Resumen
The architectural organization of the genome and regulatory proteins within the nucleus supports gene expression in a physiologically regulated manner. In osteoblastic cells ligand activation induces a nuclear punctate distribution of the 1α,25-dihydroxy vitamin D3 (1α,25(OH)2D3) receptor (VDR) and promotes its interaction with transcriptional coactivators such as SRC-1, NCoA-62/Skip, and DRIP205. Here, we discuss evidence demonstrating that in osteoblastic cells VDR binds to the nuclear matrix fraction in a 1α,25(OH)2D3-dependent manner. This interaction occurs rapidly after exposure to 1α,25(OH)2D3 and does not require a functional VDR DNA binding domain. The nuclear matrix-bound VDR molecules colocalize with the also nuclear matrix-associated coactivator DRIP205. We propose a model where the rapid association of VDR with the nuclear matrix fraction represents an event that follows 1α,25(OH)2D3-dependent nuclear localization of VDR, but that precedes 1α,25(OH)2D3-dependent transcriptional upregulation at target genes.
Idioma original | Inglés |
---|---|
Páginas (desde-hasta) | 156-158 |
Número de páginas | 3 |
Publicación | Journal of Steroid Biochemistry and Molecular Biology |
Volumen | 121 |
N.º | 1-2 |
DOI | |
Estado | Publicada - jul. 2010 |
Áreas temáticas de ASJC Scopus
- Endocrinología, diabetes y metabolismo
- Bioquímica
- Medicina molecular
- Biología molecular
- Endocrinología
- Bioquímica clínica
- Biología celular