source was isolated. This yeast synthesizes xynA mRNA and secretes endoxylanase A to culture media when grown on xylan or xylose, but not glucose. Analysis by pulse-field gel electrophoresis and sequencing indicates that xynA, including its eight introns, has been inserted into the yeast genome. It was shown by sequencing that clone 44A is able to correctly splice xynA introns. This is the first successful attempt to express a fungal endoxylanase gene in yeast with correct intron splicing.

Idioma originalEnglish
Páginas (desde-hasta)237-241
Número de páginas5
PublicaciónFEMS Microbiology Letters
Volumen212
N.º2
DOI
EstadoPublished - 2 jul 2002

Huella dactilar

Fungal DNA
Endo-1,4-beta Xylanases
Penicillium
Saccharomyces cerevisiae
Yeasts
Introns
Xylans
Clone Cells
Fungal Genes
Genomic Library
Xylose
Culture Media
Electrophoresis
Carbon
Gels
Genome
Messenger RNA

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Citar esto

Chávez, Renato ; Navarro, Claudio ; Calderón, Inés ; Peirano, Alessandra ; Bull, Paulina ; Eyzaguirre, Jaime. / Secretion of endoxylanase A from Penicillium purpurogenum by Saccharomyces cerevisiae transformed with genomic fungal DNA. En: FEMS Microbiology Letters. 2002 ; Vol. 212, N.º 2. pp. 237-241.
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abstract = "Saccharomyces cerevisiae was transformed with a genomic library from Penicillium purpurogenum, and an endoxylanase-producing yeast clone (named 44A) that grows on xylose or xylan as sole carbon source was isolated. This yeast synthesizes xynA mRNA and secretes endoxylanase A to culture media when grown on xylan or xylose, but not glucose. Analysis by pulse-field gel electrophoresis and sequencing indicates that xynA, including its eight introns, has been inserted into the yeast genome. It was shown by sequencing that clone 44A is able to correctly splice xynA introns. This is the first successful attempt to express a fungal endoxylanase gene in yeast with correct intron splicing.",
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Secretion of endoxylanase A from Penicillium purpurogenum by Saccharomyces cerevisiae transformed with genomic fungal DNA. / Chávez, Renato; Navarro, Claudio; Calderón, Inés; Peirano, Alessandra; Bull, Paulina; Eyzaguirre, Jaime.

En: FEMS Microbiology Letters, Vol. 212, N.º 2, 02.07.2002, p. 237-241.

Resultado de la investigación: Article

TY - JOUR

T1 - Secretion of endoxylanase A from Penicillium purpurogenum by Saccharomyces cerevisiae transformed with genomic fungal DNA

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AU - Navarro, Claudio

AU - Calderón, Inés

AU - Peirano, Alessandra

AU - Bull, Paulina

AU - Eyzaguirre, Jaime

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KW - Heterologous gene expression

KW - Penicillium purpurogenum

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