Salmonella enterica serovar Typhimurium BaeSR two-component system positively regulates sodA in response to ciprofloxacin

P. Guerrero, B. Collao, R. Álvarez, H. Salinas, E. H. Morales, I. L. Calderón, C. P. Saavedra, F. Gil

Resultado de la investigación: Contribución a una revistaArtículo

11 Citas (Scopus)

Resumen

In response to antibiotics, bacteria activate regulatory systems that control the expression of genes that participate in detoxifying these compounds, like multidrug efflux systems. We previously demonstrated that the BaeSR two-component system from Salmonella enterica serovar Typhimurium (S. Typhimurium) participates in the detection of ciprofloxacin, a bactericidal antibiotic, and in the positive regulation of mdtA, an efflux pump implicated in antibiotic resistance. In the present work, we provide further evidence for a role of the S. Typhimurium BaeSR two-component system in response to ciprofloxacin treatment and show that it regulates sodA expression. We demonstrate that, in the absence of BaeSR, the transcript levels of sodA and the activity of its gene product are lower. Using electrophoretic mobility shift assays and transcriptional fusions, we demonstrate that BaeR regulates sodA by a direct interaction with the promoter region.

Idioma originalInglés
Páginas (desde-hasta)2049-2057
Número de páginas9
PublicaciónMicrobiology (United Kingdom)
Volumen159
N.ºPART 10
DOI
EstadoPublicada - oct 2013

Áreas temáticas de ASJC Scopus

  • Microbiología

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