Ribosomal RNA genes of Saccharomyces cerevisiae. I. Physical map of the repeating unit and location of the regions coding for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs

G. I. Bell, L. J. DeGennaro, D. H. Gelfand, R. J. Bishop, P. Valenzuela, W. J. Rutter

Resultado de la investigación: Article

141 Citas (Scopus)

Resumen

The organization of the ribosomal DNA repeating unit from Saccharomyces cerevisiae has been analyzed. A cloned ribosomal DNA repeating unit has been mapped with the restriction enzymes Xma I, Kpn I, HindIII, Xba I, Bgl I + II, and Eco RI. The locations of the sequences which code for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs have been determined by hybridization of the purified RNA species with restriction endonuclease generated fragments of the repeating unit. The position of the 5.8 S ribosomal DNA sequences within the repeat was also established by sequencing the DNA which codes for 83 nucleotides at the 5' end of 5.8 S ribosomal RNA. The polarity of the 35 S ribosomal RNA precursor has been established by a combination of hybridization analysis and DNA sequence determination and is 5'-18 S, 5.8 S, 25 S-3'.

Idioma originalEnglish
Páginas (desde-hasta)8118-8125
Número de páginas8
PublicaciónJournal of Biological Chemistry
Volumen252
N.º22
EstadoPublished - 1977

Huella dactilar

Ribosomal RNA
Ribosomal DNA
rRNA Genes
Yeast
Saccharomyces cerevisiae
Genes
DNA sequences
DNA Sequence Analysis
Deoxyribonuclease EcoRI
RNA Precursors
DNA Restriction Enzymes
Nucleotides
RNA
DNA
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Citar esto

Bell, G. I. ; DeGennaro, L. J. ; Gelfand, D. H. ; Bishop, R. J. ; Valenzuela, P. ; Rutter, W. J. / Ribosomal RNA genes of Saccharomyces cerevisiae. I. Physical map of the repeating unit and location of the regions coding for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs. En: Journal of Biological Chemistry. 1977 ; Vol. 252, N.º 22. pp. 8118-8125.
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abstract = "The organization of the ribosomal DNA repeating unit from Saccharomyces cerevisiae has been analyzed. A cloned ribosomal DNA repeating unit has been mapped with the restriction enzymes Xma I, Kpn I, HindIII, Xba I, Bgl I + II, and Eco RI. The locations of the sequences which code for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs have been determined by hybridization of the purified RNA species with restriction endonuclease generated fragments of the repeating unit. The position of the 5.8 S ribosomal DNA sequences within the repeat was also established by sequencing the DNA which codes for 83 nucleotides at the 5' end of 5.8 S ribosomal RNA. The polarity of the 35 S ribosomal RNA precursor has been established by a combination of hybridization analysis and DNA sequence determination and is 5'-18 S, 5.8 S, 25 S-3'.",
author = "Bell, {G. I.} and DeGennaro, {L. J.} and Gelfand, {D. H.} and Bishop, {R. J.} and P. Valenzuela and Rutter, {W. J.}",
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Ribosomal RNA genes of Saccharomyces cerevisiae. I. Physical map of the repeating unit and location of the regions coding for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs. / Bell, G. I.; DeGennaro, L. J.; Gelfand, D. H.; Bishop, R. J.; Valenzuela, P.; Rutter, W. J.

En: Journal of Biological Chemistry, Vol. 252, N.º 22, 1977, p. 8118-8125.

Resultado de la investigación: Article

TY - JOUR

T1 - Ribosomal RNA genes of Saccharomyces cerevisiae. I. Physical map of the repeating unit and location of the regions coding for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs

AU - Bell, G. I.

AU - DeGennaro, L. J.

AU - Gelfand, D. H.

AU - Bishop, R. J.

AU - Valenzuela, P.

AU - Rutter, W. J.

PY - 1977

Y1 - 1977

N2 - The organization of the ribosomal DNA repeating unit from Saccharomyces cerevisiae has been analyzed. A cloned ribosomal DNA repeating unit has been mapped with the restriction enzymes Xma I, Kpn I, HindIII, Xba I, Bgl I + II, and Eco RI. The locations of the sequences which code for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs have been determined by hybridization of the purified RNA species with restriction endonuclease generated fragments of the repeating unit. The position of the 5.8 S ribosomal DNA sequences within the repeat was also established by sequencing the DNA which codes for 83 nucleotides at the 5' end of 5.8 S ribosomal RNA. The polarity of the 35 S ribosomal RNA precursor has been established by a combination of hybridization analysis and DNA sequence determination and is 5'-18 S, 5.8 S, 25 S-3'.

AB - The organization of the ribosomal DNA repeating unit from Saccharomyces cerevisiae has been analyzed. A cloned ribosomal DNA repeating unit has been mapped with the restriction enzymes Xma I, Kpn I, HindIII, Xba I, Bgl I + II, and Eco RI. The locations of the sequences which code for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs have been determined by hybridization of the purified RNA species with restriction endonuclease generated fragments of the repeating unit. The position of the 5.8 S ribosomal DNA sequences within the repeat was also established by sequencing the DNA which codes for 83 nucleotides at the 5' end of 5.8 S ribosomal RNA. The polarity of the 35 S ribosomal RNA precursor has been established by a combination of hybridization analysis and DNA sequence determination and is 5'-18 S, 5.8 S, 25 S-3'.

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