TY - JOUR
T1 - Pseudomonas violetae sp. nov. and Pseudomonas emilianonis sp. nov., two new species with the ability to degrade TNT isolated from soil samples at Deception Island, maritime Antarctica
AU - Carrasco, Valentina
AU - Roldán, Diego M.
AU - Valenzuela-Ibaceta, Felipe
AU - Lagos-Moraga, Sebastián
AU - Dietz-Vargas, Claudio
AU - Menes, Rodolfo Javier
AU - Pérez-Donoso, José M.
N1 - Publisher Copyright:
© 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2024/1
Y1 - 2024/1
N2 - Two motile, rod-shaped, Gram-stain-negative bacterial strains, TNT11T and TNT19T, were isolated from soil samples collected at Deception Island, Antarctica. According to the 16S rRNA gene sequence similarity, both strains belong to the genus Pseudomonas. Further genomic analyses based on ANI and dDDH suggested that these strains were new species. Growth of strain TNT11T is observed at 0–30 ℃ (optimum, 20 ℃), pH 4.0–9.0 (optimum, pH 6.0) and in the presence of 0–5.0% NaCl (optimum, 1% NaCl), while for TNT19T is observed at 0–30 ℃ (optimum between 15 and 20 ℃), pH 5.0–9.0 (optimum, pH 6.0) and in the presence of 0–5.0% NaCl (optimum between 0 and 1% NaCl). The fatty acid profile consists of the major compounds; C16:0 and C16:1 ω6 for TNT11T, and C16:0 and C12:0 for TNT19T. Based on the draft genome sequences, the DNA G + C content for TNT11T is 60.43 mol% and 58.60 mol% for TNT19T. Based on this polyphasic study, TNT11T and TNT19T represent two novel species of the genus Pseudomonas, for which the proposed names are Pseudomonas violetae sp. nov. and Pseudomonas emilianonis sp. nov., respectively. The type strains are Pseudomonas violetae TNT11T (= RGM 3443T = LMG 32959T) and Pseudomonas emilianonis TNT19T (= RGM 3442T = LMG 32960T). Strains TNT11T and TNT19T were deposited to CChRGM and BCCM/LMG with entry numbers RGM 3443/LMG 32959 and RGM 3442/LMG 32960, respectively.
AB - Two motile, rod-shaped, Gram-stain-negative bacterial strains, TNT11T and TNT19T, were isolated from soil samples collected at Deception Island, Antarctica. According to the 16S rRNA gene sequence similarity, both strains belong to the genus Pseudomonas. Further genomic analyses based on ANI and dDDH suggested that these strains were new species. Growth of strain TNT11T is observed at 0–30 ℃ (optimum, 20 ℃), pH 4.0–9.0 (optimum, pH 6.0) and in the presence of 0–5.0% NaCl (optimum, 1% NaCl), while for TNT19T is observed at 0–30 ℃ (optimum between 15 and 20 ℃), pH 5.0–9.0 (optimum, pH 6.0) and in the presence of 0–5.0% NaCl (optimum between 0 and 1% NaCl). The fatty acid profile consists of the major compounds; C16:0 and C16:1 ω6 for TNT11T, and C16:0 and C12:0 for TNT19T. Based on the draft genome sequences, the DNA G + C content for TNT11T is 60.43 mol% and 58.60 mol% for TNT19T. Based on this polyphasic study, TNT11T and TNT19T represent two novel species of the genus Pseudomonas, for which the proposed names are Pseudomonas violetae sp. nov. and Pseudomonas emilianonis sp. nov., respectively. The type strains are Pseudomonas violetae TNT11T (= RGM 3443T = LMG 32959T) and Pseudomonas emilianonis TNT19T (= RGM 3442T = LMG 32960T). Strains TNT11T and TNT19T were deposited to CChRGM and BCCM/LMG with entry numbers RGM 3443/LMG 32959 and RGM 3442/LMG 32960, respectively.
KW - Pseudomonadaceae
KW - Pseudomonas sp
KW - TNT degradation, bioremediation, antarctica
UR - http://www.scopus.com/inward/record.url?scp=85180494572&partnerID=8YFLogxK
U2 - 10.1007/s00203-023-03768-6
DO - 10.1007/s00203-023-03768-6
M3 - Article
C2 - 38142428
AN - SCOPUS:85180494572
SN - 0302-8933
VL - 206
JO - Archives of Microbiology
JF - Archives of Microbiology
IS - 1
M1 - 39
ER -