Prolactin gene expression and changes of prolactin pituitary level during the seasonal acclimatization of the carp

Jaime Figueroa, Alfredo Molina, Marco Alvarez, Julieta Villanueva, Ariel Reyes, Gloria León, Manuel Krauskopf

Resultado de la investigación: Contribución a una revistaArtículo

30 Citas (Scopus)

Resumen

The effect of seasonal acclimatization on the extent of prolactin (PRL) gene expression and on the content of this was studied in summer- and winter-carp (Cyprinus carpio) hormone pituitary glands. PRL content in the rostral pars distalis (RPD) was evaluated by immunocytochemistry using antibodies against a cross-linked synthetic peptide comprising the sequence of 15 amino acids which conform to the primary structure of carp PRL. To assess the level of PRL gene transcription, a 24-mer synthetic oligonucleotide probe whose sequence included nucleotides 2041-2064 located in exon V of the carp PRL gene, was used. Employing in situ hybridization assays, a high expression of PRL mRNA was observed in the RPD of summer-acclimatized carp. A negligible level of transcription was observed in tissue sections of pituitary glands from winter-acclimatized carp. Concurrently, immunodetection of the PRL-producing cells in the RPD revealed that the pituitary hormone level was significantly higher in the warm season-adapted carp.

Idioma originalInglés
Páginas (desde-hasta)551-560
Número de páginas10
PublicaciónComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volumen108
N.º4
DOI
EstadoPublicada - 1 ene 1994

Áreas temáticas de ASJC Scopus

  • Bioquímica
  • Fisiología
  • Biología molecular

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