Ribosomal RNA genes of Saccharomyces cerevisiae. II. Physical map and nucleotide sequence of the 5 S ribosomal RNA gene and adjacent intergenic regions

P. Valenzuela, G. I. Bell, A. Venegas, E. T. Sewell, F. R. Masiarz, L. J. DeGennaro, F. Weinberg, W. J. Rutter

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

44 Citas (Scopus)

Resumen

A DNA fragment containing the structural gene for the 5 S ribosomal RNA and intergenic regions before and after the 35 S ribosomal RNA precursor gene of Saccharomyces cerevisiae has been amplified in a bacterial plasmid and physically mapped by restriction endonuclease cleavage and hybridization to purified yeast 5 S ribosomal RNA. The nucleotide sequence of the DNA fragments carrying the 5 S ribosomal RNA gene and adjacent regions has been determined. The sequence unambiguously identifies the 5 S ribosomal RNA gene, determines its polarity within the ribosomal DNA repeating unit, and reveals the structure of its promoter and termination regions. Partial DNA sequence of the regions near the beginning and end of the 35 S ribosomal RNA gene has also been determined as a preliminary step in establishing the structure of promoter and termination regions for the 35 S ribosomal RNA gene.

Idioma originalInglés
Páginas (desde-hasta)8126-8135
Número de páginas10
PublicaciónJournal of Biological Chemistry
Volumen252
N.º22
EstadoPublicada - 1977

Áreas temáticas de ASJC Scopus

  • Bioquímica
  • Biología molecular
  • Biología celular

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