Regulation of inhibin/activin expression in rat early antral follicles

Luz Andreone, Ethel V. Velásquez, Dalhia Abramovich, Verónica Ambao, Nazareth Loreti, Horacio B. Croxatto, Fernanda Parborell, Marta Tesone, Stella Campo

Resultado de la investigación: Article

5 Citas (Scopus)

Resumen

The aim of the present study was to determine the endocrine activity of cultured early antral follicles (EAF) isolated from prepubertal diethylstilbestrol-treated rats. The effect of steroidogenic substrates and FSH on steroid, inhibin A and B, Pro-αC and activin A production was evaluated. Androsterone was the predominant steroid produced by EAF. The addition of androstenedione, androstenedione + FSH and progesterone stimulated oestradiol production, whereas 25-hydroxycholesterol (25-OH-Chol) increased progesterone production. Inhibin A, B, Pro-αC, and activin A were produced under basal conditions. The predominance of inhibin B over inhibin A was not affected by the addition of androstenedione or progesterone. Inhibin A and activin A production was stimulated by FSH. 25-OH-Chol increased Inha, Inhba and Inhbb mRNA expression and the production of the three molecular forms of inhibins but decreased activin A production. These results show that FSH and the steroid follicular microenvironment differentially modulate the gene expression of inhibin/activin subunits, their assembly and secretion.

Idioma originalEnglish
Páginas (desde-hasta)48-54
Número de páginas7
PublicaciónMolecular and Cellular Endocrinology
Volumen309
N.º1-2
DOI
EstadoPublished - 15 oct 2009

Huella dactilar

Activins
Inhibins
Rats
Androstenedione
Progesterone
Steroids
Androsterone
Diethylstilbestrol
Estradiol
Gene expression
Antral
activin A
Gene Expression
Messenger RNA
inhibin A
Substrates
inhibin B

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Citar esto

Andreone, L., Velásquez, E. V., Abramovich, D., Ambao, V., Loreti, N., Croxatto, H. B., ... Campo, S. (2009). Regulation of inhibin/activin expression in rat early antral follicles. Molecular and Cellular Endocrinology, 309(1-2), 48-54. https://doi.org/10.1016/j.mce.2009.05.003
Andreone, Luz ; Velásquez, Ethel V. ; Abramovich, Dalhia ; Ambao, Verónica ; Loreti, Nazareth ; Croxatto, Horacio B. ; Parborell, Fernanda ; Tesone, Marta ; Campo, Stella. / Regulation of inhibin/activin expression in rat early antral follicles. En: Molecular and Cellular Endocrinology. 2009 ; Vol. 309, N.º 1-2. pp. 48-54.
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abstract = "The aim of the present study was to determine the endocrine activity of cultured early antral follicles (EAF) isolated from prepubertal diethylstilbestrol-treated rats. The effect of steroidogenic substrates and FSH on steroid, inhibin A and B, Pro-αC and activin A production was evaluated. Androsterone was the predominant steroid produced by EAF. The addition of androstenedione, androstenedione + FSH and progesterone stimulated oestradiol production, whereas 25-hydroxycholesterol (25-OH-Chol) increased progesterone production. Inhibin A, B, Pro-αC, and activin A were produced under basal conditions. The predominance of inhibin B over inhibin A was not affected by the addition of androstenedione or progesterone. Inhibin A and activin A production was stimulated by FSH. 25-OH-Chol increased Inha, Inhba and Inhbb mRNA expression and the production of the three molecular forms of inhibins but decreased activin A production. These results show that FSH and the steroid follicular microenvironment differentially modulate the gene expression of inhibin/activin subunits, their assembly and secretion.",
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Andreone, L, Velásquez, EV, Abramovich, D, Ambao, V, Loreti, N, Croxatto, HB, Parborell, F, Tesone, M & Campo, S 2009, 'Regulation of inhibin/activin expression in rat early antral follicles', Molecular and Cellular Endocrinology, vol. 309, n.º 1-2, pp. 48-54. https://doi.org/10.1016/j.mce.2009.05.003

Regulation of inhibin/activin expression in rat early antral follicles. / Andreone, Luz; Velásquez, Ethel V.; Abramovich, Dalhia; Ambao, Verónica; Loreti, Nazareth; Croxatto, Horacio B.; Parborell, Fernanda; Tesone, Marta; Campo, Stella.

En: Molecular and Cellular Endocrinology, Vol. 309, N.º 1-2, 15.10.2009, p. 48-54.

Resultado de la investigación: Article

TY - JOUR

T1 - Regulation of inhibin/activin expression in rat early antral follicles

AU - Andreone, Luz

AU - Velásquez, Ethel V.

AU - Abramovich, Dalhia

AU - Ambao, Verónica

AU - Loreti, Nazareth

AU - Croxatto, Horacio B.

AU - Parborell, Fernanda

AU - Tesone, Marta

AU - Campo, Stella

PY - 2009/10/15

Y1 - 2009/10/15

N2 - The aim of the present study was to determine the endocrine activity of cultured early antral follicles (EAF) isolated from prepubertal diethylstilbestrol-treated rats. The effect of steroidogenic substrates and FSH on steroid, inhibin A and B, Pro-αC and activin A production was evaluated. Androsterone was the predominant steroid produced by EAF. The addition of androstenedione, androstenedione + FSH and progesterone stimulated oestradiol production, whereas 25-hydroxycholesterol (25-OH-Chol) increased progesterone production. Inhibin A, B, Pro-αC, and activin A were produced under basal conditions. The predominance of inhibin B over inhibin A was not affected by the addition of androstenedione or progesterone. Inhibin A and activin A production was stimulated by FSH. 25-OH-Chol increased Inha, Inhba and Inhbb mRNA expression and the production of the three molecular forms of inhibins but decreased activin A production. These results show that FSH and the steroid follicular microenvironment differentially modulate the gene expression of inhibin/activin subunits, their assembly and secretion.

AB - The aim of the present study was to determine the endocrine activity of cultured early antral follicles (EAF) isolated from prepubertal diethylstilbestrol-treated rats. The effect of steroidogenic substrates and FSH on steroid, inhibin A and B, Pro-αC and activin A production was evaluated. Androsterone was the predominant steroid produced by EAF. The addition of androstenedione, androstenedione + FSH and progesterone stimulated oestradiol production, whereas 25-hydroxycholesterol (25-OH-Chol) increased progesterone production. Inhibin A, B, Pro-αC, and activin A were produced under basal conditions. The predominance of inhibin B over inhibin A was not affected by the addition of androstenedione or progesterone. Inhibin A and activin A production was stimulated by FSH. 25-OH-Chol increased Inha, Inhba and Inhbb mRNA expression and the production of the three molecular forms of inhibins but decreased activin A production. These results show that FSH and the steroid follicular microenvironment differentially modulate the gene expression of inhibin/activin subunits, their assembly and secretion.

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KW - Early antral follicles

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