Salmonella typhi mutants defective in anaerobic respiration are impaired in their ability to replicate within epithelial cells

Inès Contreras, Cecilia S. Toro, Gonzalo Troncoso, Guido C. Mora

Resultado de la investigación: Article

41 Citas (Scopus)

Resumen

By using MudJ (Kan, lac)-directed operon fusion technology, mutants of Salmonella typhi whose gene expression is induced under anaerobic growth conditions were isolated. Characterization of their phenotypes and regulatory properties revealed that two of the mutants were unable to use nitrate as a terminal electron acceptor in the absence of oxygen, suggesting that they were defective in nitrate reductase activity. Anaerobic induction of these fusions did not further increase in response to nitrate. Strains carrying an additional mutation in oxrA were constructed.They showed a lower level of β-galactosidase expression both aerobically and anaerobically; however, the ratios of anaerobic induction remained unaltered. These MudJ insertions mapped to the 17-19 min region of the chromosome. Based upon their phenotypes and mapping, one of the mutants probably possessed a modC (chlD)::MudJ insertion and the other a moaA (chlA)::MudJ insertion. A third mutant was unable to use either nitrate or fumarate as a terminal electron acceptor. All three mutants showed a reduced ability to enter into and proliferate within HEp-2 epithelial cells. The oxrA mutation enhanced entry and proliferation of both the wild-type cells and the three mutants. Taken together, these results suggest that anaerobic respiration plays a role in S. typhi invasiveness.

Idioma originalEnglish
Páginas (desde-hasta)2665-2672
Número de páginas8
PublicaciónMicrobiology
Volumen143
N.º8
EstadoPublished - ago 1997

Huella dactilar

Salmonella typhi
Nitrates
Respiration
Epithelial Cells
Galactosidases
Electrons
Phenotype
Nitrate Reductase
Fumarates
Mutation
Lac Operon
Chromosomes
Oxygen
Technology
Gene Expression
Growth

ASJC Scopus subject areas

  • Microbiology

Citar esto

Contreras, Inès ; Toro, Cecilia S. ; Troncoso, Gonzalo ; Mora, Guido C. / Salmonella typhi mutants defective in anaerobic respiration are impaired in their ability to replicate within epithelial cells. En: Microbiology. 1997 ; Vol. 143, N.º 8. pp. 2665-2672.
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abstract = "By using MudJ (Kan, lac)-directed operon fusion technology, mutants of Salmonella typhi whose gene expression is induced under anaerobic growth conditions were isolated. Characterization of their phenotypes and regulatory properties revealed that two of the mutants were unable to use nitrate as a terminal electron acceptor in the absence of oxygen, suggesting that they were defective in nitrate reductase activity. Anaerobic induction of these fusions did not further increase in response to nitrate. Strains carrying an additional mutation in oxrA were constructed.They showed a lower level of β-galactosidase expression both aerobically and anaerobically; however, the ratios of anaerobic induction remained unaltered. These MudJ insertions mapped to the 17-19 min region of the chromosome. Based upon their phenotypes and mapping, one of the mutants probably possessed a modC (chlD)::MudJ insertion and the other a moaA (chlA)::MudJ insertion. A third mutant was unable to use either nitrate or fumarate as a terminal electron acceptor. All three mutants showed a reduced ability to enter into and proliferate within HEp-2 epithelial cells. The oxrA mutation enhanced entry and proliferation of both the wild-type cells and the three mutants. Taken together, these results suggest that anaerobic respiration plays a role in S. typhi invasiveness.",
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Salmonella typhi mutants defective in anaerobic respiration are impaired in their ability to replicate within epithelial cells. / Contreras, Inès; Toro, Cecilia S.; Troncoso, Gonzalo; Mora, Guido C.

En: Microbiology, Vol. 143, N.º 8, 08.1997, p. 2665-2672.

Resultado de la investigación: Article

TY - JOUR

T1 - Salmonella typhi mutants defective in anaerobic respiration are impaired in their ability to replicate within epithelial cells

AU - Contreras, Inès

AU - Toro, Cecilia S.

AU - Troncoso, Gonzalo

AU - Mora, Guido C.

PY - 1997/8

Y1 - 1997/8

N2 - By using MudJ (Kan, lac)-directed operon fusion technology, mutants of Salmonella typhi whose gene expression is induced under anaerobic growth conditions were isolated. Characterization of their phenotypes and regulatory properties revealed that two of the mutants were unable to use nitrate as a terminal electron acceptor in the absence of oxygen, suggesting that they were defective in nitrate reductase activity. Anaerobic induction of these fusions did not further increase in response to nitrate. Strains carrying an additional mutation in oxrA were constructed.They showed a lower level of β-galactosidase expression both aerobically and anaerobically; however, the ratios of anaerobic induction remained unaltered. These MudJ insertions mapped to the 17-19 min region of the chromosome. Based upon their phenotypes and mapping, one of the mutants probably possessed a modC (chlD)::MudJ insertion and the other a moaA (chlA)::MudJ insertion. A third mutant was unable to use either nitrate or fumarate as a terminal electron acceptor. All three mutants showed a reduced ability to enter into and proliferate within HEp-2 epithelial cells. The oxrA mutation enhanced entry and proliferation of both the wild-type cells and the three mutants. Taken together, these results suggest that anaerobic respiration plays a role in S. typhi invasiveness.

AB - By using MudJ (Kan, lac)-directed operon fusion technology, mutants of Salmonella typhi whose gene expression is induced under anaerobic growth conditions were isolated. Characterization of their phenotypes and regulatory properties revealed that two of the mutants were unable to use nitrate as a terminal electron acceptor in the absence of oxygen, suggesting that they were defective in nitrate reductase activity. Anaerobic induction of these fusions did not further increase in response to nitrate. Strains carrying an additional mutation in oxrA were constructed.They showed a lower level of β-galactosidase expression both aerobically and anaerobically; however, the ratios of anaerobic induction remained unaltered. These MudJ insertions mapped to the 17-19 min region of the chromosome. Based upon their phenotypes and mapping, one of the mutants probably possessed a modC (chlD)::MudJ insertion and the other a moaA (chlA)::MudJ insertion. A third mutant was unable to use either nitrate or fumarate as a terminal electron acceptor. All three mutants showed a reduced ability to enter into and proliferate within HEp-2 epithelial cells. The oxrA mutation enhanced entry and proliferation of both the wild-type cells and the three mutants. Taken together, these results suggest that anaerobic respiration plays a role in S. typhi invasiveness.

KW - Anaerobiosis

KW - Invasiveness

KW - Nitrate respiration

KW - Salmonella typhi

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