Reduced invasion to human epithelial cell lines of Salmonella enterica serovar Typhi carrying S. Typhimurium sopD2

Annette N. Trombert, Paula I. Rodas, Guido C. Mora

Resultado de la investigación: Contribución a una revistaCarta

7 Citas (Scopus)

Resumen

Salmonella enterica serovar Typhi and Typhimurium are closely related serovars. However, S. Typhi, a human-specific pathogen, has 5% of genes as pseudogenes, far more than S. Typhimurium, which only has 1%. One of these pseudogenes corresponds to sopD2, which in S. Typhimurium encodes an effector protein involved in Salmonella-containing vacuole biogenesis in human epithelial cell lines, which is needed for full virulence of the pathogen. We investigated whether S. Typhi trans-complemented with the functional sopD2 gene from S. Typhimurium (sopD2 STM) would reduce the invasion of human epithelial cell lines. Our results showed that the presence of sopD2 STM in S. Typhi significantly modified the bacterial ability to alter cellular permeability and decrease the CFUs recovered after cell invasion of human epithelial cell line. These results add to mounting evidence that pseudogenes contribute to S. Typhi adaptation to humans.

Idioma originalInglés
Páginas (desde-hasta)150-156
Número de páginas7
PublicaciónFEMS Microbiology Letters
Volumen322
N.º2
DOI
EstadoPublicada - sep 2011

Áreas temáticas de ASJC Scopus

  • Microbiología
  • Genética
  • Biología molecular

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