Salmonella Typhi shdA: Pseudogene or allelic variant?

I. M. Urrutia, J. A. Fuentes, L. M. Valenzuela, A. P. Ortega, A. A. Hidalgo, G. C. Mora

Resultado de la investigación: Article

9 Citas (Scopus)

Resumen

ShdA from Salmonella Typhimurium (ShdASTm) is a large outer membrane protein that specifically recognizes and binds to fibronectin. ShdASTm is involved in the colonization of the cecum and the Peyer's patches of terminal ileum in mice. On the other hand, shdA gene from Salmonella Typhi (shdASTy) has been considered a pseudogene (i.e. a nonfunctional sequence of genomic DNA) due to the presence of deletions and mutations that gave rise to premature stop codons. In this work we show that, despite the deletions and mutations, shdASTy is fully functional. S. Typhi δshdA mutants presented an impaired adherence and invasion of HEp-2 pre-treated with TGF-β1, an inducer of fibronectin production. Moreover, shdA from S. Typhi and S. Typhimurium seem to be equivalent since shdASTm restored the adherence and invasion of S. Typhi δshdA mutant to wild type levels. In addition, anti-FLAG mAbs interfered with the adherence and invasion of the S. Typhi shdA-3xFLAG strain. Finally, shdASTy encodes a detectable protein when heterologously expressed in Escherichia coli DH5α.The data presented here show that shdASTy is not a pseudogene, but a different functional allele compared with shdASTm.

Idioma originalEnglish
Páginas (desde-hasta)146-152
Número de páginas7
PublicaciónInfection, Genetics and Evolution
Volumen26
DOI
EstadoPublished - 1 ene 2014

Huella dactilar

Salmonella Typhi
Salmonella typhi
Pseudogenes
pseudogenes
gene
fibronectins
Sequence Deletion
Salmonella typhimurium
mutation
Fibronectins
Salmonella Typhimurium
Genes
genes
protein
Peyer's Patches
mutants
Peyer's patches
Cecum
outer membrane proteins
stop codon

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Microbiology (medical)
  • Infectious Diseases

Citar esto

Urrutia, I. M. ; Fuentes, J. A. ; Valenzuela, L. M. ; Ortega, A. P. ; Hidalgo, A. A. ; Mora, G. C. / Salmonella Typhi shdA : Pseudogene or allelic variant?. En: Infection, Genetics and Evolution. 2014 ; Vol. 26. pp. 146-152.
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title = "Salmonella Typhi shdA: Pseudogene or allelic variant?",
abstract = "ShdA from Salmonella Typhimurium (ShdASTm) is a large outer membrane protein that specifically recognizes and binds to fibronectin. ShdASTm is involved in the colonization of the cecum and the Peyer's patches of terminal ileum in mice. On the other hand, shdA gene from Salmonella Typhi (shdASTy) has been considered a pseudogene (i.e. a nonfunctional sequence of genomic DNA) due to the presence of deletions and mutations that gave rise to premature stop codons. In this work we show that, despite the deletions and mutations, shdASTy is fully functional. S. Typhi δshdA mutants presented an impaired adherence and invasion of HEp-2 pre-treated with TGF-β1, an inducer of fibronectin production. Moreover, shdA from S. Typhi and S. Typhimurium seem to be equivalent since shdASTm restored the adherence and invasion of S. Typhi δshdA mutant to wild type levels. In addition, anti-FLAG mAbs interfered with the adherence and invasion of the S. Typhi shdA-3xFLAG strain. Finally, shdASTy encodes a detectable protein when heterologously expressed in Escherichia coli DH5α.The data presented here show that shdASTy is not a pseudogene, but a different functional allele compared with shdASTm.",
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Salmonella Typhi shdA : Pseudogene or allelic variant? / Urrutia, I. M.; Fuentes, J. A.; Valenzuela, L. M.; Ortega, A. P.; Hidalgo, A. A.; Mora, G. C.

En: Infection, Genetics and Evolution, Vol. 26, 01.01.2014, p. 146-152.

Resultado de la investigación: Article

TY - JOUR

T1 - Salmonella Typhi shdA

T2 - Pseudogene or allelic variant?

AU - Urrutia, I. M.

AU - Fuentes, J. A.

AU - Valenzuela, L. M.

AU - Ortega, A. P.

AU - Hidalgo, A. A.

AU - Mora, G. C.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - ShdA from Salmonella Typhimurium (ShdASTm) is a large outer membrane protein that specifically recognizes and binds to fibronectin. ShdASTm is involved in the colonization of the cecum and the Peyer's patches of terminal ileum in mice. On the other hand, shdA gene from Salmonella Typhi (shdASTy) has been considered a pseudogene (i.e. a nonfunctional sequence of genomic DNA) due to the presence of deletions and mutations that gave rise to premature stop codons. In this work we show that, despite the deletions and mutations, shdASTy is fully functional. S. Typhi δshdA mutants presented an impaired adherence and invasion of HEp-2 pre-treated with TGF-β1, an inducer of fibronectin production. Moreover, shdA from S. Typhi and S. Typhimurium seem to be equivalent since shdASTm restored the adherence and invasion of S. Typhi δshdA mutant to wild type levels. In addition, anti-FLAG mAbs interfered with the adherence and invasion of the S. Typhi shdA-3xFLAG strain. Finally, shdASTy encodes a detectable protein when heterologously expressed in Escherichia coli DH5α.The data presented here show that shdASTy is not a pseudogene, but a different functional allele compared with shdASTm.

AB - ShdA from Salmonella Typhimurium (ShdASTm) is a large outer membrane protein that specifically recognizes and binds to fibronectin. ShdASTm is involved in the colonization of the cecum and the Peyer's patches of terminal ileum in mice. On the other hand, shdA gene from Salmonella Typhi (shdASTy) has been considered a pseudogene (i.e. a nonfunctional sequence of genomic DNA) due to the presence of deletions and mutations that gave rise to premature stop codons. In this work we show that, despite the deletions and mutations, shdASTy is fully functional. S. Typhi δshdA mutants presented an impaired adherence and invasion of HEp-2 pre-treated with TGF-β1, an inducer of fibronectin production. Moreover, shdA from S. Typhi and S. Typhimurium seem to be equivalent since shdASTm restored the adherence and invasion of S. Typhi δshdA mutant to wild type levels. In addition, anti-FLAG mAbs interfered with the adherence and invasion of the S. Typhi shdA-3xFLAG strain. Finally, shdASTy encodes a detectable protein when heterologously expressed in Escherichia coli DH5α.The data presented here show that shdASTy is not a pseudogene, but a different functional allele compared with shdASTm.

KW - Fibronectin

KW - Host-specificity

KW - Pseudogene

KW - ShdA

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JO - Infection, Genetics and Evolution

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