Quantum dot-based assay for Cu2+ quantification in bacterial cell culture

V. Durán-Toro, A. Gran-Scheuch, N. Órdenes-Aenishanslins, J. P. Monrás, L. A. Saona, F. A. Venegas, T. G. Chasteen, D. Bravo, J. M. Pérez-Donoso

Resultado de la investigación: Article

14 Citas (Scopus)

Resumen

A simple and sensitive method for quantification of nanomolar copper with a detection limit of 1.2 × 10-10 M and a linear range from 10-9 to 10-8 M is reported. For the most useful analytical concentration of quantum dots, 1160 μg/ml, a 1/Ksv value of 11 μM Cu2+ was determined. The method is based on the interaction of Cu2+ with glutathione-capped CdTe quantum dots (CdTe-GSH QDs) synthesized by a simple and economic biomimetic method. Green CdTe-GSH QDs displayed the best performance in copper quantification when QDs of different sizes/colors were tested. Cu2+ quantification is highly selective given that no significant interference of QDs with 19 ions was observed. No significant effects on Cu2+ quantification were determined when different reaction matrices such as distilled water, tap water, and different bacterial growth media were tested. The method was used to determine copper uptake kinetics on Escherichia coli cultures. QD-based quantification of copper on bacterial supernatants was compared with atomic absorption spectroscopy as a means of confirming the accuracy of the reported method. The mechanism of Cu2+-mediated QD fluorescence quenching was associated with nanoparticle decomposition.

Idioma originalEnglish
Páginas (desde-hasta)30-36
Número de páginas7
PublicaciónAnalytical Biochemistry
Volumen450
N.º1
DOI
EstadoPublished - 1 abr 2014

Huella dactilar

Quantum Dots
Cell culture
Semiconductor quantum dots
Copper
Assays
Cell Culture Techniques
Atomic spectroscopy
Water
Biomimetics
Absorption spectroscopy
Escherichia coli
Glutathione
Quenching
Fluorescence
Nanoparticles
Ions
Limit of Detection
Color
Spectrum Analysis
Decomposition

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Citar esto

Durán-Toro, V., Gran-Scheuch, A., Órdenes-Aenishanslins, N., Monrás, J. P., Saona, L. A., Venegas, F. A., ... Pérez-Donoso, J. M. (2014). Quantum dot-based assay for Cu2+ quantification in bacterial cell culture. Analytical Biochemistry, 450(1), 30-36. https://doi.org/10.1016/j.ab.2014.01.001
Durán-Toro, V. ; Gran-Scheuch, A. ; Órdenes-Aenishanslins, N. ; Monrás, J. P. ; Saona, L. A. ; Venegas, F. A. ; Chasteen, T. G. ; Bravo, D. ; Pérez-Donoso, J. M. / Quantum dot-based assay for Cu2+ quantification in bacterial cell culture. En: Analytical Biochemistry. 2014 ; Vol. 450, N.º 1. pp. 30-36.
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abstract = "A simple and sensitive method for quantification of nanomolar copper with a detection limit of 1.2 × 10-10 M and a linear range from 10-9 to 10-8 M is reported. For the most useful analytical concentration of quantum dots, 1160 μg/ml, a 1/Ksv value of 11 μM Cu2+ was determined. The method is based on the interaction of Cu2+ with glutathione-capped CdTe quantum dots (CdTe-GSH QDs) synthesized by a simple and economic biomimetic method. Green CdTe-GSH QDs displayed the best performance in copper quantification when QDs of different sizes/colors were tested. Cu2+ quantification is highly selective given that no significant interference of QDs with 19 ions was observed. No significant effects on Cu2+ quantification were determined when different reaction matrices such as distilled water, tap water, and different bacterial growth media were tested. The method was used to determine copper uptake kinetics on Escherichia coli cultures. QD-based quantification of copper on bacterial supernatants was compared with atomic absorption spectroscopy as a means of confirming the accuracy of the reported method. The mechanism of Cu2+-mediated QD fluorescence quenching was associated with nanoparticle decomposition.",
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Durán-Toro, V, Gran-Scheuch, A, Órdenes-Aenishanslins, N, Monrás, JP, Saona, LA, Venegas, FA, Chasteen, TG, Bravo, D & Pérez-Donoso, JM 2014, 'Quantum dot-based assay for Cu2+ quantification in bacterial cell culture', Analytical Biochemistry, vol. 450, n.º 1, pp. 30-36. https://doi.org/10.1016/j.ab.2014.01.001

Quantum dot-based assay for Cu2+ quantification in bacterial cell culture. / Durán-Toro, V.; Gran-Scheuch, A.; Órdenes-Aenishanslins, N.; Monrás, J. P.; Saona, L. A.; Venegas, F. A.; Chasteen, T. G.; Bravo, D.; Pérez-Donoso, J. M.

En: Analytical Biochemistry, Vol. 450, N.º 1, 01.04.2014, p. 30-36.

Resultado de la investigación: Article

TY - JOUR

T1 - Quantum dot-based assay for Cu2+ quantification in bacterial cell culture

AU - Durán-Toro, V.

AU - Gran-Scheuch, A.

AU - Órdenes-Aenishanslins, N.

AU - Monrás, J. P.

AU - Saona, L. A.

AU - Venegas, F. A.

AU - Chasteen, T. G.

AU - Bravo, D.

AU - Pérez-Donoso, J. M.

PY - 2014/4/1

Y1 - 2014/4/1

N2 - A simple and sensitive method for quantification of nanomolar copper with a detection limit of 1.2 × 10-10 M and a linear range from 10-9 to 10-8 M is reported. For the most useful analytical concentration of quantum dots, 1160 μg/ml, a 1/Ksv value of 11 μM Cu2+ was determined. The method is based on the interaction of Cu2+ with glutathione-capped CdTe quantum dots (CdTe-GSH QDs) synthesized by a simple and economic biomimetic method. Green CdTe-GSH QDs displayed the best performance in copper quantification when QDs of different sizes/colors were tested. Cu2+ quantification is highly selective given that no significant interference of QDs with 19 ions was observed. No significant effects on Cu2+ quantification were determined when different reaction matrices such as distilled water, tap water, and different bacterial growth media were tested. The method was used to determine copper uptake kinetics on Escherichia coli cultures. QD-based quantification of copper on bacterial supernatants was compared with atomic absorption spectroscopy as a means of confirming the accuracy of the reported method. The mechanism of Cu2+-mediated QD fluorescence quenching was associated with nanoparticle decomposition.

AB - A simple and sensitive method for quantification of nanomolar copper with a detection limit of 1.2 × 10-10 M and a linear range from 10-9 to 10-8 M is reported. For the most useful analytical concentration of quantum dots, 1160 μg/ml, a 1/Ksv value of 11 μM Cu2+ was determined. The method is based on the interaction of Cu2+ with glutathione-capped CdTe quantum dots (CdTe-GSH QDs) synthesized by a simple and economic biomimetic method. Green CdTe-GSH QDs displayed the best performance in copper quantification when QDs of different sizes/colors were tested. Cu2+ quantification is highly selective given that no significant interference of QDs with 19 ions was observed. No significant effects on Cu2+ quantification were determined when different reaction matrices such as distilled water, tap water, and different bacterial growth media were tested. The method was used to determine copper uptake kinetics on Escherichia coli cultures. QD-based quantification of copper on bacterial supernatants was compared with atomic absorption spectroscopy as a means of confirming the accuracy of the reported method. The mechanism of Cu2+-mediated QD fluorescence quenching was associated with nanoparticle decomposition.

KW - Bacterial copper uptake kinetics

KW - Biomimetic synthesis

KW - CdTe-GSH

KW - Copper quantification

KW - Quantum dots

KW - Stern-Volmer

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U2 - 10.1016/j.ab.2014.01.001

DO - 10.1016/j.ab.2014.01.001

M3 - Article

C2 - 24433980

AN - SCOPUS:84893553241

VL - 450

SP - 30

EP - 36

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 1

ER -

Durán-Toro V, Gran-Scheuch A, Órdenes-Aenishanslins N, Monrás JP, Saona LA, Venegas FA y otros. Quantum dot-based assay for Cu2+ quantification in bacterial cell culture. Analytical Biochemistry. 2014 abr 1;450(1):30-36. https://doi.org/10.1016/j.ab.2014.01.001