Salmonella bacteriophage diversity reflects host diversity on dairy farms

Andrea I. Moreno Switt, Henk C. den Bakker, Kitiya Vongkamjan, Karin Hoelzer, Lorin D. Warnick, Kevin J. Cummings, Martin Wiedmann

Resultado de la investigación: Article

22 Citas (Scopus)

Resumen

Salmonella is an animal and human pathogen of worldwide concern. Surveillance programs indicate that the incidence of Salmonella serovars fluctuates over time. While bacteriophages are likely to play a role in driving microbial diversity, our understanding of the ecology and diversity of Salmonella phages is limited. Here we report the isolation of Salmonella phages from manure samples from 13 dairy farms with a history of Salmonella presence. Salmonella phages were isolated from 10 of the 13 farms; overall 108 phage isolates were obtained on serovar Newport, Typhimurium, Dublin, Kentucky, Anatum, Mbandaka, and Cerro hosts. Host range characterization found that 51% of phage isolates had a narrow host range, while 49% showed a broad host range. The phage isolates represented 65 lysis profiles; genome size profiling of 94 phage isolates allowed for classification of phage isolates into 11 groups with subsequent restriction fragment length polymorphism analysis showing considerable variation within a given group. Our data not only show an abundance of diverse Salmonella phage isolates in dairy farms, but also show that phage isolates that lyse the most common serovars causing salmonellosis in cattle are frequently obtained, suggesting that phages may play an important role in the ecology of Salmonella on dairy farms.

Idioma originalEnglish
Páginas (desde-hasta)275-285
Número de páginas11
PublicaciónFood Microbiology
Volumen36
N.º2
DOI
EstadoPublished - 1 dic 2013

Huella dactilar

Salmonella Phages
dairy farming
bacteriophages
Bacteriophages
Salmonella
Host Specificity
Ecology
host range
serotypes
Genome Size
Manure
Farms
Salmonella Infections
Restriction Fragment Length Polymorphisms
ecology
salmonellosis
animal manures

ASJC Scopus subject areas

  • Food Science
  • Microbiology

Citar esto

Moreno Switt, A. I., den Bakker, H. C., Vongkamjan, K., Hoelzer, K., Warnick, L. D., Cummings, K. J., & Wiedmann, M. (2013). Salmonella bacteriophage diversity reflects host diversity on dairy farms. Food Microbiology, 36(2), 275-285. https://doi.org/10.1016/j.fm.2013.06.014
Moreno Switt, Andrea I. ; den Bakker, Henk C. ; Vongkamjan, Kitiya ; Hoelzer, Karin ; Warnick, Lorin D. ; Cummings, Kevin J. ; Wiedmann, Martin. / Salmonella bacteriophage diversity reflects host diversity on dairy farms. En: Food Microbiology. 2013 ; Vol. 36, N.º 2. pp. 275-285.
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Moreno Switt, AI, den Bakker, HC, Vongkamjan, K, Hoelzer, K, Warnick, LD, Cummings, KJ & Wiedmann, M 2013, 'Salmonella bacteriophage diversity reflects host diversity on dairy farms', Food Microbiology, vol. 36, n.º 2, pp. 275-285. https://doi.org/10.1016/j.fm.2013.06.014

Salmonella bacteriophage diversity reflects host diversity on dairy farms. / Moreno Switt, Andrea I.; den Bakker, Henk C.; Vongkamjan, Kitiya; Hoelzer, Karin; Warnick, Lorin D.; Cummings, Kevin J.; Wiedmann, Martin.

En: Food Microbiology, Vol. 36, N.º 2, 01.12.2013, p. 275-285.

Resultado de la investigación: Article

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AU - den Bakker, Henk C.

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AU - Hoelzer, Karin

AU - Warnick, Lorin D.

AU - Cummings, Kevin J.

AU - Wiedmann, Martin

PY - 2013/12/1

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N2 - Salmonella is an animal and human pathogen of worldwide concern. Surveillance programs indicate that the incidence of Salmonella serovars fluctuates over time. While bacteriophages are likely to play a role in driving microbial diversity, our understanding of the ecology and diversity of Salmonella phages is limited. Here we report the isolation of Salmonella phages from manure samples from 13 dairy farms with a history of Salmonella presence. Salmonella phages were isolated from 10 of the 13 farms; overall 108 phage isolates were obtained on serovar Newport, Typhimurium, Dublin, Kentucky, Anatum, Mbandaka, and Cerro hosts. Host range characterization found that 51% of phage isolates had a narrow host range, while 49% showed a broad host range. The phage isolates represented 65 lysis profiles; genome size profiling of 94 phage isolates allowed for classification of phage isolates into 11 groups with subsequent restriction fragment length polymorphism analysis showing considerable variation within a given group. Our data not only show an abundance of diverse Salmonella phage isolates in dairy farms, but also show that phage isolates that lyse the most common serovars causing salmonellosis in cattle are frequently obtained, suggesting that phages may play an important role in the ecology of Salmonella on dairy farms.

AB - Salmonella is an animal and human pathogen of worldwide concern. Surveillance programs indicate that the incidence of Salmonella serovars fluctuates over time. While bacteriophages are likely to play a role in driving microbial diversity, our understanding of the ecology and diversity of Salmonella phages is limited. Here we report the isolation of Salmonella phages from manure samples from 13 dairy farms with a history of Salmonella presence. Salmonella phages were isolated from 10 of the 13 farms; overall 108 phage isolates were obtained on serovar Newport, Typhimurium, Dublin, Kentucky, Anatum, Mbandaka, and Cerro hosts. Host range characterization found that 51% of phage isolates had a narrow host range, while 49% showed a broad host range. The phage isolates represented 65 lysis profiles; genome size profiling of 94 phage isolates allowed for classification of phage isolates into 11 groups with subsequent restriction fragment length polymorphism analysis showing considerable variation within a given group. Our data not only show an abundance of diverse Salmonella phage isolates in dairy farms, but also show that phage isolates that lyse the most common serovars causing salmonellosis in cattle are frequently obtained, suggesting that phages may play an important role in the ecology of Salmonella on dairy farms.

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Moreno Switt AI, den Bakker HC, Vongkamjan K, Hoelzer K, Warnick LD, Cummings KJ y otros. Salmonella bacteriophage diversity reflects host diversity on dairy farms. Food Microbiology. 2013 dic 1;36(2):275-285. https://doi.org/10.1016/j.fm.2013.06.014