Resumen
Acidithiobacillus ferrooxidans is an acidophilic, chemolithotrophic bacterium that can gain energy and electrons by the oxidation of iron. PetI is an operon that encodes electron transport proteins involved in the reverse flow of electrons from iron to the NAD complex during iron oxidation. Although a substantial body of evidence describes the components and pathways for iron oxidation, little is known about their regulation. It is proposed that environmental iron concentrations and oxygen levels could influence the ability of A. ferrooxidans to oxidize iron as an energy source. We report initial investigators into the possibility that the expression of the PetI operon is regulated, inter alia, by the master transcription factor controlling iron uptake and homeostasis (Fur) and anaerobic metabolism (FNR). Potential DNA binding sites (boxes) for both regulators were predicted in the region of the PetI operon promoter and the binding of Fur to its cognate box was demonstrated experimentally. This represents the first attempt to unravel the mechanisms involved in the regulation of iron oxidation.
| Idioma original | English |
|---|---|
| Título de la publicación alojada | Biohydrometallurgy 2009 |
| Páginas | 199-202 |
| Número de páginas | 4 |
| Volumen | 71-73 |
| DOI | |
| Estado | Published - 2009 |
| Evento | 18th International Biohydrometallurgy Symposium, IBS 2009 - Bariloche, Argentina Duración: 13 sep 2009 → 17 sep 2009 |
Serie de la publicación
| Nombre | Advanced Materials Research |
|---|---|
| Volumen | 71-73 |
| ISSN (versión impresa) | 10226680 |
Other
| Other | 18th International Biohydrometallurgy Symposium, IBS 2009 |
|---|---|
| País | Argentina |
| Ciudad | Bariloche |
| Período | 13/09/09 → 17/09/09 |
Huella dactilar
ASJC Scopus subject areas
- Engineering(all)
Citar esto
}
Regulation of expression of the PetI operon involved in iron oxidation in the biomining bacterium Acidithiobacillus ferrooxidans. / Lefimil, C.; Osorio, H.; Quatrini, R.; Holmes, D.; Jedlicki, E.
Biohydrometallurgy 2009. Vol. 71-73 2009. p. 199-202 (Advanced Materials Research; Vol. 71-73).Resultado de la investigación: Conference contribution
TY - GEN
T1 - Regulation of expression of the PetI operon involved in iron oxidation in the biomining bacterium Acidithiobacillus ferrooxidans
AU - Lefimil, C.
AU - Osorio, H.
AU - Quatrini, R.
AU - Holmes, D.
AU - Jedlicki, E.
PY - 2009
Y1 - 2009
N2 - Acidithiobacillus ferrooxidans is an acidophilic, chemolithotrophic bacterium that can gain energy and electrons by the oxidation of iron. PetI is an operon that encodes electron transport proteins involved in the reverse flow of electrons from iron to the NAD complex during iron oxidation. Although a substantial body of evidence describes the components and pathways for iron oxidation, little is known about their regulation. It is proposed that environmental iron concentrations and oxygen levels could influence the ability of A. ferrooxidans to oxidize iron as an energy source. We report initial investigators into the possibility that the expression of the PetI operon is regulated, inter alia, by the master transcription factor controlling iron uptake and homeostasis (Fur) and anaerobic metabolism (FNR). Potential DNA binding sites (boxes) for both regulators were predicted in the region of the PetI operon promoter and the binding of Fur to its cognate box was demonstrated experimentally. This represents the first attempt to unravel the mechanisms involved in the regulation of iron oxidation.
AB - Acidithiobacillus ferrooxidans is an acidophilic, chemolithotrophic bacterium that can gain energy and electrons by the oxidation of iron. PetI is an operon that encodes electron transport proteins involved in the reverse flow of electrons from iron to the NAD complex during iron oxidation. Although a substantial body of evidence describes the components and pathways for iron oxidation, little is known about their regulation. It is proposed that environmental iron concentrations and oxygen levels could influence the ability of A. ferrooxidans to oxidize iron as an energy source. We report initial investigators into the possibility that the expression of the PetI operon is regulated, inter alia, by the master transcription factor controlling iron uptake and homeostasis (Fur) and anaerobic metabolism (FNR). Potential DNA binding sites (boxes) for both regulators were predicted in the region of the PetI operon promoter and the binding of Fur to its cognate box was demonstrated experimentally. This represents the first attempt to unravel the mechanisms involved in the regulation of iron oxidation.
KW - Acidithiobacillus ferrooxidans
KW - FNR
KW - Fur
KW - Gene regulation
KW - Iron oxidation
KW - Peti
UR - http://www.scopus.com/inward/record.url?scp=72449165371&partnerID=8YFLogxK
U2 - 10.4028/www.scientific.net/AMR.71-73.199
DO - 10.4028/www.scientific.net/AMR.71-73.199
M3 - Conference contribution
AN - SCOPUS:72449165371
SN - 0878493220
SN - 9780878493227
VL - 71-73
T3 - Advanced Materials Research
SP - 199
EP - 202
BT - Biohydrometallurgy 2009
ER -