Removal of the endothelial layer in perfused mesenteric vascular bed of the rat

Teresa Pelissier, Hugo F. Miranda, Diego Bustamante, Carlos Paeile, Gianni Pinardi

Resultado de la investigación: Article

5 Citas (Scopus)

Resumen

The endothelial layer was removed from the isolated mesenteric vascular bed of the rat by perfusion with hypotonic Tyrode solution for 12.5 min. This procedure damaged more than 95% of the endothelial cells. After endothelial removal, the response to norepinephrine was significantly enhanced, whereas the relaxation induced by acetylcholine (ACh) was completely abolished. The results of this work show that perfusion with hypotonic solutions provides a reliable method of endothelial removal in isolated perfused vascular beds, allowing the study of endothelial-dependent vascular responses.

Idioma originalEnglish
Páginas (desde-hasta)41-44
Número de páginas4
PublicaciónJournal of Pharmacological and Toxicological Methods
Volumen27
N.º1
DOI
EstadoPublished - 1992

Huella dactilar

Hypotonic Solutions
Blood Vessels
Rats
Endothelial cells
Perfusion
Acetylcholine
Norepinephrine
Endothelial Cells
Tyrode's solution

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology

Citar esto

Pelissier, Teresa ; Miranda, Hugo F. ; Bustamante, Diego ; Paeile, Carlos ; Pinardi, Gianni. / Removal of the endothelial layer in perfused mesenteric vascular bed of the rat. En: Journal of Pharmacological and Toxicological Methods. 1992 ; Vol. 27, N.º 1. pp. 41-44.
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Removal of the endothelial layer in perfused mesenteric vascular bed of the rat. / Pelissier, Teresa; Miranda, Hugo F.; Bustamante, Diego; Paeile, Carlos; Pinardi, Gianni.

En: Journal of Pharmacological and Toxicological Methods, Vol. 27, N.º 1, 1992, p. 41-44.

Resultado de la investigación: Article

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AU - Miranda, Hugo F.

AU - Bustamante, Diego

AU - Paeile, Carlos

AU - Pinardi, Gianni

PY - 1992

Y1 - 1992

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AB - The endothelial layer was removed from the isolated mesenteric vascular bed of the rat by perfusion with hypotonic Tyrode solution for 12.5 min. This procedure damaged more than 95% of the endothelial cells. After endothelial removal, the response to norepinephrine was significantly enhanced, whereas the relaxation induced by acetylcholine (ACh) was completely abolished. The results of this work show that perfusion with hypotonic solutions provides a reliable method of endothelial removal in isolated perfused vascular beds, allowing the study of endothelial-dependent vascular responses.

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KW - Endothelium

KW - Mesenteric vascular bed

KW - Norepinephrine

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