TY - JOUR
T1 - Role of the ubiquitin-proteasome system in the sarcopenic-like phenotype induced by CCL5/RANTES
AU - Conejeros-Lillo, Sabrina
AU - Aguirre, Francisco
AU - Cabrera, Daniel
AU - Simon, Felipe
AU - Peñailillo, Luis
AU - Cabello-Verrugio, Claudio
N1 - Publisher Copyright:
© 2024 Page Press Publications. All rights reserved.
PY - 2024
Y1 - 2024
N2 - Sarcopenia is characterized by reduced muscle strength and mass, and a decline in muscle fiber diameter and amount of sarcomeric proteins. Sarcopenia involves the activation of the ubiquitin-proteasome system (UPS). MuRF-1 and atrogin-1 are E3 ubiquitin ligases belonging to UPS, leading to proteolysis mediated by the PSMB 5, 6, and 7 subunits of 20S proteasome. CCL5/RANTES induces a sarcopenic-like effect in muscle cells. The present work explored the impact of CCL5 on UPS components and the influence of UPS on its sarcopenic-like effect. We demonstrated that CCL5 increased MuRF-1 and atrogin-1 protein levels and mRNA levels of subunits PSMB 5, 6, and 7. We used the MG132 inhibitor to elucidate the role of the 20S proteasome in the CCL5-induced sarcopenic-like effect. This inhibitor prevented the decrease in troponin and MHC protein levels and partially stopped the reduction in the diameter of single-isolated flexor digitorum brevis (FDB) muscle fibers induced by CCL5. These findings indicate that CCL5 actively modulates the UPS. Moreover, our results show the direct participation of UPS in the sarcopenic-like phenotype induced by CCL5.
AB - Sarcopenia is characterized by reduced muscle strength and mass, and a decline in muscle fiber diameter and amount of sarcomeric proteins. Sarcopenia involves the activation of the ubiquitin-proteasome system (UPS). MuRF-1 and atrogin-1 are E3 ubiquitin ligases belonging to UPS, leading to proteolysis mediated by the PSMB 5, 6, and 7 subunits of 20S proteasome. CCL5/RANTES induces a sarcopenic-like effect in muscle cells. The present work explored the impact of CCL5 on UPS components and the influence of UPS on its sarcopenic-like effect. We demonstrated that CCL5 increased MuRF-1 and atrogin-1 protein levels and mRNA levels of subunits PSMB 5, 6, and 7. We used the MG132 inhibitor to elucidate the role of the 20S proteasome in the CCL5-induced sarcopenic-like effect. This inhibitor prevented the decrease in troponin and MHC protein levels and partially stopped the reduction in the diameter of single-isolated flexor digitorum brevis (FDB) muscle fibers induced by CCL5. These findings indicate that CCL5 actively modulates the UPS. Moreover, our results show the direct participation of UPS in the sarcopenic-like phenotype induced by CCL5.
KW - Irisin
KW - muscle
KW - physical exercise
KW - saliva
KW - serum
UR - http://www.scopus.com/inward/record.url?scp=85191055342&partnerID=8YFLogxK
U2 - 10.4081/ejtm.2024.12249
DO - 10.4081/ejtm.2024.12249
M3 - Article
AN - SCOPUS:85191055342
SN - 2037-7452
VL - 34
JO - European Journal of Translational Myology
JF - European Journal of Translational Myology
IS - 1
ER -