Pseudogenization of sopA and sopE2 is functionally linked and contributes to virulence of Salmonella enterica serovar Typhi

L. M. Valenzuela, A. A. Hidalgo, L. Rodríguez, I. M. Urrutia, A. P. Ortega, N. A. Villagra, D. Paredes-Sabja, I. L. Calderón, F. Gil, C. P. Saavedra, G. C. Mora, J. A. Fuentes

Resultado de la investigación: Article

8 Citas (Scopus)

Resumen

The difference in host range between Salmonella enterica serovar Typhimurium (S. Typhimurium) and S. enterica serovar Typhi (S. Typhi) can be partially attributed to pseudogenes. Pseudogenes are genomic segments homologous to functional genes that do not encode functional products due to the presence of genetic defects. S. Typhi lacks several protein effectors implicated in invasion or other important processes necessary for full virulence of S. Typhimurium. SopA and SopE2, effectors that have been lost by pseudogenization in S. Typhi, correspond to an ubiquitin ligase involved in cytokine production by infected cells, and to a guanine exchange factor necessary for invasion of epithelial cells, respectively. We hypothesized that sopA and/or sopE pseudogenization contributed to the virulence of S. Typhi. In this work, we found that S. Typhi expressing S. Typhimurium sopE2 exhibited a decreased invasion in different epithelial cell lines compared with S. Typhi WT. S. Typhimurium sopA completely abolished the hypo-invasive phenotype observed in S. Typhi expressing S. Typhimurium sopE2, suggesting that functional SopA and SopE2 participate concertedly in the invasion process. Finally, the expression of S. Typhimurium sopA and/or sopE2 in S. Typhi, determined changes in the secretion of IL-8 and IL-18 in infected epithelial cells.

Idioma originalEnglish
Páginas (desde-hasta)131-142
Número de páginas12
PublicaciónInfection, Genetics and Evolution
Volumen33
DOI
EstadoPublished - 1 jul 2015

Huella dactilar

Salmonella Typhi
Salmonella typhi
virulence
Virulence
serotypes
host range
epithelial cells
secretion
pseudogenes
defect
phenotype
genomics
Pseudogenes
Epithelial Cells
protein
gene
interleukin-18
guanine
interleukin-8
genetic disorders

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Microbiology (medical)
  • Infectious Diseases

Citar esto

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title = "Pseudogenization of sopA and sopE2 is functionally linked and contributes to virulence of Salmonella enterica serovar Typhi",
abstract = "The difference in host range between Salmonella enterica serovar Typhimurium (S. Typhimurium) and S. enterica serovar Typhi (S. Typhi) can be partially attributed to pseudogenes. Pseudogenes are genomic segments homologous to functional genes that do not encode functional products due to the presence of genetic defects. S. Typhi lacks several protein effectors implicated in invasion or other important processes necessary for full virulence of S. Typhimurium. SopA and SopE2, effectors that have been lost by pseudogenization in S. Typhi, correspond to an ubiquitin ligase involved in cytokine production by infected cells, and to a guanine exchange factor necessary for invasion of epithelial cells, respectively. We hypothesized that sopA and/or sopE pseudogenization contributed to the virulence of S. Typhi. In this work, we found that S. Typhi expressing S. Typhimurium sopE2 exhibited a decreased invasion in different epithelial cell lines compared with S. Typhi WT. S. Typhimurium sopA completely abolished the hypo-invasive phenotype observed in S. Typhi expressing S. Typhimurium sopE2, suggesting that functional SopA and SopE2 participate concertedly in the invasion process. Finally, the expression of S. Typhimurium sopA and/or sopE2 in S. Typhi, determined changes in the secretion of IL-8 and IL-18 in infected epithelial cells.",
keywords = "Host-specificity, Pseudogene, Reductive evolution, SopA, SopE2",
author = "Valenzuela, {L. M.} and Hidalgo, {A. A.} and L. Rodr{\'i}guez and Urrutia, {I. M.} and Ortega, {A. P.} and Villagra, {N. A.} and D. Paredes-Sabja and Calder{\'o}n, {I. L.} and F. Gil and Saavedra, {C. P.} and Mora, {G. C.} and Fuentes, {J. A.}",
year = "2015",
month = "7",
day = "1",
doi = "10.1016/j.meegid.2015.04.021",
language = "English",
volume = "33",
pages = "131--142",
journal = "Infection, Genetics and Evolution",
issn = "1567-1348",
publisher = "Elsevier",

}

TY - JOUR

T1 - Pseudogenization of sopA and sopE2 is functionally linked and contributes to virulence of Salmonella enterica serovar Typhi

AU - Valenzuela, L. M.

AU - Hidalgo, A. A.

AU - Rodríguez, L.

AU - Urrutia, I. M.

AU - Ortega, A. P.

AU - Villagra, N. A.

AU - Paredes-Sabja, D.

AU - Calderón, I. L.

AU - Gil, F.

AU - Saavedra, C. P.

AU - Mora, G. C.

AU - Fuentes, J. A.

PY - 2015/7/1

Y1 - 2015/7/1

N2 - The difference in host range between Salmonella enterica serovar Typhimurium (S. Typhimurium) and S. enterica serovar Typhi (S. Typhi) can be partially attributed to pseudogenes. Pseudogenes are genomic segments homologous to functional genes that do not encode functional products due to the presence of genetic defects. S. Typhi lacks several protein effectors implicated in invasion or other important processes necessary for full virulence of S. Typhimurium. SopA and SopE2, effectors that have been lost by pseudogenization in S. Typhi, correspond to an ubiquitin ligase involved in cytokine production by infected cells, and to a guanine exchange factor necessary for invasion of epithelial cells, respectively. We hypothesized that sopA and/or sopE pseudogenization contributed to the virulence of S. Typhi. In this work, we found that S. Typhi expressing S. Typhimurium sopE2 exhibited a decreased invasion in different epithelial cell lines compared with S. Typhi WT. S. Typhimurium sopA completely abolished the hypo-invasive phenotype observed in S. Typhi expressing S. Typhimurium sopE2, suggesting that functional SopA and SopE2 participate concertedly in the invasion process. Finally, the expression of S. Typhimurium sopA and/or sopE2 in S. Typhi, determined changes in the secretion of IL-8 and IL-18 in infected epithelial cells.

AB - The difference in host range between Salmonella enterica serovar Typhimurium (S. Typhimurium) and S. enterica serovar Typhi (S. Typhi) can be partially attributed to pseudogenes. Pseudogenes are genomic segments homologous to functional genes that do not encode functional products due to the presence of genetic defects. S. Typhi lacks several protein effectors implicated in invasion or other important processes necessary for full virulence of S. Typhimurium. SopA and SopE2, effectors that have been lost by pseudogenization in S. Typhi, correspond to an ubiquitin ligase involved in cytokine production by infected cells, and to a guanine exchange factor necessary for invasion of epithelial cells, respectively. We hypothesized that sopA and/or sopE pseudogenization contributed to the virulence of S. Typhi. In this work, we found that S. Typhi expressing S. Typhimurium sopE2 exhibited a decreased invasion in different epithelial cell lines compared with S. Typhi WT. S. Typhimurium sopA completely abolished the hypo-invasive phenotype observed in S. Typhi expressing S. Typhimurium sopE2, suggesting that functional SopA and SopE2 participate concertedly in the invasion process. Finally, the expression of S. Typhimurium sopA and/or sopE2 in S. Typhi, determined changes in the secretion of IL-8 and IL-18 in infected epithelial cells.

KW - Host-specificity

KW - Pseudogene

KW - Reductive evolution

KW - SopA

KW - SopE2

UR - http://www.scopus.com/inward/record.url?scp=84937979005&partnerID=8YFLogxK

U2 - 10.1016/j.meegid.2015.04.021

DO - 10.1016/j.meegid.2015.04.021

M3 - Article

C2 - 25913156

AN - SCOPUS:84937979005

VL - 33

SP - 131

EP - 142

JO - Infection, Genetics and Evolution

JF - Infection, Genetics and Evolution

SN - 1567-1348

ER -