Reduced expression of selected FASCICLIN-LIKE ARABINOGALACTAN PROTEIN genes associates with the abortion of kernels in field crops of Zea mays (maize) and of Arabidopsis seeds

Juan Ignacio Cagnola, Gonzalo Javier Dumont de Chassart, Silvia Elizabeth Ibarra, Claudio Chimenti, Martiniano María Ricardi, Brent Delzer, Hernán Ghiglione, Tong Zhu, María Elena Otegui, José Manuel Estevez, Jorge José Casal

Resultado de la investigación: Article

7 Citas (Scopus)

Resumen

Abortion of fertilized ovaries at the tip of the ear can generate significant yield losses in maize crops. To investigate the mechanisms involved in this process, 2 maize hybrids were grown in field crops at 2 sowing densities and under 3 irrigation regimes (well-watered control, drought before pollination, and drought during pollination), in all possible combinations. Samples of ear tips were taken 2–6 days after synchronous hand pollination and used for the analysis of gene expression and sugars. Glucose and fructose levels increased in kernels with high abortion risk. Several FASCICLIN-LIKE ARABINOGALACTAN PROTEIN (FLA) genes showed negative correlation with abortion. The expression of ZmFLA7 responded to drought only at the tip of the ear. The abundance of arabinogalactan protein (AGP) glycan epitopes decreased with drought and pharmacological treatments that reduce AGP activity enhanced the abortion of fertilized ovaries. Drought also reduced the expression of AthFLA9 in the siliques of Arabidopsis thaliana. Gain- and loss-of-function mutants of Arabidopsis showed a negative correlation between AthFLA9 and seed abortion. On the basis of gene expression patterns, pharmacological, and genetic evidence, we propose that stress-induced reductions in the expression of selected FLA genes enhance abortion of fertilized ovaries in maize and Arabidopsis.

Idioma originalEnglish
Páginas (desde-hasta)661-674
Número de páginas14
PublicaciónPlant Cell and Environment
Volumen41
N.º3
DOI
EstadoPublished - mar 2018

Huella dactilar

arabinogalactan proteins
abortion (plants)
Droughts
field crops
Arabidopsis
Zea mays
Seeds
Pollination
drought
corn
Ear
ears
Ovary
seeds
pollination
Genes
genes
Pharmacology
seed abortion
Gene Expression

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Citar esto

Cagnola, Juan Ignacio ; Dumont de Chassart, Gonzalo Javier ; Ibarra, Silvia Elizabeth ; Chimenti, Claudio ; Ricardi, Martiniano María ; Delzer, Brent ; Ghiglione, Hernán ; Zhu, Tong ; Otegui, María Elena ; Estevez, José Manuel ; Casal, Jorge José. / Reduced expression of selected FASCICLIN-LIKE ARABINOGALACTAN PROTEIN genes associates with the abortion of kernels in field crops of Zea mays (maize) and of Arabidopsis seeds. En: Plant Cell and Environment. 2018 ; Vol. 41, N.º 3. pp. 661-674.
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title = "Reduced expression of selected FASCICLIN-LIKE ARABINOGALACTAN PROTEIN genes associates with the abortion of kernels in field crops of Zea mays (maize) and of Arabidopsis seeds",
abstract = "Abortion of fertilized ovaries at the tip of the ear can generate significant yield losses in maize crops. To investigate the mechanisms involved in this process, 2 maize hybrids were grown in field crops at 2 sowing densities and under 3 irrigation regimes (well-watered control, drought before pollination, and drought during pollination), in all possible combinations. Samples of ear tips were taken 2–6 days after synchronous hand pollination and used for the analysis of gene expression and sugars. Glucose and fructose levels increased in kernels with high abortion risk. Several FASCICLIN-LIKE ARABINOGALACTAN PROTEIN (FLA) genes showed negative correlation with abortion. The expression of ZmFLA7 responded to drought only at the tip of the ear. The abundance of arabinogalactan protein (AGP) glycan epitopes decreased with drought and pharmacological treatments that reduce AGP activity enhanced the abortion of fertilized ovaries. Drought also reduced the expression of AthFLA9 in the siliques of Arabidopsis thaliana. Gain- and loss-of-function mutants of Arabidopsis showed a negative correlation between AthFLA9 and seed abortion. On the basis of gene expression patterns, pharmacological, and genetic evidence, we propose that stress-induced reductions in the expression of selected FLA genes enhance abortion of fertilized ovaries in maize and Arabidopsis.",
keywords = "corn, drought stress, FLA, seed abortion",
author = "Cagnola, {Juan Ignacio} and {Dumont de Chassart}, {Gonzalo Javier} and Ibarra, {Silvia Elizabeth} and Claudio Chimenti and Ricardi, {Martiniano Mar{\'i}a} and Brent Delzer and Hern{\'a}n Ghiglione and Tong Zhu and Otegui, {Mar{\'i}a Elena} and Estevez, {Jos{\'e} Manuel} and Casal, {Jorge Jos{\'e}}",
year = "2018",
month = "3",
doi = "10.1111/pce.13136",
language = "English",
volume = "41",
pages = "661--674",
journal = "Plant, Cell and Environment",
issn = "0140-7791",
publisher = "Wiley-Blackwell",
number = "3",

}

Cagnola, JI, Dumont de Chassart, GJ, Ibarra, SE, Chimenti, C, Ricardi, MM, Delzer, B, Ghiglione, H, Zhu, T, Otegui, ME, Estevez, JM & Casal, JJ 2018, 'Reduced expression of selected FASCICLIN-LIKE ARABINOGALACTAN PROTEIN genes associates with the abortion of kernels in field crops of Zea mays (maize) and of Arabidopsis seeds', Plant Cell and Environment, vol. 41, n.º 3, pp. 661-674. https://doi.org/10.1111/pce.13136

Reduced expression of selected FASCICLIN-LIKE ARABINOGALACTAN PROTEIN genes associates with the abortion of kernels in field crops of Zea mays (maize) and of Arabidopsis seeds. / Cagnola, Juan Ignacio; Dumont de Chassart, Gonzalo Javier; Ibarra, Silvia Elizabeth; Chimenti, Claudio; Ricardi, Martiniano María; Delzer, Brent; Ghiglione, Hernán; Zhu, Tong; Otegui, María Elena; Estevez, José Manuel; Casal, Jorge José.

En: Plant Cell and Environment, Vol. 41, N.º 3, 03.2018, p. 661-674.

Resultado de la investigación: Article

TY - JOUR

T1 - Reduced expression of selected FASCICLIN-LIKE ARABINOGALACTAN PROTEIN genes associates with the abortion of kernels in field crops of Zea mays (maize) and of Arabidopsis seeds

AU - Cagnola, Juan Ignacio

AU - Dumont de Chassart, Gonzalo Javier

AU - Ibarra, Silvia Elizabeth

AU - Chimenti, Claudio

AU - Ricardi, Martiniano María

AU - Delzer, Brent

AU - Ghiglione, Hernán

AU - Zhu, Tong

AU - Otegui, María Elena

AU - Estevez, José Manuel

AU - Casal, Jorge José

PY - 2018/3

Y1 - 2018/3

N2 - Abortion of fertilized ovaries at the tip of the ear can generate significant yield losses in maize crops. To investigate the mechanisms involved in this process, 2 maize hybrids were grown in field crops at 2 sowing densities and under 3 irrigation regimes (well-watered control, drought before pollination, and drought during pollination), in all possible combinations. Samples of ear tips were taken 2–6 days after synchronous hand pollination and used for the analysis of gene expression and sugars. Glucose and fructose levels increased in kernels with high abortion risk. Several FASCICLIN-LIKE ARABINOGALACTAN PROTEIN (FLA) genes showed negative correlation with abortion. The expression of ZmFLA7 responded to drought only at the tip of the ear. The abundance of arabinogalactan protein (AGP) glycan epitopes decreased with drought and pharmacological treatments that reduce AGP activity enhanced the abortion of fertilized ovaries. Drought also reduced the expression of AthFLA9 in the siliques of Arabidopsis thaliana. Gain- and loss-of-function mutants of Arabidopsis showed a negative correlation between AthFLA9 and seed abortion. On the basis of gene expression patterns, pharmacological, and genetic evidence, we propose that stress-induced reductions in the expression of selected FLA genes enhance abortion of fertilized ovaries in maize and Arabidopsis.

AB - Abortion of fertilized ovaries at the tip of the ear can generate significant yield losses in maize crops. To investigate the mechanisms involved in this process, 2 maize hybrids were grown in field crops at 2 sowing densities and under 3 irrigation regimes (well-watered control, drought before pollination, and drought during pollination), in all possible combinations. Samples of ear tips were taken 2–6 days after synchronous hand pollination and used for the analysis of gene expression and sugars. Glucose and fructose levels increased in kernels with high abortion risk. Several FASCICLIN-LIKE ARABINOGALACTAN PROTEIN (FLA) genes showed negative correlation with abortion. The expression of ZmFLA7 responded to drought only at the tip of the ear. The abundance of arabinogalactan protein (AGP) glycan epitopes decreased with drought and pharmacological treatments that reduce AGP activity enhanced the abortion of fertilized ovaries. Drought also reduced the expression of AthFLA9 in the siliques of Arabidopsis thaliana. Gain- and loss-of-function mutants of Arabidopsis showed a negative correlation between AthFLA9 and seed abortion. On the basis of gene expression patterns, pharmacological, and genetic evidence, we propose that stress-induced reductions in the expression of selected FLA genes enhance abortion of fertilized ovaries in maize and Arabidopsis.

KW - corn

KW - drought stress

KW - FLA

KW - seed abortion

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U2 - 10.1111/pce.13136

DO - 10.1111/pce.13136

M3 - Article

C2 - 29314044

AN - SCOPUS:85041231978

VL - 41

SP - 661

EP - 674

JO - Plant, Cell and Environment

JF - Plant, Cell and Environment

SN - 0140-7791

IS - 3

ER -