Secondary metabolite profiling of species of the genus usnea by UHPLC-ESI-OT-MS-MS

Francisco Salgado, Laura Albornoz, Carmen Cortéz, Elena Stashenko, Kelly Urrea-Vallejo, Edgar Nagles, Cesar Galicia-Virviescas, Alberto Cornejo, Alejandro Ardiles, Mario Simirgiotis, Olimpo García-Beltrán, Carlos Areche

Resultado de la investigación: Article

8 Citas (Scopus)

Resumen

Lichens are symbiotic associations of fungi with microalgae and/or cyanobacteria, which are considered among the slowest growing organisms, with strong tolerance to adverse environmental conditions. There are about 400 genera and 1600 species of lichens and those belonging to the Usnea genus comprise about 360 of these species. Usnea lichens have been used since ancient times as dyes, cosmetics, preservatives, deodorants and folk medicines. The phytochemistry of the Usnea genus includes more than 60 compounds which belong to the following classes: depsides, depsidones, depsones, lactones, quinones, phenolics, polysaccharides, fatty acids and dibenzofurans. Due to scarce knowledge of metabolomic profiles of Usnea species (U. barbata, U. antarctica, U. rubicunda and U. subfloridana), a study based on UHPLC-ESI-OT-MS-MS was performed for a comprehensive characterization of their secondary metabolites. From the methanolic extracts of these species a total of 73 metabolites were identified for the first time using this hyphenated technique, including 34 compounds in U. barbata, 21 in U. antarctica, 38 in U. rubicunda and 37 in U. subfloridana. Besides, a total of 13 metabolites were not identified and reported so far, and could be new according to our data analysis. This study showed that this hyphenated technique is rapid, effective and accurate for phytochemical identification of lichen metabolites and the data collected could be useful for chemotaxonomic studies.

Idioma originalEnglish
Número de artículo54
PublicaciónMolecules
Volumen23
N.º1
DOI
EstadoPublished - 1 ene 2018

Huella dactilar

Usnea
lichens
Lichens
metabolites
Metabolites
Antarctic regions
Depsides
Deodorants
preservatives
Microalgae
Quinones
Metabolomics
fungi
Cosmetics
polysaccharides
Phytochemicals
Cyanobacteria
quinones
Traditional Medicine
Lactones

ASJC Scopus subject areas

  • Organic Chemistry

Citar esto

Salgado, F., Albornoz, L., Cortéz, C., Stashenko, E., Urrea-Vallejo, K., Nagles, E., ... Areche, C. (2018). Secondary metabolite profiling of species of the genus usnea by UHPLC-ESI-OT-MS-MS. Molecules, 23(1), [54]. https://doi.org/10.3390/molecules23010054
Salgado, Francisco ; Albornoz, Laura ; Cortéz, Carmen ; Stashenko, Elena ; Urrea-Vallejo, Kelly ; Nagles, Edgar ; Galicia-Virviescas, Cesar ; Cornejo, Alberto ; Ardiles, Alejandro ; Simirgiotis, Mario ; García-Beltrán, Olimpo ; Areche, Carlos. / Secondary metabolite profiling of species of the genus usnea by UHPLC-ESI-OT-MS-MS. En: Molecules. 2018 ; Vol. 23, N.º 1.
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abstract = "Lichens are symbiotic associations of fungi with microalgae and/or cyanobacteria, which are considered among the slowest growing organisms, with strong tolerance to adverse environmental conditions. There are about 400 genera and 1600 species of lichens and those belonging to the Usnea genus comprise about 360 of these species. Usnea lichens have been used since ancient times as dyes, cosmetics, preservatives, deodorants and folk medicines. The phytochemistry of the Usnea genus includes more than 60 compounds which belong to the following classes: depsides, depsidones, depsones, lactones, quinones, phenolics, polysaccharides, fatty acids and dibenzofurans. Due to scarce knowledge of metabolomic profiles of Usnea species (U. barbata, U. antarctica, U. rubicunda and U. subfloridana), a study based on UHPLC-ESI-OT-MS-MS was performed for a comprehensive characterization of their secondary metabolites. From the methanolic extracts of these species a total of 73 metabolites were identified for the first time using this hyphenated technique, including 34 compounds in U. barbata, 21 in U. antarctica, 38 in U. rubicunda and 37 in U. subfloridana. Besides, a total of 13 metabolites were not identified and reported so far, and could be new according to our data analysis. This study showed that this hyphenated technique is rapid, effective and accurate for phytochemical identification of lichen metabolites and the data collected could be useful for chemotaxonomic studies.",
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Salgado, F, Albornoz, L, Cortéz, C, Stashenko, E, Urrea-Vallejo, K, Nagles, E, Galicia-Virviescas, C, Cornejo, A, Ardiles, A, Simirgiotis, M, García-Beltrán, O & Areche, C 2018, 'Secondary metabolite profiling of species of the genus usnea by UHPLC-ESI-OT-MS-MS', Molecules, vol. 23, n.º 1, 54. https://doi.org/10.3390/molecules23010054

Secondary metabolite profiling of species of the genus usnea by UHPLC-ESI-OT-MS-MS. / Salgado, Francisco; Albornoz, Laura; Cortéz, Carmen; Stashenko, Elena; Urrea-Vallejo, Kelly; Nagles, Edgar; Galicia-Virviescas, Cesar; Cornejo, Alberto; Ardiles, Alejandro; Simirgiotis, Mario; García-Beltrán, Olimpo; Areche, Carlos.

En: Molecules, Vol. 23, N.º 1, 54, 01.01.2018.

Resultado de la investigación: Article

TY - JOUR

T1 - Secondary metabolite profiling of species of the genus usnea by UHPLC-ESI-OT-MS-MS

AU - Salgado, Francisco

AU - Albornoz, Laura

AU - Cortéz, Carmen

AU - Stashenko, Elena

AU - Urrea-Vallejo, Kelly

AU - Nagles, Edgar

AU - Galicia-Virviescas, Cesar

AU - Cornejo, Alberto

AU - Ardiles, Alejandro

AU - Simirgiotis, Mario

AU - García-Beltrán, Olimpo

AU - Areche, Carlos

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Lichens are symbiotic associations of fungi with microalgae and/or cyanobacteria, which are considered among the slowest growing organisms, with strong tolerance to adverse environmental conditions. There are about 400 genera and 1600 species of lichens and those belonging to the Usnea genus comprise about 360 of these species. Usnea lichens have been used since ancient times as dyes, cosmetics, preservatives, deodorants and folk medicines. The phytochemistry of the Usnea genus includes more than 60 compounds which belong to the following classes: depsides, depsidones, depsones, lactones, quinones, phenolics, polysaccharides, fatty acids and dibenzofurans. Due to scarce knowledge of metabolomic profiles of Usnea species (U. barbata, U. antarctica, U. rubicunda and U. subfloridana), a study based on UHPLC-ESI-OT-MS-MS was performed for a comprehensive characterization of their secondary metabolites. From the methanolic extracts of these species a total of 73 metabolites were identified for the first time using this hyphenated technique, including 34 compounds in U. barbata, 21 in U. antarctica, 38 in U. rubicunda and 37 in U. subfloridana. Besides, a total of 13 metabolites were not identified and reported so far, and could be new according to our data analysis. This study showed that this hyphenated technique is rapid, effective and accurate for phytochemical identification of lichen metabolites and the data collected could be useful for chemotaxonomic studies.

AB - Lichens are symbiotic associations of fungi with microalgae and/or cyanobacteria, which are considered among the slowest growing organisms, with strong tolerance to adverse environmental conditions. There are about 400 genera and 1600 species of lichens and those belonging to the Usnea genus comprise about 360 of these species. Usnea lichens have been used since ancient times as dyes, cosmetics, preservatives, deodorants and folk medicines. The phytochemistry of the Usnea genus includes more than 60 compounds which belong to the following classes: depsides, depsidones, depsones, lactones, quinones, phenolics, polysaccharides, fatty acids and dibenzofurans. Due to scarce knowledge of metabolomic profiles of Usnea species (U. barbata, U. antarctica, U. rubicunda and U. subfloridana), a study based on UHPLC-ESI-OT-MS-MS was performed for a comprehensive characterization of their secondary metabolites. From the methanolic extracts of these species a total of 73 metabolites were identified for the first time using this hyphenated technique, including 34 compounds in U. barbata, 21 in U. antarctica, 38 in U. rubicunda and 37 in U. subfloridana. Besides, a total of 13 metabolites were not identified and reported so far, and could be new according to our data analysis. This study showed that this hyphenated technique is rapid, effective and accurate for phytochemical identification of lichen metabolites and the data collected could be useful for chemotaxonomic studies.

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KW - Natural product

KW - Orbitrap

KW - UHPLC-MS-MS

KW - Usnea

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DO - 10.3390/molecules23010054

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Salgado F, Albornoz L, Cortéz C, Stashenko E, Urrea-Vallejo K, Nagles E y otros. Secondary metabolite profiling of species of the genus usnea by UHPLC-ESI-OT-MS-MS. Molecules. 2018 ene 1;23(1). 54. https://doi.org/10.3390/molecules23010054