Resumen
Saccharomyces cerevisiae was transformed with a genomic library from Penicillium purpurogenum, and an endoxylanase-producing yeast clone (named 44A) that grows on xylose or xylan as sole carbon source was isolated. This yeast synthesizes xynA mRNA and secretes endoxylanase A to culture media when grown on xylan or xylose, but not glucose. Analysis by pulse-field gel electrophoresis and sequencing indicates that xynA, including its eight introns, has been inserted into the yeast genome. It was shown by sequencing that clone 44A is able to correctly splice xynA introns. This is the first successful attempt to express a fungal endoxylanase gene in yeast with correct intron splicing.
| Idioma original | English |
|---|---|
| Páginas (desde-hasta) | 237-241 |
| Número de páginas | 5 |
| Publicación | FEMS Microbiology Letters |
| Volumen | 212 |
| N.º | 2 |
| DOI | |
| Estado | Published - 2 jul 2002 |
Huella dactilar
ASJC Scopus subject areas
- Microbiology
- Molecular Biology
- Genetics
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Secretion of endoxylanase A from Penicillium purpurogenum by Saccharomyces cerevisiae transformed with genomic fungal DNA. / Chávez, Renato; Navarro, Claudio; Calderón, Inés; Peirano, Alessandra; Bull, Paulina; Eyzaguirre, Jaime.
En: FEMS Microbiology Letters, Vol. 212, N.º 2, 02.07.2002, p. 237-241.Resultado de la investigación: Article
TY - JOUR
T1 - Secretion of endoxylanase A from Penicillium purpurogenum by Saccharomyces cerevisiae transformed with genomic fungal DNA
AU - Chávez, Renato
AU - Navarro, Claudio
AU - Calderón, Inés
AU - Peirano, Alessandra
AU - Bull, Paulina
AU - Eyzaguirre, Jaime
PY - 2002/7/2
Y1 - 2002/7/2
N2 - Saccharomyces cerevisiae was transformed with a genomic library from Penicillium purpurogenum, and an endoxylanase-producing yeast clone (named 44A) that grows on xylose or xylan as sole carbon source was isolated. This yeast synthesizes xynA mRNA and secretes endoxylanase A to culture media when grown on xylan or xylose, but not glucose. Analysis by pulse-field gel electrophoresis and sequencing indicates that xynA, including its eight introns, has been inserted into the yeast genome. It was shown by sequencing that clone 44A is able to correctly splice xynA introns. This is the first successful attempt to express a fungal endoxylanase gene in yeast with correct intron splicing.
AB - Saccharomyces cerevisiae was transformed with a genomic library from Penicillium purpurogenum, and an endoxylanase-producing yeast clone (named 44A) that grows on xylose or xylan as sole carbon source was isolated. This yeast synthesizes xynA mRNA and secretes endoxylanase A to culture media when grown on xylan or xylose, but not glucose. Analysis by pulse-field gel electrophoresis and sequencing indicates that xynA, including its eight introns, has been inserted into the yeast genome. It was shown by sequencing that clone 44A is able to correctly splice xynA introns. This is the first successful attempt to express a fungal endoxylanase gene in yeast with correct intron splicing.
KW - Heterologous gene expression
KW - Penicillium purpurogenum
KW - Saccharomyces cerevisiae
KW - Xylanases
UR - http://www.scopus.com/inward/record.url?scp=0037008127&partnerID=8YFLogxK
U2 - 10.1016/S0378-1097(02)00750-4
DO - 10.1016/S0378-1097(02)00750-4
M3 - Article
C2 - 12113940
AN - SCOPUS:0037008127
VL - 212
SP - 237
EP - 241
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 2
ER -