Reconstitution of recombinant chromatin establishes a requirement for histone-tail modifications during chromatin assembly and transcription

Alejandra Loyola, Gary LeRoy, Yuh Hwa Wang, Danny Reinberg

Resultado de la investigación: Article

94 Citas (Scopus)

Resumen

The human ISWI-containing factor RSF (remodeling and spacing factor) was found to mediate nucleosome deposition and, in the presence of ATP, generate regularly spaced nucleosome arrays. Using this system, recombinant chromatin was reconstituted with bacterially produced histones. Acetylation of the histone tails was found to play an important role in establishing regularly spaced nucleosome arrays. Recombinant chromatin lacking histone acetylation was impaired in directing transcription. Histone-tail modifications were found to regulate transcription from the recombinant chromatin. Acetylation of the histone tails by p300 was found to increase transcription. Methylation of the histone H3 tail by Suv39H1 was found to repress transcription in an HP1-dependent manner. The effects of histone-tail modifications were observed in nuclear extracts. A highly reconstituted RNA polymerase II transcription system was refractory to the effect imposed by acetylation and methylation.

Idioma originalEnglish
Páginas (desde-hasta)2837-2851
Número de páginas15
PublicaciónGenes and Development
Volumen15
N.º21
EstadoPublished - 1 nov 2001

Huella dactilar

Histone Code
Chromatin Assembly and Disassembly
Histones
Chromatin
Tail
Acetylation
Nucleosomes
Methylation
RNA Polymerase II
Adenosine Triphosphate

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Citar esto

Loyola, Alejandra ; LeRoy, Gary ; Wang, Yuh Hwa ; Reinberg, Danny. / Reconstitution of recombinant chromatin establishes a requirement for histone-tail modifications during chromatin assembly and transcription. En: Genes and Development. 2001 ; Vol. 15, N.º 21. pp. 2837-2851.
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Reconstitution of recombinant chromatin establishes a requirement for histone-tail modifications during chromatin assembly and transcription. / Loyola, Alejandra; LeRoy, Gary; Wang, Yuh Hwa; Reinberg, Danny.

En: Genes and Development, Vol. 15, N.º 21, 01.11.2001, p. 2837-2851.

Resultado de la investigación: Article

TY - JOUR

T1 - Reconstitution of recombinant chromatin establishes a requirement for histone-tail modifications during chromatin assembly and transcription

AU - Loyola, Alejandra

AU - LeRoy, Gary

AU - Wang, Yuh Hwa

AU - Reinberg, Danny

PY - 2001/11/1

Y1 - 2001/11/1

N2 - The human ISWI-containing factor RSF (remodeling and spacing factor) was found to mediate nucleosome deposition and, in the presence of ATP, generate regularly spaced nucleosome arrays. Using this system, recombinant chromatin was reconstituted with bacterially produced histones. Acetylation of the histone tails was found to play an important role in establishing regularly spaced nucleosome arrays. Recombinant chromatin lacking histone acetylation was impaired in directing transcription. Histone-tail modifications were found to regulate transcription from the recombinant chromatin. Acetylation of the histone tails by p300 was found to increase transcription. Methylation of the histone H3 tail by Suv39H1 was found to repress transcription in an HP1-dependent manner. The effects of histone-tail modifications were observed in nuclear extracts. A highly reconstituted RNA polymerase II transcription system was refractory to the effect imposed by acetylation and methylation.

AB - The human ISWI-containing factor RSF (remodeling and spacing factor) was found to mediate nucleosome deposition and, in the presence of ATP, generate regularly spaced nucleosome arrays. Using this system, recombinant chromatin was reconstituted with bacterially produced histones. Acetylation of the histone tails was found to play an important role in establishing regularly spaced nucleosome arrays. Recombinant chromatin lacking histone acetylation was impaired in directing transcription. Histone-tail modifications were found to regulate transcription from the recombinant chromatin. Acetylation of the histone tails by p300 was found to increase transcription. Methylation of the histone H3 tail by Suv39H1 was found to repress transcription in an HP1-dependent manner. The effects of histone-tail modifications were observed in nuclear extracts. A highly reconstituted RNA polymerase II transcription system was refractory to the effect imposed by acetylation and methylation.

KW - Acetylation

KW - Chromatin assembly

KW - Chromatin transcription

KW - Histones tails

KW - Methylation

KW - RSF

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M3 - Article

C2 - 11691835

AN - SCOPUS:0035499905

VL - 15

SP - 2837

EP - 2851

JO - Genes and Development

JF - Genes and Development

SN - 0890-9369

IS - 21

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