Rab35 functions in axon elongation are regulated by P53-related protein kinase in a mechanism that involves Rab35 protein degradation and the microtubule-associated protein 1B

David Villarroel-Campos, Daniel R. Henríquez, Felipe J. Bodaleo, Mai E. Oguchi, Francisca C. Bronfman, Mitsunori Fukuda, Christian Gonzalez-Billault

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

34 Citas (Scopus)


Rab35 is a key protein for cargo loading in the recycling endosome. In neuronal immortalized cells, Rab35 promotes neurite differentiation. Here we describe that Rab35 favors axon elongation in rat primary neurons in an activity-dependent manner. In addition, we show that the p53-related protein kinase (PRPK) negatively regulates axonal elongation by reducing Rab35 protein levels through the ubiquitin-proteasome degradation pathway. PRPK-induced Rab35 degradation is regulated by its interaction with microtubuleassociated protein 1B (MAP1B), a microtubule stabilizing binding protein essential for axon elongation. Consistently, axon defects found in MAP1B knock-out neurons were reversed by Rab35 overexpression or PRPK inactivation suggesting an epistatic relationship among these proteins. These results define a novel mechanism to support axonal elongation, by which MAP1B prevents PRPK-induced Rab35 degradation. Such a mechanism allows Rab35-mediated axonal elongation and connects the regulation of actin dynamics withmembrane trafficking. In addition, our study reveals for the first time that the ubiquitin-proteasome degradation pathway regulates a Rab GTPase.

Idioma originalInglés
Páginas (desde-hasta)7298-7313
Número de páginas16
PublicaciónJournal of Neuroscience
EstadoPublicada - 6 jul. 2016
Publicado de forma externa

Áreas temáticas de ASJC Scopus

  • Neurociencias General

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