RpoS integrates CRP, Fis, and PhoP signaling pathways to control Salmonella typhi hlyE expression

Matí R. Jofré, Leonardo Rodríguez, Nicolás A. Villagra, Alejandro A. Hidalgo, Guido C. Mora, Juan A. Fuentes

Resultado de la investigación: Article

7 Citas (Scopus)

Resumen

Background: SPI-18 is a pathogenicity island found in some Salmonella enterica serovars, including S. Typhi. SPI-18 harbors two ORFs organized into an operon, hlyE and taiA genes, both implicated in virulence. Regarding the hlyE regulation in S. Typhi, it has been reported that RpoS participates as transcriptional up-regulator under low pH and high osmolarity. In addition, CRP down-regulates hlyE expression during exponential growth. Previously, it has been suggested that there is another factor related to catabolite repression, different from CRP, involved in the down-regulation of hlyE. Moreover, PhoP-dependent hlyE up-regulation has been reported in bacteria cultured simultaneously under low pH and low concentration of Mg2+. Nevertheless, the relative contribution of each environmental signal is not completely clear. In this work we aimed to better understand the regulation of hlyE in S. Typhi and the integration of different environmental signals through global regulators. Results: We found that Fis participates as a CRP-independent glucose-dependent down-regulator of hlyE. Also, Fis and CRP seem to exert the repression over hlyE through down-regulating rpoS. Moreover, PhoP up-regulates hlyE expression via rpoS under low pH and low Mg2+ conditions. Conclusions: All these results together show that, at least under the tested conditions, RpoS is the central regulator in the hlyE regulatory network, integrating multiple environmental signals and global regulators.

Idioma originalEnglish
PublicaciónBMC Microbiology
Volumen14
N.º1
DOI
EstadoPublished - 31 may 2014

Huella dactilar

Salmonella typhi
Up-Regulation
Down-Regulation
Catabolite Repression
Genomic Islands
Salmonella enterica
Operon
Osmolar Concentration
Open Reading Frames
Virulence
Bacteria
Glucose
Growth
Genes

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Citar esto

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title = "RpoS integrates CRP, Fis, and PhoP signaling pathways to control Salmonella typhi hlyE expression",
abstract = "Background: SPI-18 is a pathogenicity island found in some Salmonella enterica serovars, including S. Typhi. SPI-18 harbors two ORFs organized into an operon, hlyE and taiA genes, both implicated in virulence. Regarding the hlyE regulation in S. Typhi, it has been reported that RpoS participates as transcriptional up-regulator under low pH and high osmolarity. In addition, CRP down-regulates hlyE expression during exponential growth. Previously, it has been suggested that there is another factor related to catabolite repression, different from CRP, involved in the down-regulation of hlyE. Moreover, PhoP-dependent hlyE up-regulation has been reported in bacteria cultured simultaneously under low pH and low concentration of Mg2+. Nevertheless, the relative contribution of each environmental signal is not completely clear. In this work we aimed to better understand the regulation of hlyE in S. Typhi and the integration of different environmental signals through global regulators. Results: We found that Fis participates as a CRP-independent glucose-dependent down-regulator of hlyE. Also, Fis and CRP seem to exert the repression over hlyE through down-regulating rpoS. Moreover, PhoP up-regulates hlyE expression via rpoS under low pH and low Mg2+ conditions. Conclusions: All these results together show that, at least under the tested conditions, RpoS is the central regulator in the hlyE regulatory network, integrating multiple environmental signals and global regulators.",
keywords = "CAMP-receptor protein, Catabolite repression, Fis protein, HlyE, PhoP, RpoS",
author = "Jofr{\'e}, {Mat{\'i} R.} and Leonardo Rodr{\'i}guez and Villagra, {Nicol{\'a}s A.} and Hidalgo, {Alejandro A.} and Mora, {Guido C.} and Fuentes, {Juan A.}",
year = "2014",
month = "5",
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language = "English",
volume = "14",
journal = "BMC Microbiology",
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RpoS integrates CRP, Fis, and PhoP signaling pathways to control Salmonella typhi hlyE expression. / Jofré, Matí R.; Rodríguez, Leonardo; Villagra, Nicolás A.; Hidalgo, Alejandro A.; Mora, Guido C.; Fuentes, Juan A.

En: BMC Microbiology, Vol. 14, N.º 1, 31.05.2014.

Resultado de la investigación: Article

TY - JOUR

T1 - RpoS integrates CRP, Fis, and PhoP signaling pathways to control Salmonella typhi hlyE expression

AU - Jofré, Matí R.

AU - Rodríguez, Leonardo

AU - Villagra, Nicolás A.

AU - Hidalgo, Alejandro A.

AU - Mora, Guido C.

AU - Fuentes, Juan A.

PY - 2014/5/31

Y1 - 2014/5/31

N2 - Background: SPI-18 is a pathogenicity island found in some Salmonella enterica serovars, including S. Typhi. SPI-18 harbors two ORFs organized into an operon, hlyE and taiA genes, both implicated in virulence. Regarding the hlyE regulation in S. Typhi, it has been reported that RpoS participates as transcriptional up-regulator under low pH and high osmolarity. In addition, CRP down-regulates hlyE expression during exponential growth. Previously, it has been suggested that there is another factor related to catabolite repression, different from CRP, involved in the down-regulation of hlyE. Moreover, PhoP-dependent hlyE up-regulation has been reported in bacteria cultured simultaneously under low pH and low concentration of Mg2+. Nevertheless, the relative contribution of each environmental signal is not completely clear. In this work we aimed to better understand the regulation of hlyE in S. Typhi and the integration of different environmental signals through global regulators. Results: We found that Fis participates as a CRP-independent glucose-dependent down-regulator of hlyE. Also, Fis and CRP seem to exert the repression over hlyE through down-regulating rpoS. Moreover, PhoP up-regulates hlyE expression via rpoS under low pH and low Mg2+ conditions. Conclusions: All these results together show that, at least under the tested conditions, RpoS is the central regulator in the hlyE regulatory network, integrating multiple environmental signals and global regulators.

AB - Background: SPI-18 is a pathogenicity island found in some Salmonella enterica serovars, including S. Typhi. SPI-18 harbors two ORFs organized into an operon, hlyE and taiA genes, both implicated in virulence. Regarding the hlyE regulation in S. Typhi, it has been reported that RpoS participates as transcriptional up-regulator under low pH and high osmolarity. In addition, CRP down-regulates hlyE expression during exponential growth. Previously, it has been suggested that there is another factor related to catabolite repression, different from CRP, involved in the down-regulation of hlyE. Moreover, PhoP-dependent hlyE up-regulation has been reported in bacteria cultured simultaneously under low pH and low concentration of Mg2+. Nevertheless, the relative contribution of each environmental signal is not completely clear. In this work we aimed to better understand the regulation of hlyE in S. Typhi and the integration of different environmental signals through global regulators. Results: We found that Fis participates as a CRP-independent glucose-dependent down-regulator of hlyE. Also, Fis and CRP seem to exert the repression over hlyE through down-regulating rpoS. Moreover, PhoP up-regulates hlyE expression via rpoS under low pH and low Mg2+ conditions. Conclusions: All these results together show that, at least under the tested conditions, RpoS is the central regulator in the hlyE regulatory network, integrating multiple environmental signals and global regulators.

KW - CAMP-receptor protein

KW - Catabolite repression

KW - Fis protein

KW - HlyE

KW - PhoP

KW - RpoS

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U2 - 10.1186/1471-2180-14-139

DO - 10.1186/1471-2180-14-139

M3 - Article

C2 - 24885225

AN - SCOPUS:84923858846

VL - 14

JO - BMC Microbiology

JF - BMC Microbiology

SN - 1471-2180

IS - 1

ER -