Rapid purification of bacterial plasmids and coliphage M13 RF without CsCl centrifugation

D. S. Holmes

Resultado de la investigación: Article

7 Citas (Scopus)

Resumen

A simple and rapid procedure for the purification of plasmids from Escherichia coli K12 has been developed. Bacterial cells are subjected to the boiling procedure [D. S. Holmes, and M. Quigley Anal. Biochem. 114, 193-197 (1981)] followed by removal of contaminating RNA by chromatography on Sepharose 2B and of genomic DNA by acid-phenol extraction. Plasmids are recovered with good yield. They can be restricted and ligated and will transform host cells. A simple modification of the procedure allows it to be used for the isolation of coliphage M13 RF DNA.

Idioma originalEnglish
Páginas (desde-hasta)428-433
Número de páginas6
PublicaciónAnalytical Biochemistry
Volumen127
N.º2
DOI
EstadoPublished - 1 ene 1982

Huella dactilar

Bacteriophage M13
Centrifugation
Purification
Plasmids
DNA
Chromatography
Phenol
Sepharose
Boiling liquids
Escherichia coli
Escherichia coli K12
Agarose Chromatography
RNA
Acids
cesium chloride

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Citar esto

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Rapid purification of bacterial plasmids and coliphage M13 RF without CsCl centrifugation. / Holmes, D. S.

En: Analytical Biochemistry, Vol. 127, N.º 2, 01.01.1982, p. 428-433.

Resultado de la investigación: Article

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