Seasonal acclimatization of the carp involves differential expression of 5.8S ribosomal RNA in pituitary cells

María Inés Vera, Hernán M. Ríos, Erwin De La Fuente, Jaime Figueroa, Manuel Krauskopf

Resultado de la investigación: Article

24 Citas (Scopus)

Resumen

In the carp, nucleolar reorganization is one of the most remarkable features of the cellular adjustment that occurs upon seasonal acclimatization. To assess if the level of cellular expression of a specific rRNA is maintained between both seasonal adaptive states, we isolated a carp 5.8S rRNA gene. Sequence analyses showed its high conservation during evolution. The level of expression of 5.8S rRNA was determined by in situ hybridization assays in pituitary sections from summer- and winter- acclimatized carp. The quantification of the positive hybridization signals demonstrated a statistically significant difference between the acclimatization states. Transcription of 5.8S rRNA was considerably higher in summer- than in winter-acclimatized carp. These results indicate that the level of rRNA expression in eurythermal fish is not comparable between seasons and suggest that rRNA genes may be not constitutive. Thus, rRNA transcription should be used with caution as a reference to quantitate transcripts that are modulated as part of the acclimatization process in eurythermal fish.

Idioma originalEnglish
Páginas (desde-hasta)777-781
Número de páginas5
PublicaciónComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volumen118
N.º4
DOI
EstadoPublished - 1 dic 1997

Huella dactilar

5.8S Ribosomal RNA
Carps
Acclimatization
Transcription
Fish
Genes
rRNA Genes
Assays
Conservation
Fishes
In Situ Hybridization
Sequence Analysis

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

Citar esto

Vera, María Inés ; Ríos, Hernán M. ; De La Fuente, Erwin ; Figueroa, Jaime ; Krauskopf, Manuel. / Seasonal acclimatization of the carp involves differential expression of 5.8S ribosomal RNA in pituitary cells. En: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology. 1997 ; Vol. 118, N.º 4. pp. 777-781.
@article{cfe9ce694e884d7bab408f5556a6ae26,
title = "Seasonal acclimatization of the carp involves differential expression of 5.8S ribosomal RNA in pituitary cells",
abstract = "In the carp, nucleolar reorganization is one of the most remarkable features of the cellular adjustment that occurs upon seasonal acclimatization. To assess if the level of cellular expression of a specific rRNA is maintained between both seasonal adaptive states, we isolated a carp 5.8S rRNA gene. Sequence analyses showed its high conservation during evolution. The level of expression of 5.8S rRNA was determined by in situ hybridization assays in pituitary sections from summer- and winter- acclimatized carp. The quantification of the positive hybridization signals demonstrated a statistically significant difference between the acclimatization states. Transcription of 5.8S rRNA was considerably higher in summer- than in winter-acclimatized carp. These results indicate that the level of rRNA expression in eurythermal fish is not comparable between seasons and suggest that rRNA genes may be not constitutive. Thus, rRNA transcription should be used with caution as a reference to quantitate transcripts that are modulated as part of the acclimatization process in eurythermal fish.",
keywords = "5.8S rRNA, Acclimatization, Carp, Nucleolus, rRNA expression",
author = "Vera, {Mar{\'i}a In{\'e}s} and R{\'i}os, {Hern{\'a}n M.} and {De La Fuente}, Erwin and Jaime Figueroa and Manuel Krauskopf",
year = "1997",
month = "12",
day = "1",
doi = "10.1016/S0305-0491(97)00271-X",
language = "English",
volume = "118",
pages = "777--781",
journal = "Comparative biochemistry and physiology. B, Comparative biochemistry",
issn = "1096-4959",
publisher = "Elsevier Inc.",
number = "4",

}

Seasonal acclimatization of the carp involves differential expression of 5.8S ribosomal RNA in pituitary cells. / Vera, María Inés; Ríos, Hernán M.; De La Fuente, Erwin; Figueroa, Jaime; Krauskopf, Manuel.

En: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, Vol. 118, N.º 4, 01.12.1997, p. 777-781.

Resultado de la investigación: Article

TY - JOUR

T1 - Seasonal acclimatization of the carp involves differential expression of 5.8S ribosomal RNA in pituitary cells

AU - Vera, María Inés

AU - Ríos, Hernán M.

AU - De La Fuente, Erwin

AU - Figueroa, Jaime

AU - Krauskopf, Manuel

PY - 1997/12/1

Y1 - 1997/12/1

N2 - In the carp, nucleolar reorganization is one of the most remarkable features of the cellular adjustment that occurs upon seasonal acclimatization. To assess if the level of cellular expression of a specific rRNA is maintained between both seasonal adaptive states, we isolated a carp 5.8S rRNA gene. Sequence analyses showed its high conservation during evolution. The level of expression of 5.8S rRNA was determined by in situ hybridization assays in pituitary sections from summer- and winter- acclimatized carp. The quantification of the positive hybridization signals demonstrated a statistically significant difference between the acclimatization states. Transcription of 5.8S rRNA was considerably higher in summer- than in winter-acclimatized carp. These results indicate that the level of rRNA expression in eurythermal fish is not comparable between seasons and suggest that rRNA genes may be not constitutive. Thus, rRNA transcription should be used with caution as a reference to quantitate transcripts that are modulated as part of the acclimatization process in eurythermal fish.

AB - In the carp, nucleolar reorganization is one of the most remarkable features of the cellular adjustment that occurs upon seasonal acclimatization. To assess if the level of cellular expression of a specific rRNA is maintained between both seasonal adaptive states, we isolated a carp 5.8S rRNA gene. Sequence analyses showed its high conservation during evolution. The level of expression of 5.8S rRNA was determined by in situ hybridization assays in pituitary sections from summer- and winter- acclimatized carp. The quantification of the positive hybridization signals demonstrated a statistically significant difference between the acclimatization states. Transcription of 5.8S rRNA was considerably higher in summer- than in winter-acclimatized carp. These results indicate that the level of rRNA expression in eurythermal fish is not comparable between seasons and suggest that rRNA genes may be not constitutive. Thus, rRNA transcription should be used with caution as a reference to quantitate transcripts that are modulated as part of the acclimatization process in eurythermal fish.

KW - 5.8S rRNA

KW - Acclimatization

KW - Carp

KW - Nucleolus

KW - rRNA expression

UR - http://www.scopus.com/inward/record.url?scp=0031407303&partnerID=8YFLogxK

U2 - 10.1016/S0305-0491(97)00271-X

DO - 10.1016/S0305-0491(97)00271-X

M3 - Article

AN - SCOPUS:0031407303

VL - 118

SP - 777

EP - 781

JO - Comparative biochemistry and physiology. B, Comparative biochemistry

JF - Comparative biochemistry and physiology. B, Comparative biochemistry

SN - 1096-4959

IS - 4

ER -