Prolactin gene expression and changes of prolactin pituitary level during the seasonal acclimatization of the carp

Jaime Figueroa, Alfredo Molina, Marco Alvarez, Julieta Villanueva, Ariel Reyes, Gloria León, Manuel Krauskopf

Resultado de la investigación: Article

30 Citas (Scopus)

Resumen

The effect of seasonal acclimatization on the extent of prolactin (PRL) gene expression and on the content of this was studied in summer- and winter-carp (Cyprinus carpio) hormone pituitary glands. PRL content in the rostral pars distalis (RPD) was evaluated by immunocytochemistry using antibodies against a cross-linked synthetic peptide comprising the sequence of 15 amino acids which conform to the primary structure of carp PRL. To assess the level of PRL gene transcription, a 24-mer synthetic oligonucleotide probe whose sequence included nucleotides 2041-2064 located in exon V of the carp PRL gene, was used. Employing in situ hybridization assays, a high expression of PRL mRNA was observed in the RPD of summer-acclimatized carp. A negligible level of transcription was observed in tissue sections of pituitary glands from winter-acclimatized carp. Concurrently, immunodetection of the PRL-producing cells in the RPD revealed that the pituitary hormone level was significantly higher in the warm season-adapted carp.

Idioma originalEnglish
Páginas (desde-hasta)551-560
Número de páginas10
PublicaciónComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volumen108
N.º4
DOI
EstadoPublished - 1 ene 1994

Huella dactilar

Carps
Acclimatization
Gene expression
Prolactin
Gene Expression
Pituitary Hormones
Pituitary Gland
Transcription
Genes
Anterior Pituitary Gland
Oligonucleotide Probes
In Situ Hybridization
Amino Acid Sequence
Exons
Assays
Nucleotides
Immunohistochemistry
Hormones
Tissue
Amino Acids

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

Citar esto

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title = "Prolactin gene expression and changes of prolactin pituitary level during the seasonal acclimatization of the carp",
abstract = "The effect of seasonal acclimatization on the extent of prolactin (PRL) gene expression and on the content of this was studied in summer- and winter-carp (Cyprinus carpio) hormone pituitary glands. PRL content in the rostral pars distalis (RPD) was evaluated by immunocytochemistry using antibodies against a cross-linked synthetic peptide comprising the sequence of 15 amino acids which conform to the primary structure of carp PRL. To assess the level of PRL gene transcription, a 24-mer synthetic oligonucleotide probe whose sequence included nucleotides 2041-2064 located in exon V of the carp PRL gene, was used. Employing in situ hybridization assays, a high expression of PRL mRNA was observed in the RPD of summer-acclimatized carp. A negligible level of transcription was observed in tissue sections of pituitary glands from winter-acclimatized carp. Concurrently, immunodetection of the PRL-producing cells in the RPD revealed that the pituitary hormone level was significantly higher in the warm season-adapted carp.",
keywords = "Carp, Cyprinus carpio, Gene expression, Pituitary, Prolactin, Seasonal acclimatization, Teleost fish, Temperature",
author = "Jaime Figueroa and Alfredo Molina and Marco Alvarez and Julieta Villanueva and Ariel Reyes and Gloria Le{\'o}n and Manuel Krauskopf",
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Prolactin gene expression and changes of prolactin pituitary level during the seasonal acclimatization of the carp. / Figueroa, Jaime; Molina, Alfredo; Alvarez, Marco; Villanueva, Julieta; Reyes, Ariel; León, Gloria; Krauskopf, Manuel.

En: Comparative Biochemistry and Physiology -- Part B: Biochemistry and, Vol. 108, N.º 4, 01.01.1994, p. 551-560.

Resultado de la investigación: Article

TY - JOUR

T1 - Prolactin gene expression and changes of prolactin pituitary level during the seasonal acclimatization of the carp

AU - Figueroa, Jaime

AU - Molina, Alfredo

AU - Alvarez, Marco

AU - Villanueva, Julieta

AU - Reyes, Ariel

AU - León, Gloria

AU - Krauskopf, Manuel

PY - 1994/1/1

Y1 - 1994/1/1

N2 - The effect of seasonal acclimatization on the extent of prolactin (PRL) gene expression and on the content of this was studied in summer- and winter-carp (Cyprinus carpio) hormone pituitary glands. PRL content in the rostral pars distalis (RPD) was evaluated by immunocytochemistry using antibodies against a cross-linked synthetic peptide comprising the sequence of 15 amino acids which conform to the primary structure of carp PRL. To assess the level of PRL gene transcription, a 24-mer synthetic oligonucleotide probe whose sequence included nucleotides 2041-2064 located in exon V of the carp PRL gene, was used. Employing in situ hybridization assays, a high expression of PRL mRNA was observed in the RPD of summer-acclimatized carp. A negligible level of transcription was observed in tissue sections of pituitary glands from winter-acclimatized carp. Concurrently, immunodetection of the PRL-producing cells in the RPD revealed that the pituitary hormone level was significantly higher in the warm season-adapted carp.

AB - The effect of seasonal acclimatization on the extent of prolactin (PRL) gene expression and on the content of this was studied in summer- and winter-carp (Cyprinus carpio) hormone pituitary glands. PRL content in the rostral pars distalis (RPD) was evaluated by immunocytochemistry using antibodies against a cross-linked synthetic peptide comprising the sequence of 15 amino acids which conform to the primary structure of carp PRL. To assess the level of PRL gene transcription, a 24-mer synthetic oligonucleotide probe whose sequence included nucleotides 2041-2064 located in exon V of the carp PRL gene, was used. Employing in situ hybridization assays, a high expression of PRL mRNA was observed in the RPD of summer-acclimatized carp. A negligible level of transcription was observed in tissue sections of pituitary glands from winter-acclimatized carp. Concurrently, immunodetection of the PRL-producing cells in the RPD revealed that the pituitary hormone level was significantly higher in the warm season-adapted carp.

KW - Carp

KW - Cyprinus carpio

KW - Gene expression

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KW - Prolactin

KW - Seasonal acclimatization

KW - Teleost fish

KW - Temperature

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