Resumen
The yield of oxidative phosphorylation in isolated tomato fruit mitochondria depleted of free fatty acids remains constant when respiratory rates are decreased by a factor of 3 by the addition of n-butyl malonate. This constancy makes the determination of the contribution of the linoleic acid-induced energy-dissipating pathway by the ADP/O method possible. No decrease in membrane potential is observed in state 3 respiration with increasing concentration of n-butyl malonate, indicating that the rate of ATP synthesis is steeply dependent on membrane potential. Linoleic acid decreases the yield of oxidative phosphorylation in a concentration-dependent manner by a pure protonophoric process like that in the presence of FCCP. ADP/O measurements allow calculation of the part of respiration leading to ATP synthesis and the part of respiration sustained by the dissipative H+ re- uptake induced by linoleic acid. Respiration sustained by this energy- dissipating process remains constant at a given LA concentration until more than 50% inhibition of state 3 respiration by n-butyl malonate is achieved. The energy dissipative contribution to oxygen consumption is proposed to be equal to the protonophoric activity of plant uncoupling protein divided by the intrinsic H+/O of the cytochrome pathway. It increases with linoleic acid concentration, taking place at the expense of ADP phosphorylation without an increase in the respiration.
| Idioma original | English |
|---|---|
| Páginas (desde-hasta) | 13315-13320 |
| Número de páginas | 6 |
| Publicación | Journal of Biological Chemistry |
| Volumen | 275 |
| N.º | 18 |
| DOI | |
| Estado | Published - 5 may 2000 |
Huella dactilar
ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Molecular Biology
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Proton re-uptake partitioning between uncoupling protein and ATP synthase during benzohydroxamic acid-resistant state 3 respiration in tomato fruit mitochondria. / Jarmuszkiewicz, Wieslawa; Almeida, Andrea Miyasaka; Vercesi, Anibal E.; Sluse, Francis E.; Sluse-Goffart, Claudine M.
En: Journal of Biological Chemistry, Vol. 275, N.º 18, 05.05.2000, p. 13315-13320.Resultado de la investigación: Article
TY - JOUR
T1 - Proton re-uptake partitioning between uncoupling protein and ATP synthase during benzohydroxamic acid-resistant state 3 respiration in tomato fruit mitochondria
AU - Jarmuszkiewicz, Wieslawa
AU - Almeida, Andrea Miyasaka
AU - Vercesi, Anibal E.
AU - Sluse, Francis E.
AU - Sluse-Goffart, Claudine M.
PY - 2000/5/5
Y1 - 2000/5/5
N2 - The yield of oxidative phosphorylation in isolated tomato fruit mitochondria depleted of free fatty acids remains constant when respiratory rates are decreased by a factor of 3 by the addition of n-butyl malonate. This constancy makes the determination of the contribution of the linoleic acid-induced energy-dissipating pathway by the ADP/O method possible. No decrease in membrane potential is observed in state 3 respiration with increasing concentration of n-butyl malonate, indicating that the rate of ATP synthesis is steeply dependent on membrane potential. Linoleic acid decreases the yield of oxidative phosphorylation in a concentration-dependent manner by a pure protonophoric process like that in the presence of FCCP. ADP/O measurements allow calculation of the part of respiration leading to ATP synthesis and the part of respiration sustained by the dissipative H+ re- uptake induced by linoleic acid. Respiration sustained by this energy- dissipating process remains constant at a given LA concentration until more than 50% inhibition of state 3 respiration by n-butyl malonate is achieved. The energy dissipative contribution to oxygen consumption is proposed to be equal to the protonophoric activity of plant uncoupling protein divided by the intrinsic H+/O of the cytochrome pathway. It increases with linoleic acid concentration, taking place at the expense of ADP phosphorylation without an increase in the respiration.
AB - The yield of oxidative phosphorylation in isolated tomato fruit mitochondria depleted of free fatty acids remains constant when respiratory rates are decreased by a factor of 3 by the addition of n-butyl malonate. This constancy makes the determination of the contribution of the linoleic acid-induced energy-dissipating pathway by the ADP/O method possible. No decrease in membrane potential is observed in state 3 respiration with increasing concentration of n-butyl malonate, indicating that the rate of ATP synthesis is steeply dependent on membrane potential. Linoleic acid decreases the yield of oxidative phosphorylation in a concentration-dependent manner by a pure protonophoric process like that in the presence of FCCP. ADP/O measurements allow calculation of the part of respiration leading to ATP synthesis and the part of respiration sustained by the dissipative H+ re- uptake induced by linoleic acid. Respiration sustained by this energy- dissipating process remains constant at a given LA concentration until more than 50% inhibition of state 3 respiration by n-butyl malonate is achieved. The energy dissipative contribution to oxygen consumption is proposed to be equal to the protonophoric activity of plant uncoupling protein divided by the intrinsic H+/O of the cytochrome pathway. It increases with linoleic acid concentration, taking place at the expense of ADP phosphorylation without an increase in the respiration.
UR - http://www.scopus.com/inward/record.url?scp=0034607828&partnerID=8YFLogxK
U2 - 10.1074/jbc.275.18.13315
DO - 10.1074/jbc.275.18.13315
M3 - Article
C2 - 10788438
AN - SCOPUS:0034607828
VL - 275
SP - 13315
EP - 13320
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 18
ER -