Proregenerative microenvironment triggered by donor mesenchymal stem cells preserves renal function and structure in mice with severe diabetes mellitus

Fernando Ezquer, Maximiliano Giraud-Billoud, Daniel Carpio, Fabian Cabezas, Paulette Conget, Marcelo Ezquer

Resultado de la investigación: Contribución a una revistaArtículo

27 Citas (Scopus)

Resumen

The aim of our work was to evaluate, in an animal model of severe diabetes mellitus, the effect of mesenchymal stem cells (MSCs) administration on diabetic nephropathy (DN) progression. After diabetes induction, one group of mice received the vehicle (DM) and other group received a single dose of MSCs (DM + MSCs). DM + MSCs mice showed a significant improvement in functional parameters of the kidney compared with untreated mice. While DM mice presented marked histopathological changes characteristics of advanced stages of DN (fibrosis, glomerulosclerosis, glomerular basement membrane thickening, capillary occlusion, decreased podocyte density, and effacement of foot processes), DM + MSCs mice showed only slight tubular dilatation. The renoprotection was not associated with an improvement in diabetic condition and very low number of donor cells was found in the kidney of DM + MSCs mice, suggesting that renoprotection could be mediated by paracrine effects. Indeed, DM + MSC mice presented increased renal proliferation index, decreased renal apoptotic index and the restoration of proregenerative factors, and anti-inflammatory cytokines levels. Moreover, macrophage infiltration and oxidative stress damage were also reduced in DM + MSCs mice. Our data demonstrate that MSC administration triggers a proregenerative microenvironment in DN kidney, which allows the preservation of the renal function even if diabetes was uncorrected.

Idioma originalInglés
Número de artículo164703
PublicaciónBioMed Research International
Volumen2015
DOI
EstadoPublicada - 1 ene 2015
Publicado de forma externa

Áreas temáticas de ASJC Scopus

  • Bioquímica, genética y biología molecular (todo)
  • Inmunología y microbiología (todo)

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