Recovering Streptococcus mutans over restorations by the tray technique - A randomized clinical study

Patricio Vildósola Grez, Patricia Palma Fluxa, Paula Zuñiga Saavedra, Eduardo Fernandez Godoy, Osmir Batista de Oliveira Junior, Gustavo Moncada Cortés

Resultado de la investigación: Article

2 Citas (Scopus)

Resumen

Aim: To correlate the tray technique for isolation and counting of Streptococcus mutans over dental restoration with the toothpick technique. Methods: Forty 18-50-year-old patients of both genders with high cariogenic risk were selected. Inclusion criteria were: occlusal restoration evaluated as alpha, according to the Ryge's criteria (examiner' Cohen-Kappa 0.8) and 2 years old maximum age. Each patient provided a saliva sample (control group S) and two samples of dental biofilm from amalgam or resin-based composite restorations in the same patient, one obtained with the toothpick technique (group TT), and the other from homologous tooth restoration with the tray technique (group TR). This method involves only a direct impression on the occlusal surfaces by a section of a fluoride tray with trypticase yeast extract cysteine sucrose with bacitracin (TYCSB) agar previously prepared and solidified. The samples were incubated at 37 °C for 48 h and the colony-forming units (CFU) were counted. The results were statistically analyzed with SPSS software using the linear regression method at 95% confidence level. Results: TR was able to isolate S. mutans, and there was a significant positive correlation (r=0.95) with TT. There was no significant correlation between TR and control group S (r=0.47). Conclusions: TR was able to isolate and count S. mutans from the dental biofilm of dental restorations with a significant correlation with the TT group.

Idioma originalEnglish
Páginas (desde-hasta)292-297
Número de páginas6
PublicaciónBrazilian Journal of Oral Sciences
Volumen12
N.º4
EstadoPublished - 1 oct 2013

Huella dactilar

Streptococcus mutans
Tooth
Biofilms
Synthetic Resins
Dental Amalgam
Bacitracin
Control Groups
Fluorides
Saliva
Agar
Cysteine
Sucrose
Linear Models
Stem Cells
Software
Yeasts
Clinical Studies

ASJC Scopus subject areas

  • Dentistry(all)

Citar esto

Grez, P. V., Fluxa, P. P., Saavedra, P. Z., Godoy, E. F., de Oliveira Junior, O. B., & Cortés, G. M. (2013). Recovering Streptococcus mutans over restorations by the tray technique - A randomized clinical study. Brazilian Journal of Oral Sciences, 12(4), 292-297.
Grez, Patricio Vildósola ; Fluxa, Patricia Palma ; Saavedra, Paula Zuñiga ; Godoy, Eduardo Fernandez ; de Oliveira Junior, Osmir Batista ; Cortés, Gustavo Moncada. / Recovering Streptococcus mutans over restorations by the tray technique - A randomized clinical study. En: Brazilian Journal of Oral Sciences. 2013 ; Vol. 12, N.º 4. pp. 292-297.
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abstract = "Aim: To correlate the tray technique for isolation and counting of Streptococcus mutans over dental restoration with the toothpick technique. Methods: Forty 18-50-year-old patients of both genders with high cariogenic risk were selected. Inclusion criteria were: occlusal restoration evaluated as alpha, according to the Ryge's criteria (examiner' Cohen-Kappa 0.8) and 2 years old maximum age. Each patient provided a saliva sample (control group S) and two samples of dental biofilm from amalgam or resin-based composite restorations in the same patient, one obtained with the toothpick technique (group TT), and the other from homologous tooth restoration with the tray technique (group TR). This method involves only a direct impression on the occlusal surfaces by a section of a fluoride tray with trypticase yeast extract cysteine sucrose with bacitracin (TYCSB) agar previously prepared and solidified. The samples were incubated at 37 °C for 48 h and the colony-forming units (CFU) were counted. The results were statistically analyzed with SPSS software using the linear regression method at 95{\%} confidence level. Results: TR was able to isolate S. mutans, and there was a significant positive correlation (r=0.95) with TT. There was no significant correlation between TR and control group S (r=0.47). Conclusions: TR was able to isolate and count S. mutans from the dental biofilm of dental restorations with a significant correlation with the TT group.",
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Grez, PV, Fluxa, PP, Saavedra, PZ, Godoy, EF, de Oliveira Junior, OB & Cortés, GM 2013, 'Recovering Streptococcus mutans over restorations by the tray technique - A randomized clinical study', Brazilian Journal of Oral Sciences, vol. 12, n.º 4, pp. 292-297.

Recovering Streptococcus mutans over restorations by the tray technique - A randomized clinical study. / Grez, Patricio Vildósola; Fluxa, Patricia Palma; Saavedra, Paula Zuñiga; Godoy, Eduardo Fernandez; de Oliveira Junior, Osmir Batista; Cortés, Gustavo Moncada.

En: Brazilian Journal of Oral Sciences, Vol. 12, N.º 4, 01.10.2013, p. 292-297.

Resultado de la investigación: Article

TY - JOUR

T1 - Recovering Streptococcus mutans over restorations by the tray technique - A randomized clinical study

AU - Grez, Patricio Vildósola

AU - Fluxa, Patricia Palma

AU - Saavedra, Paula Zuñiga

AU - Godoy, Eduardo Fernandez

AU - de Oliveira Junior, Osmir Batista

AU - Cortés, Gustavo Moncada

PY - 2013/10/1

Y1 - 2013/10/1

N2 - Aim: To correlate the tray technique for isolation and counting of Streptococcus mutans over dental restoration with the toothpick technique. Methods: Forty 18-50-year-old patients of both genders with high cariogenic risk were selected. Inclusion criteria were: occlusal restoration evaluated as alpha, according to the Ryge's criteria (examiner' Cohen-Kappa 0.8) and 2 years old maximum age. Each patient provided a saliva sample (control group S) and two samples of dental biofilm from amalgam or resin-based composite restorations in the same patient, one obtained with the toothpick technique (group TT), and the other from homologous tooth restoration with the tray technique (group TR). This method involves only a direct impression on the occlusal surfaces by a section of a fluoride tray with trypticase yeast extract cysteine sucrose with bacitracin (TYCSB) agar previously prepared and solidified. The samples were incubated at 37 °C for 48 h and the colony-forming units (CFU) were counted. The results were statistically analyzed with SPSS software using the linear regression method at 95% confidence level. Results: TR was able to isolate S. mutans, and there was a significant positive correlation (r=0.95) with TT. There was no significant correlation between TR and control group S (r=0.47). Conclusions: TR was able to isolate and count S. mutans from the dental biofilm of dental restorations with a significant correlation with the TT group.

AB - Aim: To correlate the tray technique for isolation and counting of Streptococcus mutans over dental restoration with the toothpick technique. Methods: Forty 18-50-year-old patients of both genders with high cariogenic risk were selected. Inclusion criteria were: occlusal restoration evaluated as alpha, according to the Ryge's criteria (examiner' Cohen-Kappa 0.8) and 2 years old maximum age. Each patient provided a saliva sample (control group S) and two samples of dental biofilm from amalgam or resin-based composite restorations in the same patient, one obtained with the toothpick technique (group TT), and the other from homologous tooth restoration with the tray technique (group TR). This method involves only a direct impression on the occlusal surfaces by a section of a fluoride tray with trypticase yeast extract cysteine sucrose with bacitracin (TYCSB) agar previously prepared and solidified. The samples were incubated at 37 °C for 48 h and the colony-forming units (CFU) were counted. The results were statistically analyzed with SPSS software using the linear regression method at 95% confidence level. Results: TR was able to isolate S. mutans, and there was a significant positive correlation (r=0.95) with TT. There was no significant correlation between TR and control group S (r=0.47). Conclusions: TR was able to isolate and count S. mutans from the dental biofilm of dental restorations with a significant correlation with the TT group.

KW - Dental caries

KW - Dental restoration

KW - Streptococcus mutans

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M3 - Article

AN - SCOPUS:84892994623

VL - 12

SP - 292

EP - 297

JO - Brazilian Journal of Oral Sciences

JF - Brazilian Journal of Oral Sciences

SN - 1677-3225

IS - 4

ER -

Grez PV, Fluxa PP, Saavedra PZ, Godoy EF, de Oliveira Junior OB, Cortés GM. Recovering Streptococcus mutans over restorations by the tray technique - A randomized clinical study. Brazilian Journal of Oral Sciences. 2013 oct 1;12(4):292-297.