Roles of DacB and Spm proteins in Clostridium perfringens spore resistance to moist heat, chemicals, and UV radiation

Daniel Paredes-Sabja, Nahid Sarker, Barbara Setlow, Peter Setlow, Mahfuzur R. Sarker

Resultado de la investigación: Article

39 Citas (Scopus)

Resumen

Clostridium perfringens food poisoning is caused mainly by enterotoxigenic type A isolates that typically possess high spore heat resistance. Previous studies have shown that α/β-type small, acid-soluble proteins (SASP) play a major role in the resistance of Bacillus subtilis and C. perfringens spores to moist heat, UV radiation, and some chemicals. Additional major factors in B. subtilis spore resistance are the spore's core water content and cortex peptidoglycan (PG) structure, with the latter properties modulated by the spm and dacB gene products and the sporulation temperature. In the current work, we have shown that the spm and dacB genes are expressed only during C. perfringens sporulation and have examined the effects of spm and dacB mutations and sporulation temperature on spore core water content and spore resistance to moist heat, UV radiation, and a number of chemicals. The results of these analyses indicate that for C. perfringens SM101 (i) core water content and, probably, cortex PG structure have little if any role in spore resistance to UV and formaldehyde, presumably because these spores' DNA is saturated with α/β-type SASP; (ii) spore resistance to moist heat and nitrous acid is determined to a large extent by core water content and, probably, cortex structure; (iii) core water content and cortex PG cross-linking play little or no role in spore resistance to hydrogen peroxide; (iv) spore core water content decreases with higher sporulation temperatures, resulting in spores that are more resistant to moist heat; and (v) factors in addition to SpmAB, DacB, and sporulation temperature play roles in determining spore core water content and thus, spore resistance to moist heat.

Idioma originalEnglish
Páginas (desde-hasta)3730-3738
Número de páginas9
PublicaciónApplied and Environmental Microbiology
Volumen74
N.º12
DOI
EstadoPublished - jun 2008

Huella dactilar

Clostridium perfringens
Spores
ultraviolet radiation
spore
spores
Hot Temperature
Radiation
heat
protein
sporulation
Proteins
proteins
water content
Water
Peptidoglycan
peptidoglycans
cortex
Temperature
radiation
chemical

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Science(all)
  • Microbiology
  • Ecology
  • Food Science
  • Applied Microbiology and Biotechnology

Citar esto

Paredes-Sabja, Daniel ; Sarker, Nahid ; Setlow, Barbara ; Setlow, Peter ; Sarker, Mahfuzur R. / Roles of DacB and Spm proteins in Clostridium perfringens spore resistance to moist heat, chemicals, and UV radiation. En: Applied and Environmental Microbiology. 2008 ; Vol. 74, N.º 12. pp. 3730-3738.
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title = "Roles of DacB and Spm proteins in Clostridium perfringens spore resistance to moist heat, chemicals, and UV radiation",
abstract = "Clostridium perfringens food poisoning is caused mainly by enterotoxigenic type A isolates that typically possess high spore heat resistance. Previous studies have shown that α/β-type small, acid-soluble proteins (SASP) play a major role in the resistance of Bacillus subtilis and C. perfringens spores to moist heat, UV radiation, and some chemicals. Additional major factors in B. subtilis spore resistance are the spore's core water content and cortex peptidoglycan (PG) structure, with the latter properties modulated by the spm and dacB gene products and the sporulation temperature. In the current work, we have shown that the spm and dacB genes are expressed only during C. perfringens sporulation and have examined the effects of spm and dacB mutations and sporulation temperature on spore core water content and spore resistance to moist heat, UV radiation, and a number of chemicals. The results of these analyses indicate that for C. perfringens SM101 (i) core water content and, probably, cortex PG structure have little if any role in spore resistance to UV and formaldehyde, presumably because these spores' DNA is saturated with α/β-type SASP; (ii) spore resistance to moist heat and nitrous acid is determined to a large extent by core water content and, probably, cortex structure; (iii) core water content and cortex PG cross-linking play little or no role in spore resistance to hydrogen peroxide; (iv) spore core water content decreases with higher sporulation temperatures, resulting in spores that are more resistant to moist heat; and (v) factors in addition to SpmAB, DacB, and sporulation temperature play roles in determining spore core water content and thus, spore resistance to moist heat.",
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Roles of DacB and Spm proteins in Clostridium perfringens spore resistance to moist heat, chemicals, and UV radiation. / Paredes-Sabja, Daniel; Sarker, Nahid; Setlow, Barbara; Setlow, Peter; Sarker, Mahfuzur R.

En: Applied and Environmental Microbiology, Vol. 74, N.º 12, 06.2008, p. 3730-3738.

Resultado de la investigación: Article

TY - JOUR

T1 - Roles of DacB and Spm proteins in Clostridium perfringens spore resistance to moist heat, chemicals, and UV radiation

AU - Paredes-Sabja, Daniel

AU - Sarker, Nahid

AU - Setlow, Barbara

AU - Setlow, Peter

AU - Sarker, Mahfuzur R.

PY - 2008/6

Y1 - 2008/6

N2 - Clostridium perfringens food poisoning is caused mainly by enterotoxigenic type A isolates that typically possess high spore heat resistance. Previous studies have shown that α/β-type small, acid-soluble proteins (SASP) play a major role in the resistance of Bacillus subtilis and C. perfringens spores to moist heat, UV radiation, and some chemicals. Additional major factors in B. subtilis spore resistance are the spore's core water content and cortex peptidoglycan (PG) structure, with the latter properties modulated by the spm and dacB gene products and the sporulation temperature. In the current work, we have shown that the spm and dacB genes are expressed only during C. perfringens sporulation and have examined the effects of spm and dacB mutations and sporulation temperature on spore core water content and spore resistance to moist heat, UV radiation, and a number of chemicals. The results of these analyses indicate that for C. perfringens SM101 (i) core water content and, probably, cortex PG structure have little if any role in spore resistance to UV and formaldehyde, presumably because these spores' DNA is saturated with α/β-type SASP; (ii) spore resistance to moist heat and nitrous acid is determined to a large extent by core water content and, probably, cortex structure; (iii) core water content and cortex PG cross-linking play little or no role in spore resistance to hydrogen peroxide; (iv) spore core water content decreases with higher sporulation temperatures, resulting in spores that are more resistant to moist heat; and (v) factors in addition to SpmAB, DacB, and sporulation temperature play roles in determining spore core water content and thus, spore resistance to moist heat.

AB - Clostridium perfringens food poisoning is caused mainly by enterotoxigenic type A isolates that typically possess high spore heat resistance. Previous studies have shown that α/β-type small, acid-soluble proteins (SASP) play a major role in the resistance of Bacillus subtilis and C. perfringens spores to moist heat, UV radiation, and some chemicals. Additional major factors in B. subtilis spore resistance are the spore's core water content and cortex peptidoglycan (PG) structure, with the latter properties modulated by the spm and dacB gene products and the sporulation temperature. In the current work, we have shown that the spm and dacB genes are expressed only during C. perfringens sporulation and have examined the effects of spm and dacB mutations and sporulation temperature on spore core water content and spore resistance to moist heat, UV radiation, and a number of chemicals. The results of these analyses indicate that for C. perfringens SM101 (i) core water content and, probably, cortex PG structure have little if any role in spore resistance to UV and formaldehyde, presumably because these spores' DNA is saturated with α/β-type SASP; (ii) spore resistance to moist heat and nitrous acid is determined to a large extent by core water content and, probably, cortex structure; (iii) core water content and cortex PG cross-linking play little or no role in spore resistance to hydrogen peroxide; (iv) spore core water content decreases with higher sporulation temperatures, resulting in spores that are more resistant to moist heat; and (v) factors in addition to SpmAB, DacB, and sporulation temperature play roles in determining spore core water content and thus, spore resistance to moist heat.

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U2 - 10.1128/AEM.00169-08

DO - 10.1128/AEM.00169-08

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JO - Applied and Environmental Microbiology

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