TY - JOUR
T1 - Regulation of inhibin/activin expression in rat early antral follicles
AU - Andreone, Luz
AU - Velásquez, Ethel V.
AU - Abramovich, Dalhia
AU - Ambao, Verónica
AU - Loreti, Nazareth
AU - Croxatto, Horacio B.
AU - Parborell, Fernanda
AU - Tesone, Marta
AU - Campo, Stella
N1 - Funding Information:
The authors are very grateful to NIDDK-NIH's National Hormone & Peptide Program and A.F. Parlow for supplying rhFSH. This work was supported by grants from the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET, PIP 5479 to SC and PIP 5471 to MT), Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT, PICT 25365 to SC and PICT 06384 to MT) and Universidad de Buenos Aires (UBA, X237 to MT).
PY - 2009/10/15
Y1 - 2009/10/15
N2 - The aim of the present study was to determine the endocrine activity of cultured early antral follicles (EAF) isolated from prepubertal diethylstilbestrol-treated rats. The effect of steroidogenic substrates and FSH on steroid, inhibin A and B, Pro-αC and activin A production was evaluated. Androsterone was the predominant steroid produced by EAF. The addition of androstenedione, androstenedione + FSH and progesterone stimulated oestradiol production, whereas 25-hydroxycholesterol (25-OH-Chol) increased progesterone production. Inhibin A, B, Pro-αC, and activin A were produced under basal conditions. The predominance of inhibin B over inhibin A was not affected by the addition of androstenedione or progesterone. Inhibin A and activin A production was stimulated by FSH. 25-OH-Chol increased Inha, Inhba and Inhbb mRNA expression and the production of the three molecular forms of inhibins but decreased activin A production. These results show that FSH and the steroid follicular microenvironment differentially modulate the gene expression of inhibin/activin subunits, their assembly and secretion.
AB - The aim of the present study was to determine the endocrine activity of cultured early antral follicles (EAF) isolated from prepubertal diethylstilbestrol-treated rats. The effect of steroidogenic substrates and FSH on steroid, inhibin A and B, Pro-αC and activin A production was evaluated. Androsterone was the predominant steroid produced by EAF. The addition of androstenedione, androstenedione + FSH and progesterone stimulated oestradiol production, whereas 25-hydroxycholesterol (25-OH-Chol) increased progesterone production. Inhibin A, B, Pro-αC, and activin A were produced under basal conditions. The predominance of inhibin B over inhibin A was not affected by the addition of androstenedione or progesterone. Inhibin A and activin A production was stimulated by FSH. 25-OH-Chol increased Inha, Inhba and Inhbb mRNA expression and the production of the three molecular forms of inhibins but decreased activin A production. These results show that FSH and the steroid follicular microenvironment differentially modulate the gene expression of inhibin/activin subunits, their assembly and secretion.
KW - 25-Hydroxycholesterol
KW - Activin
KW - Early antral follicles
KW - Inhibin
UR - http://www.scopus.com/inward/record.url?scp=67749146973&partnerID=8YFLogxK
U2 - 10.1016/j.mce.2009.05.003
DO - 10.1016/j.mce.2009.05.003
M3 - Article
C2 - 19464342
AN - SCOPUS:67749146973
VL - 309
SP - 48
EP - 54
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
SN - 0303-7207
IS - 1-2
ER -