Regulation of biosynthesis of the basic fibroblast growth factor binding domains of heparan sulfate by heparan sulfate-N-deacetylase/N- sulfotransferase expression

M. Ishihara, Y. Guo, Z. Wei, Z. Yang, S. J. Swiedler, A. Orellana, C. B. Hirschberg

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

52 Citas (Scopus)

Resumen

Heparan sulfate-N-deacetylase/N-sulfotransferase catalyzes both the N- deacetylation and N-sulfation reactions that initiate the modification of the oligosaccharide backbone of heparan sulfate (HS). The glycosaminoglycan polymer appears to modulate the activity of growth factors by mediating their initial binding. To understand how the biosynthesis of these binding sites is regulated, a rat liver-derived cDNA encoding the above activities was overexpressed in a COS cell mutant (CM-15) that has reduced levels of the enzyme and binds poorly to immobilized basic fibroblast growth factor (bFGF). This resulted in increased synthesis of sulfated blocks of decasaccharide size or longer. These blocks exhibited high affinity binding to bFGF and contained a high content of 2-O-sulfated iduronate and at least five consecutive N-sulfated disaccharides. An increase in the synthesis of these high affinity blocks was not seen in transfected wild-type COS cells even though they showed a 4-fold increase of both enzyme activities, suggesting that once sufficient levels of highly sulfated blocks of saccharides with high affinity for bFGF are attained, no further synthesis of these domains occurs.

Idioma originalInglés
Páginas (desde-hasta)20091-20095
Número de páginas5
PublicaciónJournal of Biological Chemistry
Volumen268
N.º27
EstadoPublicada - 1993

Áreas temáticas de ASJC Scopus

  • Bioquímica
  • Biología molecular
  • Biología celular

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