Responses in bacterial community structure to waste nutrients from aquaculture: An in situ microcosm experiment in a Chilean fjord

Lasse Mork Olsen, Klaudia L. Hernández, Murat Van Ardelan, Jose Luis Iriarte, K. Can Bizsel, Yngvar Olsen

Resultado de la investigación: Article

6 Citas (Scopus)

Resumen

Chilean salmon farms release inorganic nutrients excreted by the fish into the surrounding water in Patagonian fjords. The objective of this experiment from the Comau Fjord (42.2° S) in southern Chile was to study how increased input of ammonium (NH4) and phosphate (PO4) from salmon farms might affect the community structure of bacteria in surface waters where fish farms are located. We used microcosms (35 l) with NH4-N and PO4-P added to the natural seawater in a gradient of nutrient-loading rates, with the same N:P ratio as in salmon aquaculture effluents. Additionally, we measured bacterial community structure at different depths in the Comau Fjord to assess the natural variation to compare with our experiment. We used denaturing gradient gel electrophoresis (DGGE) to create 16S rDNA fingerprints of the bacterial communities and monitored biological and environmental variables (chlorophyll a, inorganic nutrients, pH, microbial abundance). The nutrient-loading rate had a significant impact on the bacterial community structure, and the community dissimilarity between low and high nutrient additions was up to 78%. Of the measured environmental variables, phytoplankton abundance and increased pH from photosynthesis had a significant effect. We observed no significant changes in bacterial diversity, which remained at the same level as in the unmanipulated community. Thus, the bac - terial community of the fjord was not resistant, but resilient within the time frame and nutrient gradient of our experiment.

Idioma originalEnglish
Páginas (desde-hasta)21-32
Número de páginas12
PublicaciónAquaculture Environment Interactions
Volumen9
N.º1
DOI
EstadoPublished - 2017

Huella dactilar

fjord
bacterial communities
microcosm
aquaculture
community structure
salmon
nutrient
nutrients
pollution load
experiment
farm
farms
environmental factors
fish farms
denaturing gradient gel electrophoresis
aquaculture effluent
effluents
Chile
surface water
seawater

ASJC Scopus subject areas

  • Aquatic Science
  • Water Science and Technology
  • Management, Monitoring, Policy and Law

Citar esto

Olsen, Lasse Mork ; Hernández, Klaudia L. ; Ardelan, Murat Van ; Iriarte, Jose Luis ; Bizsel, K. Can ; Olsen, Yngvar. / Responses in bacterial community structure to waste nutrients from aquaculture : An in situ microcosm experiment in a Chilean fjord. En: Aquaculture Environment Interactions. 2017 ; Vol. 9, N.º 1. pp. 21-32.
@article{4b83e733ea5f48999563cbc1fa13eecf,
title = "Responses in bacterial community structure to waste nutrients from aquaculture: An in situ microcosm experiment in a Chilean fjord",
abstract = "Chilean salmon farms release inorganic nutrients excreted by the fish into the surrounding water in Patagonian fjords. The objective of this experiment from the Comau Fjord (42.2° S) in southern Chile was to study how increased input of ammonium (NH4) and phosphate (PO4) from salmon farms might affect the community structure of bacteria in surface waters where fish farms are located. We used microcosms (35 l) with NH4-N and PO4-P added to the natural seawater in a gradient of nutrient-loading rates, with the same N:P ratio as in salmon aquaculture effluents. Additionally, we measured bacterial community structure at different depths in the Comau Fjord to assess the natural variation to compare with our experiment. We used denaturing gradient gel electrophoresis (DGGE) to create 16S rDNA fingerprints of the bacterial communities and monitored biological and environmental variables (chlorophyll a, inorganic nutrients, pH, microbial abundance). The nutrient-loading rate had a significant impact on the bacterial community structure, and the community dissimilarity between low and high nutrient additions was up to 78{\%}. Of the measured environmental variables, phytoplankton abundance and increased pH from photosynthesis had a significant effect. We observed no significant changes in bacterial diversity, which remained at the same level as in the unmanipulated community. Thus, the bac - terial community of the fjord was not resistant, but resilient within the time frame and nutrient gradient of our experiment.",
keywords = "Aquaculture, Bacterial diversity, Chile, Eutrophication, Microbial community, Patagonia",
author = "Olsen, {Lasse Mork} and Hern{\'a}ndez, {Klaudia L.} and Ardelan, {Murat Van} and Iriarte, {Jose Luis} and Bizsel, {K. Can} and Yngvar Olsen",
year = "2017",
doi = "10.3354/aei00212",
language = "English",
volume = "9",
pages = "21--32",
journal = "Aquaculture Environment Interactions",
issn = "1869-215X",
publisher = "Inter-Research",
number = "1",

}

Responses in bacterial community structure to waste nutrients from aquaculture : An in situ microcosm experiment in a Chilean fjord. / Olsen, Lasse Mork; Hernández, Klaudia L.; Ardelan, Murat Van; Iriarte, Jose Luis; Bizsel, K. Can; Olsen, Yngvar.

En: Aquaculture Environment Interactions, Vol. 9, N.º 1, 2017, p. 21-32.

Resultado de la investigación: Article

TY - JOUR

T1 - Responses in bacterial community structure to waste nutrients from aquaculture

T2 - An in situ microcosm experiment in a Chilean fjord

AU - Olsen, Lasse Mork

AU - Hernández, Klaudia L.

AU - Ardelan, Murat Van

AU - Iriarte, Jose Luis

AU - Bizsel, K. Can

AU - Olsen, Yngvar

PY - 2017

Y1 - 2017

N2 - Chilean salmon farms release inorganic nutrients excreted by the fish into the surrounding water in Patagonian fjords. The objective of this experiment from the Comau Fjord (42.2° S) in southern Chile was to study how increased input of ammonium (NH4) and phosphate (PO4) from salmon farms might affect the community structure of bacteria in surface waters where fish farms are located. We used microcosms (35 l) with NH4-N and PO4-P added to the natural seawater in a gradient of nutrient-loading rates, with the same N:P ratio as in salmon aquaculture effluents. Additionally, we measured bacterial community structure at different depths in the Comau Fjord to assess the natural variation to compare with our experiment. We used denaturing gradient gel electrophoresis (DGGE) to create 16S rDNA fingerprints of the bacterial communities and monitored biological and environmental variables (chlorophyll a, inorganic nutrients, pH, microbial abundance). The nutrient-loading rate had a significant impact on the bacterial community structure, and the community dissimilarity between low and high nutrient additions was up to 78%. Of the measured environmental variables, phytoplankton abundance and increased pH from photosynthesis had a significant effect. We observed no significant changes in bacterial diversity, which remained at the same level as in the unmanipulated community. Thus, the bac - terial community of the fjord was not resistant, but resilient within the time frame and nutrient gradient of our experiment.

AB - Chilean salmon farms release inorganic nutrients excreted by the fish into the surrounding water in Patagonian fjords. The objective of this experiment from the Comau Fjord (42.2° S) in southern Chile was to study how increased input of ammonium (NH4) and phosphate (PO4) from salmon farms might affect the community structure of bacteria in surface waters where fish farms are located. We used microcosms (35 l) with NH4-N and PO4-P added to the natural seawater in a gradient of nutrient-loading rates, with the same N:P ratio as in salmon aquaculture effluents. Additionally, we measured bacterial community structure at different depths in the Comau Fjord to assess the natural variation to compare with our experiment. We used denaturing gradient gel electrophoresis (DGGE) to create 16S rDNA fingerprints of the bacterial communities and monitored biological and environmental variables (chlorophyll a, inorganic nutrients, pH, microbial abundance). The nutrient-loading rate had a significant impact on the bacterial community structure, and the community dissimilarity between low and high nutrient additions was up to 78%. Of the measured environmental variables, phytoplankton abundance and increased pH from photosynthesis had a significant effect. We observed no significant changes in bacterial diversity, which remained at the same level as in the unmanipulated community. Thus, the bac - terial community of the fjord was not resistant, but resilient within the time frame and nutrient gradient of our experiment.

KW - Aquaculture

KW - Bacterial diversity

KW - Chile

KW - Eutrophication

KW - Microbial community

KW - Patagonia

UR - http://www.scopus.com/inward/record.url?scp=85017276283&partnerID=8YFLogxK

U2 - 10.3354/aei00212

DO - 10.3354/aei00212

M3 - Article

AN - SCOPUS:85017276283

VL - 9

SP - 21

EP - 32

JO - Aquaculture Environment Interactions

JF - Aquaculture Environment Interactions

SN - 1869-215X

IS - 1

ER -