Role of GerKB in germination and outgrowth of Clostridium perfringens spores

Daniel Paredes-Sabja, Peter Setlow, Mahfuzur R. Sarker

Resultado de la investigación: Article

32 Citas (Scopus)

Resumen

Previous work indicated that Clostridium perfringens gerKA gerKC spores germinate significantly, suggesting that gerKB also has a role in C. perfringens spore germination. We now find that (i) gerKB was expressed only during sporulation, likely in the forespore; (ii) gerKB spores germinated like wild-type spores with nonnutrient germinants and with high concentrations of nutrients but more slowly with low nutrient concentrations; and (iii) gerKB spores had lower colony-forming efficiency and slower outgrowth than wild-type spores. These results suggest that GerKB plays an auxiliary role in spore germination under some conditions and is required for normal spore viability and outgrowth.

Idioma originalEnglish
Páginas (desde-hasta)3813-3817
Número de páginas5
PublicaciónApplied and Environmental Microbiology
Volumen75
N.º11
DOI
EstadoPublished - jun 2009

Huella dactilar

Clostridium perfringens
Germination
Spores
spore
germination
spores
spore germination
nutrient content
sporulation
Food
nutrient
viability

ASJC Scopus subject areas

  • Food Science
  • Biotechnology
  • Ecology
  • Applied Microbiology and Biotechnology

Citar esto

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abstract = "Previous work indicated that Clostridium perfringens gerKA gerKC spores germinate significantly, suggesting that gerKB also has a role in C. perfringens spore germination. We now find that (i) gerKB was expressed only during sporulation, likely in the forespore; (ii) gerKB spores germinated like wild-type spores with nonnutrient germinants and with high concentrations of nutrients but more slowly with low nutrient concentrations; and (iii) gerKB spores had lower colony-forming efficiency and slower outgrowth than wild-type spores. These results suggest that GerKB plays an auxiliary role in spore germination under some conditions and is required for normal spore viability and outgrowth.",
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Role of GerKB in germination and outgrowth of Clostridium perfringens spores. / Paredes-Sabja, Daniel; Setlow, Peter; Sarker, Mahfuzur R.

En: Applied and Environmental Microbiology, Vol. 75, N.º 11, 06.2009, p. 3813-3817.

Resultado de la investigación: Article

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AU - Setlow, Peter

AU - Sarker, Mahfuzur R.

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