Role of Salmonella Typhimurium small RNAs RyhB-1 and RyhB-2 in the oxidative stress response

Iván L. Calderón, Eduardo H. Morales, Bernardo Collao, Paulina F. Calderón, Catalina A. Chahuán, Lillian G. Acuña, Fernando Gil, Claudia P. Saavedra

Resultado de la investigación: Article

25 Citas (Scopus)

Resumen

As part of the response to specific stress conditions, bacteria express small molecules of non-coding RNA which maintain cellular homeostasis by regulating gene expression, commonly at the post-transcriptional level. Among these, in Salmonella enterica sv. Typhimurium, the paralog small non-coding RNAs RyhB-1 and RyhB-2 play an important role in iron homeostasis. In addition, in the present work, we show that RyhB-1 and RyhB-2 also participate in the response to hydrogen peroxide (H2O2). Deletion of RyhB-1 and/or RyhB-2 resulted in increased levels of intracellular reactive oxygen species, protein carbonylation and an altered NADH/NAD+ ratio. Analyses of the transcriptional profiles of ryhB-1 and ryhB-2 by northern blot and qRT-PCR showed that they are induced in response to H2O2 in an OxyR-dependent manner. By using lacZ-fusions and electrophoretic mobility shift assays, we confirmed the requirement of OxyR for inducing expression of both ryhB-1 and ryhB-2. Taken together, our results support a model in which, in response to peroxide treatment, ryhB-1 and ryhB-2 are upregulated by OxyR through direct interaction with their promoter region.

Idioma originalEnglish
Páginas (desde-hasta)30-40
Número de páginas11
PublicaciónResearch in Microbiology
Volumen165
N.º1
DOI
EstadoPublished - 1 ene 2014

Huella dactilar

Small Untranslated RNA
Salmonella typhimurium
NAD
Oxidative Stress
Homeostasis
Protein Carbonylation
RNA
Salmonella enterica
Peroxides
Electrophoretic Mobility Shift Assay
Genetic Promoter Regions
Northern Blotting
Hydrogen Peroxide
Reactive Oxygen Species
Iron
Bacteria
Gene Expression
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Citar esto

Calderón, Iván L. ; Morales, Eduardo H. ; Collao, Bernardo ; Calderón, Paulina F. ; Chahuán, Catalina A. ; Acuña, Lillian G. ; Gil, Fernando ; Saavedra, Claudia P. / Role of Salmonella Typhimurium small RNAs RyhB-1 and RyhB-2 in the oxidative stress response. En: Research in Microbiology. 2014 ; Vol. 165, N.º 1. pp. 30-40.
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title = "Role of Salmonella Typhimurium small RNAs RyhB-1 and RyhB-2 in the oxidative stress response",
abstract = "As part of the response to specific stress conditions, bacteria express small molecules of non-coding RNA which maintain cellular homeostasis by regulating gene expression, commonly at the post-transcriptional level. Among these, in Salmonella enterica sv. Typhimurium, the paralog small non-coding RNAs RyhB-1 and RyhB-2 play an important role in iron homeostasis. In addition, in the present work, we show that RyhB-1 and RyhB-2 also participate in the response to hydrogen peroxide (H2O2). Deletion of RyhB-1 and/or RyhB-2 resulted in increased levels of intracellular reactive oxygen species, protein carbonylation and an altered NADH/NAD+ ratio. Analyses of the transcriptional profiles of ryhB-1 and ryhB-2 by northern blot and qRT-PCR showed that they are induced in response to H2O2 in an OxyR-dependent manner. By using lacZ-fusions and electrophoretic mobility shift assays, we confirmed the requirement of OxyR for inducing expression of both ryhB-1 and ryhB-2. Taken together, our results support a model in which, in response to peroxide treatment, ryhB-1 and ryhB-2 are upregulated by OxyR through direct interaction with their promoter region.",
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Role of Salmonella Typhimurium small RNAs RyhB-1 and RyhB-2 in the oxidative stress response. / Calderón, Iván L.; Morales, Eduardo H.; Collao, Bernardo; Calderón, Paulina F.; Chahuán, Catalina A.; Acuña, Lillian G.; Gil, Fernando; Saavedra, Claudia P.

En: Research in Microbiology, Vol. 165, N.º 1, 01.01.2014, p. 30-40.

Resultado de la investigación: Article

TY - JOUR

T1 - Role of Salmonella Typhimurium small RNAs RyhB-1 and RyhB-2 in the oxidative stress response

AU - Calderón, Iván L.

AU - Morales, Eduardo H.

AU - Collao, Bernardo

AU - Calderón, Paulina F.

AU - Chahuán, Catalina A.

AU - Acuña, Lillian G.

AU - Gil, Fernando

AU - Saavedra, Claudia P.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - As part of the response to specific stress conditions, bacteria express small molecules of non-coding RNA which maintain cellular homeostasis by regulating gene expression, commonly at the post-transcriptional level. Among these, in Salmonella enterica sv. Typhimurium, the paralog small non-coding RNAs RyhB-1 and RyhB-2 play an important role in iron homeostasis. In addition, in the present work, we show that RyhB-1 and RyhB-2 also participate in the response to hydrogen peroxide (H2O2). Deletion of RyhB-1 and/or RyhB-2 resulted in increased levels of intracellular reactive oxygen species, protein carbonylation and an altered NADH/NAD+ ratio. Analyses of the transcriptional profiles of ryhB-1 and ryhB-2 by northern blot and qRT-PCR showed that they are induced in response to H2O2 in an OxyR-dependent manner. By using lacZ-fusions and electrophoretic mobility shift assays, we confirmed the requirement of OxyR for inducing expression of both ryhB-1 and ryhB-2. Taken together, our results support a model in which, in response to peroxide treatment, ryhB-1 and ryhB-2 are upregulated by OxyR through direct interaction with their promoter region.

AB - As part of the response to specific stress conditions, bacteria express small molecules of non-coding RNA which maintain cellular homeostasis by regulating gene expression, commonly at the post-transcriptional level. Among these, in Salmonella enterica sv. Typhimurium, the paralog small non-coding RNAs RyhB-1 and RyhB-2 play an important role in iron homeostasis. In addition, in the present work, we show that RyhB-1 and RyhB-2 also participate in the response to hydrogen peroxide (H2O2). Deletion of RyhB-1 and/or RyhB-2 resulted in increased levels of intracellular reactive oxygen species, protein carbonylation and an altered NADH/NAD+ ratio. Analyses of the transcriptional profiles of ryhB-1 and ryhB-2 by northern blot and qRT-PCR showed that they are induced in response to H2O2 in an OxyR-dependent manner. By using lacZ-fusions and electrophoretic mobility shift assays, we confirmed the requirement of OxyR for inducing expression of both ryhB-1 and ryhB-2. Taken together, our results support a model in which, in response to peroxide treatment, ryhB-1 and ryhB-2 are upregulated by OxyR through direct interaction with their promoter region.

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