Proenkephalin A and the γ-aminobutyric acid A receptor π subunit: expression, localization, and dynamic changes in human secretory endometrium

Marisol Quezada, Soledad Henríquez, Macarena Vargas, Hugo Cardenas, Alejandro Tapia, Miguel Rios, Ana M. Salvatierra, Pedro A. Orihuela, Horacio B. Croxatto, Luis Velasquez

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

10 Citas (Scopus)

Resumen

Objective: To compare mRNA and protein levels of proenkephalin A (PEA) and γ-aminobutyric acid A receptor π subunit (πGABA-R) in human secretory endometrium before and during receptivity and to determine the cell phenotypes where they are expressed. Design: Prospective and observational, comparing prereceptive vs. receptive stages of secretory endometrium within the same nonconceptional menstrual cycle. Setting: University and non-governmental organization (NGO)-based academic and clinical-research facilities. Patient(s): Seven healthy, multiparous, surgically sterilized women with spontaneous regular menstrual cycles. Intervention(s): Endometrial biopsies were obtained on LH+3 and LH+7 within the same cycle. Main Outcome Measure(s): Levels of PEA and πGABA-R mRNA were determined by real-time PCR, and protein presence, by immunofluorescence. Result(s): The mRNA level of PEA fell, whereas that of πGABA-R increased, during endometrial receptivity. Positive immunostaining of PEA was found in the luminal and glandular epithelium, whereas that of πGABA-R was in luminal epithelium and stromal cells. Conclusion(s): The discrete cell-phenotype localization and timing of the changes in the level of PEA and of πGABA-R mRNA and protein suggest an important role for these molecules in switching the human endometrium from a refractory to a receptive state.

Idioma originalInglés
Páginas (desde-hasta)1750-1757
Número de páginas8
PublicaciónFertility and Sterility
Volumen86
N.º6
DOI
EstadoPublicada - dic 2006

Áreas temáticas de ASJC Scopus

  • Medicina reproductiva
  • Ginecología y obstetricia

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