Rapid purification of bacterial plasmids and coliphage M13 RF without CsCl centrifugation

D. S. Holmes

Resultado de la investigación: Contribución a una revistaArtículo

7 Citas (Scopus)

Resumen

A simple and rapid procedure for the purification of plasmids from Escherichia coli K12 has been developed. Bacterial cells are subjected to the boiling procedure [D. S. Holmes, and M. Quigley Anal. Biochem. 114, 193-197 (1981)] followed by removal of contaminating RNA by chromatography on Sepharose 2B and of genomic DNA by acid-phenol extraction. Plasmids are recovered with good yield. They can be restricted and ligated and will transform host cells. A simple modification of the procedure allows it to be used for the isolation of coliphage M13 RF DNA.

Idioma originalInglés
Páginas (desde-hasta)428-433
Número de páginas6
PublicaciónAnalytical Biochemistry
Volumen127
N.º2
DOI
EstadoPublicada - 1 ene 1982

Áreas temáticas de ASJC Scopus

  • Biofísica
  • Bioquímica
  • Biología molecular
  • Biología celular

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