Salmonella enterica serovar Typhimurium BaeSR two-component system positively regulates sodA in response to ciprofloxacin

P. Guerrero, B. Collao, R. Álvarez, H. Salinas, E. H. Morales, I. L. Calderón, C. P. Saavedra, F. Gil

Resultado de la investigación: Article

11 Citas (Scopus)

Resumen

In response to antibiotics, bacteria activate regulatory systems that control the expression of genes that participate in detoxifying these compounds, like multidrug efflux systems. We previously demonstrated that the BaeSR two-component system from Salmonella enterica serovar Typhimurium (S. Typhimurium) participates in the detection of ciprofloxacin, a bactericidal antibiotic, and in the positive regulation of mdtA, an efflux pump implicated in antibiotic resistance. In the present work, we provide further evidence for a role of the S. Typhimurium BaeSR two-component system in response to ciprofloxacin treatment and show that it regulates sodA expression. We demonstrate that, in the absence of BaeSR, the transcript levels of sodA and the activity of its gene product are lower. Using electrophoretic mobility shift assays and transcriptional fusions, we demonstrate that BaeR regulates sodA by a direct interaction with the promoter region.

Idioma originalEnglish
Páginas (desde-hasta)2049-2057
Número de páginas9
PublicaciónMicrobiology (United Kingdom)
Volumen159
N.ºPART 10
DOI
EstadoPublished - oct 2013

Huella dactilar

Salmonella enterica
Ciprofloxacin
Anti-Bacterial Agents
Electrophoretic Mobility Shift Assay
Microbial Drug Resistance
Genetic Promoter Regions
Bacteria
Gene Expression
Genes
Serogroup

ASJC Scopus subject areas

  • Microbiology

Citar esto

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title = "Salmonella enterica serovar Typhimurium BaeSR two-component system positively regulates sodA in response to ciprofloxacin",
abstract = "In response to antibiotics, bacteria activate regulatory systems that control the expression of genes that participate in detoxifying these compounds, like multidrug efflux systems. We previously demonstrated that the BaeSR two-component system from Salmonella enterica serovar Typhimurium (S. Typhimurium) participates in the detection of ciprofloxacin, a bactericidal antibiotic, and in the positive regulation of mdtA, an efflux pump implicated in antibiotic resistance. In the present work, we provide further evidence for a role of the S. Typhimurium BaeSR two-component system in response to ciprofloxacin treatment and show that it regulates sodA expression. We demonstrate that, in the absence of BaeSR, the transcript levels of sodA and the activity of its gene product are lower. Using electrophoretic mobility shift assays and transcriptional fusions, we demonstrate that BaeR regulates sodA by a direct interaction with the promoter region.",
author = "P. Guerrero and B. Collao and R. {\'A}lvarez and H. Salinas and Morales, {E. H.} and Calder{\'o}n, {I. L.} and Saavedra, {C. P.} and F. Gil",
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Salmonella enterica serovar Typhimurium BaeSR two-component system positively regulates sodA in response to ciprofloxacin. / Guerrero, P.; Collao, B.; Álvarez, R.; Salinas, H.; Morales, E. H.; Calderón, I. L.; Saavedra, C. P.; Gil, F.

En: Microbiology (United Kingdom), Vol. 159, N.º PART 10, 10.2013, p. 2049-2057.

Resultado de la investigación: Article

TY - JOUR

T1 - Salmonella enterica serovar Typhimurium BaeSR two-component system positively regulates sodA in response to ciprofloxacin

AU - Guerrero, P.

AU - Collao, B.

AU - Álvarez, R.

AU - Salinas, H.

AU - Morales, E. H.

AU - Calderón, I. L.

AU - Saavedra, C. P.

AU - Gil, F.

PY - 2013/10

Y1 - 2013/10

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AB - In response to antibiotics, bacteria activate regulatory systems that control the expression of genes that participate in detoxifying these compounds, like multidrug efflux systems. We previously demonstrated that the BaeSR two-component system from Salmonella enterica serovar Typhimurium (S. Typhimurium) participates in the detection of ciprofloxacin, a bactericidal antibiotic, and in the positive regulation of mdtA, an efflux pump implicated in antibiotic resistance. In the present work, we provide further evidence for a role of the S. Typhimurium BaeSR two-component system in response to ciprofloxacin treatment and show that it regulates sodA expression. We demonstrate that, in the absence of BaeSR, the transcript levels of sodA and the activity of its gene product are lower. Using electrophoretic mobility shift assays and transcriptional fusions, we demonstrate that BaeR regulates sodA by a direct interaction with the promoter region.

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