Regulation of expression of the PetI operon involved in iron oxidation in the biomining bacterium Acidithiobacillus ferrooxidans

C. Lefimil, H. Osorio, R. Quatrini, D. Holmes, E. Jedlicki

Resultado de la investigación: Conference contribution

7 Citas (Scopus)

Resumen

Acidithiobacillus ferrooxidans is an acidophilic, chemolithotrophic bacterium that can gain energy and electrons by the oxidation of iron. PetI is an operon that encodes electron transport proteins involved in the reverse flow of electrons from iron to the NAD complex during iron oxidation. Although a substantial body of evidence describes the components and pathways for iron oxidation, little is known about their regulation. It is proposed that environmental iron concentrations and oxygen levels could influence the ability of A. ferrooxidans to oxidize iron as an energy source. We report initial investigators into the possibility that the expression of the PetI operon is regulated, inter alia, by the master transcription factor controlling iron uptake and homeostasis (Fur) and anaerobic metabolism (FNR). Potential DNA binding sites (boxes) for both regulators were predicted in the region of the PetI operon promoter and the binding of Fur to its cognate box was demonstrated experimentally. This represents the first attempt to unravel the mechanisms involved in the regulation of iron oxidation.

Idioma originalEnglish
Título de la publicación alojadaBiohydrometallurgy 2009
Páginas199-202
Número de páginas4
Volumen71-73
DOI
EstadoPublished - 2009
Evento18th International Biohydrometallurgy Symposium, IBS 2009 - Bariloche, Argentina
Duración: 13 sep 200917 sep 2009

Serie de la publicación

NombreAdvanced Materials Research
Volumen71-73
ISSN (versión impresa)10226680

Other

Other18th International Biohydrometallurgy Symposium, IBS 2009
PaísArgentina
CiudadBariloche
Período13/09/0917/09/09

Huella dactilar

Bacteria
Iron
Oxidation
Transcription factors
Electrons
Binding sites
Metabolism
DNA
Oxygen

ASJC Scopus subject areas

  • Engineering(all)

Citar esto

Lefimil, C., Osorio, H., Quatrini, R., Holmes, D., & Jedlicki, E. (2009). Regulation of expression of the PetI operon involved in iron oxidation in the biomining bacterium Acidithiobacillus ferrooxidans. En Biohydrometallurgy 2009 (Vol. 71-73, pp. 199-202). (Advanced Materials Research; Vol. 71-73). https://doi.org/10.4028/www.scientific.net/AMR.71-73.199
Lefimil, C. ; Osorio, H. ; Quatrini, R. ; Holmes, D. ; Jedlicki, E. / Regulation of expression of the PetI operon involved in iron oxidation in the biomining bacterium Acidithiobacillus ferrooxidans. Biohydrometallurgy 2009. Vol. 71-73 2009. pp. 199-202 (Advanced Materials Research).
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title = "Regulation of expression of the PetI operon involved in iron oxidation in the biomining bacterium Acidithiobacillus ferrooxidans",
abstract = "Acidithiobacillus ferrooxidans is an acidophilic, chemolithotrophic bacterium that can gain energy and electrons by the oxidation of iron. PetI is an operon that encodes electron transport proteins involved in the reverse flow of electrons from iron to the NAD complex during iron oxidation. Although a substantial body of evidence describes the components and pathways for iron oxidation, little is known about their regulation. It is proposed that environmental iron concentrations and oxygen levels could influence the ability of A. ferrooxidans to oxidize iron as an energy source. We report initial investigators into the possibility that the expression of the PetI operon is regulated, inter alia, by the master transcription factor controlling iron uptake and homeostasis (Fur) and anaerobic metabolism (FNR). Potential DNA binding sites (boxes) for both regulators were predicted in the region of the PetI operon promoter and the binding of Fur to its cognate box was demonstrated experimentally. This represents the first attempt to unravel the mechanisms involved in the regulation of iron oxidation.",
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Lefimil, C, Osorio, H, Quatrini, R, Holmes, D & Jedlicki, E 2009, Regulation of expression of the PetI operon involved in iron oxidation in the biomining bacterium Acidithiobacillus ferrooxidans. En Biohydrometallurgy 2009. vol. 71-73, Advanced Materials Research, vol. 71-73, pp. 199-202, 18th International Biohydrometallurgy Symposium, IBS 2009, Bariloche, Argentina, 13/09/09. https://doi.org/10.4028/www.scientific.net/AMR.71-73.199

Regulation of expression of the PetI operon involved in iron oxidation in the biomining bacterium Acidithiobacillus ferrooxidans. / Lefimil, C.; Osorio, H.; Quatrini, R.; Holmes, D.; Jedlicki, E.

Biohydrometallurgy 2009. Vol. 71-73 2009. p. 199-202 (Advanced Materials Research; Vol. 71-73).

Resultado de la investigación: Conference contribution

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AU - Osorio, H.

AU - Quatrini, R.

AU - Holmes, D.

AU - Jedlicki, E.

PY - 2009

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N2 - Acidithiobacillus ferrooxidans is an acidophilic, chemolithotrophic bacterium that can gain energy and electrons by the oxidation of iron. PetI is an operon that encodes electron transport proteins involved in the reverse flow of electrons from iron to the NAD complex during iron oxidation. Although a substantial body of evidence describes the components and pathways for iron oxidation, little is known about their regulation. It is proposed that environmental iron concentrations and oxygen levels could influence the ability of A. ferrooxidans to oxidize iron as an energy source. We report initial investigators into the possibility that the expression of the PetI operon is regulated, inter alia, by the master transcription factor controlling iron uptake and homeostasis (Fur) and anaerobic metabolism (FNR). Potential DNA binding sites (boxes) for both regulators were predicted in the region of the PetI operon promoter and the binding of Fur to its cognate box was demonstrated experimentally. This represents the first attempt to unravel the mechanisms involved in the regulation of iron oxidation.

AB - Acidithiobacillus ferrooxidans is an acidophilic, chemolithotrophic bacterium that can gain energy and electrons by the oxidation of iron. PetI is an operon that encodes electron transport proteins involved in the reverse flow of electrons from iron to the NAD complex during iron oxidation. Although a substantial body of evidence describes the components and pathways for iron oxidation, little is known about their regulation. It is proposed that environmental iron concentrations and oxygen levels could influence the ability of A. ferrooxidans to oxidize iron as an energy source. We report initial investigators into the possibility that the expression of the PetI operon is regulated, inter alia, by the master transcription factor controlling iron uptake and homeostasis (Fur) and anaerobic metabolism (FNR). Potential DNA binding sites (boxes) for both regulators were predicted in the region of the PetI operon promoter and the binding of Fur to its cognate box was demonstrated experimentally. This represents the first attempt to unravel the mechanisms involved in the regulation of iron oxidation.

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Lefimil C, Osorio H, Quatrini R, Holmes D, Jedlicki E. Regulation of expression of the PetI operon involved in iron oxidation in the biomining bacterium Acidithiobacillus ferrooxidans. En Biohydrometallurgy 2009. Vol. 71-73. 2009. p. 199-202. (Advanced Materials Research). https://doi.org/10.4028/www.scientific.net/AMR.71-73.199